Chlorine in PDB 3hwl: Crystal Structure of T4 Lysozyme with the Unnatural Amino Acid P- Acetyl-L-Phenylalanine Incorporated at Position 131

Enzymatic activity of Crystal Structure of T4 Lysozyme with the Unnatural Amino Acid P- Acetyl-L-Phenylalanine Incorporated at Position 131

All present enzymatic activity of Crystal Structure of T4 Lysozyme with the Unnatural Amino Acid P- Acetyl-L-Phenylalanine Incorporated at Position 131:
3.2.1.17;

Protein crystallography data

The structure of Crystal Structure of T4 Lysozyme with the Unnatural Amino Acid P- Acetyl-L-Phenylalanine Incorporated at Position 131, PDB code: 3hwl was solved by M.R.Fleissner, D.Cascio, P.G.Schultz, W.L.Hubbell, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 35.31 / 1.80
Space group P 32 2 1
Cell size a, b, c (Å), α, β, γ (°) 59.972, 59.972, 96.282, 90.00, 90.00, 120.00
R / Rfree (%) 18.7 / 22.7

Chlorine Binding Sites:

The binding sites of Chlorine atom in the Crystal Structure of T4 Lysozyme with the Unnatural Amino Acid P- Acetyl-L-Phenylalanine Incorporated at Position 131 (pdb code 3hwl). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 3 binding sites of Chlorine where determined in the Crystal Structure of T4 Lysozyme with the Unnatural Amino Acid P- Acetyl-L-Phenylalanine Incorporated at Position 131, PDB code: 3hwl:
Jump to Chlorine binding site number: 1; 2; 3;

Chlorine binding site 1 out of 3 in 3hwl

Go back to Chlorine Binding Sites List in 3hwl
Chlorine binding site 1 out of 3 in the Crystal Structure of T4 Lysozyme with the Unnatural Amino Acid P- Acetyl-L-Phenylalanine Incorporated at Position 131


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Crystal Structure of T4 Lysozyme with the Unnatural Amino Acid P- Acetyl-L-Phenylalanine Incorporated at Position 131 within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Cl167

b:35.8
occ:1.00
 CE A:LYS48 3.2 32.7 1.0
OE2 A:GLU45 3.4 40.0 1.0
OG A:SER44 3.4 32.4 1.0
CD A:LYS48 3.8 24.3 1.0
O A:SER44 4.1 19.2 1.0
CD A:GLU45 4.1 30.4 1.0
C A:SER44 4.3 22.0 1.0
NZ A:LYS48 4.3 41.9 1.0
CB A:SER44 4.4 29.4 1.0
O A:HOH217 4.4 34.6 1.0
CB A:LYS48 4.4 22.5 1.0
N A:GLU45 4.5 18.9 1.0
CA A:GLU45 4.6 20.2 1.0
OE1 A:GLU45 4.6 29.4 1.0
CG A:LYS48 4.8 23.7 1.0
CG A:GLU45 4.9 24.7 1.0
CA A:SER44 5.0 22.3 1.0

Chlorine binding site 2 out of 3 in 3hwl

Go back to Chlorine Binding Sites List in 3hwl
Chlorine binding site 2 out of 3 in the Crystal Structure of T4 Lysozyme with the Unnatural Amino Acid P- Acetyl-L-Phenylalanine Incorporated at Position 131


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Crystal Structure of T4 Lysozyme with the Unnatural Amino Acid P- Acetyl-L-Phenylalanine Incorporated at Position 131 within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Cl168

b:29.6
occ:1.00
 O A:HOH240 2.6 32.3 1.0
O A:HOH236 3.0 23.1 1.0
N A:ARG145 3.2 14.9 1.0
C A:THR142 3.4 18.3 1.0
N A:ASN144 3.4 17.8 1.0
CA A:THR142 3.5 17.6 1.0
CB A:THR142 3.6 21.3 1.0
O A:THR142 3.6 16.9 1.0
N A:PRO143 3.8 18.0 1.0
CB A:ASN144 3.8 19.3 1.0
CB A:ARG145 3.8 18.0 1.0
O A:HOH235 3.8 35.2 1.0
CA A:ASN144 3.9 17.4 1.0
C A:ASN144 4.0 18.0 1.0
CA A:ARG145 4.1 15.8 1.0
CD A:PRO143 4.1 21.0 1.0
CG2 A:THR142 4.2 23.1 1.0
C A:PRO143 4.3 19.5 1.0
CA A:PRO143 4.6 17.9 1.0
O A:HOH201 4.7 26.6 1.0
CG A:ASN144 4.7 25.1 1.0
OG1 A:THR142 4.8 21.7 1.0
N A:THR142 4.9 19.1 1.0

Chlorine binding site 3 out of 3 in 3hwl

Go back to Chlorine Binding Sites List in 3hwl
Chlorine binding site 3 out of 3 in the Crystal Structure of T4 Lysozyme with the Unnatural Amino Acid P- Acetyl-L-Phenylalanine Incorporated at Position 131


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 3 of Crystal Structure of T4 Lysozyme with the Unnatural Amino Acid P- Acetyl-L-Phenylalanine Incorporated at Position 131 within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Cl169

b:64.8
occ:1.00
 NH1 A:ARG148 3.3 19.3 1.0
ND2 A:ASN144 3.8 29.3 1.0
CB A:ASN144 3.8 19.3 1.0
O A:HOH235 4.0 35.2 1.0
NH2 A:ARG148 4.2 20.5 1.0
CZ A:ARG148 4.2 23.1 1.0
CG A:ASN144 4.3 25.1 1.0
O A:HOH183 4.8 24.1 1.0

Reference:

M.R.Fleissner, E.M.Brustad, T.Kalai, C.Altenbach, D.Cascio, F.B.Peters, K.Hideg, S.Peuker, P.G.Schultz, W.L.Hubbell. Site-Directed Spin Labeling of A Genetically Encoded Unnatural Amino Acid. Proc.Natl.Acad.Sci.Usa V. 106 21637 2009.
ISSN: ISSN 0027-8424
PubMed: 19995976
DOI: 10.1073/PNAS.0912009106
Page generated: Sat Jul 20 21:07:08 2024

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