Chlorine in PDB 6gos: E. Coli Microcin Synthetase Mcbbcd Complex with Pro-MCCB17 Bound

The structure of E. Coli Microcin Synthetase Mcbbcd Complex with Pro-MCCB17 Bound, PDB code: 6gos was solved by D.Ghilarov, C.E.M.Stevenson, D.Y.Travin, J.Piskunova, M.Serebryakova, A.Maxwell, D.M.Lawson, K.Severinov, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	57.39 / 2.10
Space group	C 1 2 1
Cell size a, b, c (Å), α, β, γ (°)	180.960, 83.400, 86.900, 90.00, 91.45, 90.00
R / Rfree (%)	17.2 / 21.2

The structure of E. Coli Microcin Synthetase Mcbbcd Complex with Pro-MCCB17 Bound also contains other interesting chemical elements:

Zinc

(Zn)

2 atoms

The binding sites of Chlorine atom in the E. Coli Microcin Synthetase Mcbbcd Complex with Pro-MCCB17 Bound (pdb code 6gos). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 2 binding sites of Chlorine where determined in the E. Coli Microcin Synthetase Mcbbcd Complex with Pro-MCCB17 Bound, PDB code: 6gos:
Jump to Chlorine binding site number: 1; 2;

Chlorine binding site 1 out of 2 in the E. Coli Microcin Synthetase Mcbbcd Complex with Pro-MCCB17 Bound


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of E. Coli Microcin Synthetase Mcbbcd Complex with Pro-MCCB17 Bound within 5.0Å range: probe atom residue distance (Å) B Occ 1:Cl303 b:58.1 occ:1.00 NZ 1:LYS159 2.8 72.1 1.0 NH2 1:ARG155 3.5 64.4 1.0 CE 1:LYS159 3.7 62.4 1.0 N 1:ASN132 3.8 74.5 1.0 NE 1:ARG155 4.0 64.3 1.0 CD 1:LYS159 4.1 57.6 1.0 CZ 1:ARG155 4.2 64.2 1.0 CA 1:ASN131 4.3 66.9 1.0 C 1:ASN131 4.5 71.0 1.0 CA 1:ASN132 4.7 77.1 1.0 O 1:ASP130 4.8 61.0 1.0 N 1:ILE133 4.9 72.7 1.0 CB 1:ASN131 4.9 68.1 1.0 CG 1:LYS159 4.9 52.9 1.0

Chlorine binding site 2 out of 2 in the E. Coli Microcin Synthetase Mcbbcd Complex with Pro-MCCB17 Bound


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of E. Coli Microcin Synthetase Mcbbcd Complex with Pro-MCCB17 Bound within 5.0Å range: probe atom residue distance (Å) B Occ D:Cl402 b:68.4 occ:1.00 CD D:LYS253 3.5 55.2 1.0 N D:ASN249 3.6 47.2 1.0 N D:SER250 3.6 48.7 1.0 CB D:ASN249 3.8 56.7 1.0 CB D:ALA248 4.0 42.1 1.0 CB D:SER250 4.1 47.0 1.0 CA D:ASN249 4.1 50.8 1.0 NZ D:LYS253 4.2 63.1 1.0 CE D:LYS253 4.3 59.1 1.0 C D:ASN249 4.4 49.5 1.0 C D:ALA248 4.5 47.0 1.0 CA D:SER250 4.5 47.0 1.0 ND2 D:ASN249 4.5 68.3 1.0 CG D:LYS253 4.6 49.8 1.0 CA D:ALA248 4.6 43.7 1.0 CG D:ASN249 4.6 64.7 1.0 CB D:LYS253 4.7 44.8 1.0

D.Ghilarov, C.E.M.Stevenson, D.Y.Travin, J.Piskunova, M.Serebryakova, A.Maxwell, D.M.Lawson, K.Severinov. Architecture of Microcin B17 Synthetase: An Octameric Protein Complex Converting A Ribosomally Synthesized Peptide Into A Dna Gyrase Poison. Mol. Cell V. 73 749 2019.
ISSN: ISSN 1097-4164
PubMed: 30661981
DOI: 10.1016/J.MOLCEL.2018.11.032