Chlorine in PDB 3l29: Crystal Structure of Zaire Ebola VP35 Interferon Inhibitory Domain K319A/R322A Mutant

The structure of Crystal Structure of Zaire Ebola VP35 Interferon Inhibitory Domain K319A/R322A Mutant, PDB code: 3l29 was solved by D.W.Leung, P.Ramanan, D.M.Borek, G.K.Amarasinghe, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	36.32 / 1.70
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	51.430, 66.070, 72.640, 90.00, 90.00, 90.00
R / Rfree (%)	19.1 / 23.2

The binding sites of Chlorine atom in the Crystal Structure of Zaire Ebola VP35 Interferon Inhibitory Domain K319A/R322A Mutant (pdb code 3l29). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 2 binding sites of Chlorine where determined in the Crystal Structure of Zaire Ebola VP35 Interferon Inhibitory Domain K319A/R322A Mutant, PDB code: 3l29:
Jump to Chlorine binding site number: 1; 2;

Chlorine binding site 1 out of 2 in the Crystal Structure of Zaire Ebola VP35 Interferon Inhibitory Domain K319A/R322A Mutant


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Crystal Structure of Zaire Ebola VP35 Interferon Inhibitory Domain K319A/R322A Mutant within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl2 b:30.0 occ:1.00 NE A:ARG225 2.9 22.6 1.0 OD1 A:ASN226 3.3 26.0 1.0 CB A:ARG225 3.5 18.7 1.0 CE2 A:TYR229 3.7 24.6 1.0 NH2 A:ARG225 3.7 19.3 1.0 CZ A:ARG225 3.7 21.7 1.0 O A:HOH86 3.8 29.4 1.0 CD A:ARG225 3.8 19.5 1.0 CG A:ASN226 3.8 23.5 1.0 ND2 A:ASN226 4.0 24.3 1.0 O A:LYS222 4.2 18.3 1.0 CG A:ARG225 4.3 19.0 1.0 CD2 A:TYR229 4.3 24.6 1.0 CG A:LYS222 4.4 20.9 1.0 N A:ASN226 4.4 19.6 1.0 OH A:TYR229 4.6 22.8 1.0 CZ A:TYR229 4.6 24.1 1.0 CA A:LYS222 4.7 19.6 1.0 C A:ARG225 4.7 19.4 1.0 CA A:ARG225 4.7 19.5 1.0 C A:LYS222 4.9 18.9 1.0 NH1 A:ARG225 5.0 22.1 1.0 CB A:ASN226 5.0 19.4 1.0

Chlorine binding site 2 out of 2 in the Crystal Structure of Zaire Ebola VP35 Interferon Inhibitory Domain K319A/R322A Mutant


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Crystal Structure of Zaire Ebola VP35 Interferon Inhibitory Domain K319A/R322A Mutant within 5.0Å range: probe atom residue distance (Å) B Occ B:Cl1 b:61.9 occ:1.00 NE B:ARG225 3.7 26.1 1.0 NH2 B:ARG225 3.8 28.9 1.0 OD1 B:ASN226 3.9 23.9 1.0 CZ B:ARG225 4.2 27.7 1.0 CG B:LYS222 4.3 22.4 1.0 NZ B:LYS222 4.3 31.0 1.0 ND2 B:ASN226 4.5 25.5 1.0 CG B:ASN226 4.5 20.1 1.0 CD B:ARG225 4.7 22.9 1.0 CE2 B:TYR229 4.7 24.1 1.0 CB B:ARG225 4.8 21.0 1.0 CD B:LYS222 4.8 26.2 1.0 O B:LYS222 5.0 19.8 1.0

K.C.Prins, S.Delpeut, D.W.Leung, O.Reynard, V.A.Volchkova, S.P.Reid, P.Ramanan, W.B.Cardenas, G.K.Amarasinghe, V.E.Volchkov, C.F.Basler. Mutations Abrogating VP35 Interaction with Double-Stranded Rna Render Ebola Virus Avirulent in Guinea Pigs. J.Virol. V. 84 3004 2010.
ISSN: ISSN 0022-538X
PubMed: 20071589
DOI: 10.1128/JVI.02459-09