Chlorine in PDB 7kt4: Dna Polymerase Mu, 8-Oxodgtp:at Reaction State Ternary Complex, 10 Mm MN2+ (30MIN)

All present enzymatic activity of Dna Polymerase Mu, 8-Oxodgtp:at Reaction State Ternary Complex, 10 Mm MN2+ (30MIN):
2.7.7.7;

The structure of Dna Polymerase Mu, 8-Oxodgtp:at Reaction State Ternary Complex, 10 Mm MN2+ (30MIN), PDB code: 7kt4 was solved by J.A.Jamsen, S.H.Wilson, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	21.43 / 1.92
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	60.119, 68.819, 110.296, 90, 90, 90
R / Rfree (%)	16.6 / 20.4

The structure of Dna Polymerase Mu, 8-Oxodgtp:at Reaction State Ternary Complex, 10 Mm MN2+ (30MIN) also contains other interesting chemical elements:

Sodium	(Na)	1 atom
Manganese	(Mn)	4 atoms

The binding sites of Chlorine atom in the Dna Polymerase Mu, 8-Oxodgtp:at Reaction State Ternary Complex, 10 Mm MN2+ (30MIN) (pdb code 7kt4). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 2 binding sites of Chlorine where determined in the Dna Polymerase Mu, 8-Oxodgtp:at Reaction State Ternary Complex, 10 Mm MN2+ (30MIN), PDB code: 7kt4:
Jump to Chlorine binding site number: 1; 2;

Chlorine binding site 1 out of 2 in the Dna Polymerase Mu, 8-Oxodgtp:at Reaction State Ternary Complex, 10 Mm MN2+ (30MIN)


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Dna Polymerase Mu, 8-Oxodgtp:at Reaction State Ternary Complex, 10 Mm MN2+ (30MIN) within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl507 b:26.5 occ:1.00 OG A:SER458 2.8 25.2 1.0 ND1 A:HIS459 3.1 46.2 1.0 N A:SER458 3.3 14.5 1.0 O P:HOH207 3.4 20.2 1.0 CE1 A:HIS459 3.4 47.9 1.0 C1' T:DA7 3.5 23.4 1.0 O4' T:DA7 3.5 25.0 1.0 NH2 A:ARG387 3.6 22.4 1.0 C2' T:DA7 3.6 23.6 1.0 C4' T:DA7 3.6 22.1 1.0 CB A:SER458 3.6 21.5 1.0 CB A:ASN457 3.7 17.8 1.0 CA A:SER458 3.8 14.1 1.0 CG A:ASN457 3.9 21.8 1.0 C3' T:DA7 4.0 23.2 1.0 O3' T:DA7 4.2 25.7 1.0 N A:HIS459 4.2 15.2 1.0 C A:SER458 4.2 14.3 1.0 ND2 A:ASN457 4.3 31.2 1.0 CG A:HIS459 4.3 29.9 1.0 C A:ASN457 4.3 17.3 1.0 OD1 A:ASN457 4.4 18.1 1.0 CA A:ASN457 4.5 16.7 1.0 NE2 A:HIS459 4.6 37.4 1.0 CZ A:ARG387 4.8 23.3 1.0 O2 P:EDO101 4.9 25.1 1.0 N9 T:DA7 4.9 27.0 1.0 NH2 A:ARG445 4.9 13.7 1.0 C5' T:DA7 4.9 29.0 1.0 O A:HOH673 4.9 17.1 1.0 CB A:HIS459 5.0 14.4 1.0

Chlorine binding site 2 out of 2 in the Dna Polymerase Mu, 8-Oxodgtp:at Reaction State Ternary Complex, 10 Mm MN2+ (30MIN)


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Dna Polymerase Mu, 8-Oxodgtp:at Reaction State Ternary Complex, 10 Mm MN2+ (30MIN) within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl508 b:52.2 occ:0.79 OG A:SER172 2.3 34.6 0.2 O A:HOH745 2.5 42.0 1.0 N A:GLY174 3.3 21.9 1.0 CB A:SER172 3.4 36.2 0.2 N A:ARG175 3.4 18.2 1.0 CB A:SER172 3.4 39.0 0.8 CA A:SER172 3.5 29.7 0.2 CA A:SER172 3.5 29.2 0.8 CA A:GLY174 3.6 19.9 1.0 C A:SER172 3.6 23.7 0.8 C A:SER172 3.6 25.2 0.2 CG A:ARG175 3.9 25.7 1.0 N A:GLU173 4.0 25.4 1.0 O6 D:DG1 4.0 21.8 1.0 C A:GLY174 4.0 20.2 1.0 O A:SER172 4.1 23.2 0.8 O A:SER172 4.1 22.8 0.2 O D:HOH110 4.2 23.6 0.5 C6 D:DG1 4.3 21.1 1.0 OG A:SER172 4.4 54.5 0.8 C A:GLU173 4.4 17.6 1.0 CB A:ARG175 4.4 18.7 1.0 O A:HOH781 4.4 41.9 1.0 CA A:ARG175 4.5 18.0 1.0 C5 D:DG1 4.7 22.0 1.0 N7 D:DG1 4.7 24.8 1.0 CD A:ARG175 4.8 42.0 1.0 CA A:GLU173 4.8 25.3 1.0 N A:SER172 4.9 29.2 0.2 N A:SER172 4.9 27.4 0.8 N4 T:DC4 5.0 22.9 1.0

J.A.Jamsen, A.Sassa, D.D.Shock, W.A.Beard, S.H.Wilson. Dna Polymerase Mu, 8-Oxodgtp:at Reaction State Ternary Complex, 10 Mm MN2+ (30MIN) To Be Published.