Chlorine in PDB 7kt4: Dna Polymerase Mu, 8-Oxodgtp:at Reaction State Ternary Complex, 10 Mm MN2+ (30MIN)

Enzymatic activity of Dna Polymerase Mu, 8-Oxodgtp:at Reaction State Ternary Complex, 10 Mm MN2+ (30MIN)

All present enzymatic activity of Dna Polymerase Mu, 8-Oxodgtp:at Reaction State Ternary Complex, 10 Mm MN2+ (30MIN):
2.7.7.7;

Protein crystallography data

The structure of Dna Polymerase Mu, 8-Oxodgtp:at Reaction State Ternary Complex, 10 Mm MN2+ (30MIN), PDB code: 7kt4 was solved by J.A.Jamsen, S.H.Wilson, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 21.43 / 1.92
Space group P 21 21 21
Cell size a, b, c (Å), α, β, γ (°) 60.119, 68.819, 110.296, 90, 90, 90
R / Rfree (%) 16.6 / 20.4

Other elements in 7kt4:

The structure of Dna Polymerase Mu, 8-Oxodgtp:at Reaction State Ternary Complex, 10 Mm MN2+ (30MIN) also contains other interesting chemical elements:

Sodium (Na) 1 atom
Manganese (Mn) 4 atoms

Chlorine Binding Sites:

The binding sites of Chlorine atom in the Dna Polymerase Mu, 8-Oxodgtp:at Reaction State Ternary Complex, 10 Mm MN2+ (30MIN) (pdb code 7kt4). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 2 binding sites of Chlorine where determined in the Dna Polymerase Mu, 8-Oxodgtp:at Reaction State Ternary Complex, 10 Mm MN2+ (30MIN), PDB code: 7kt4:
Jump to Chlorine binding site number: 1; 2;

Chlorine binding site 1 out of 2 in 7kt4

Go back to Chlorine Binding Sites List in 7kt4
Chlorine binding site 1 out of 2 in the Dna Polymerase Mu, 8-Oxodgtp:at Reaction State Ternary Complex, 10 Mm MN2+ (30MIN)


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Dna Polymerase Mu, 8-Oxodgtp:at Reaction State Ternary Complex, 10 Mm MN2+ (30MIN) within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Cl507

b:26.5
occ:1.00
OG A:SER458 2.8 25.2 1.0
ND1 A:HIS459 3.1 46.2 1.0
N A:SER458 3.3 14.5 1.0
O P:HOH207 3.4 20.2 1.0
CE1 A:HIS459 3.4 47.9 1.0
C1' T:DA7 3.5 23.4 1.0
O4' T:DA7 3.5 25.0 1.0
NH2 A:ARG387 3.6 22.4 1.0
C2' T:DA7 3.6 23.6 1.0
C4' T:DA7 3.6 22.1 1.0
CB A:SER458 3.6 21.5 1.0
CB A:ASN457 3.7 17.8 1.0
CA A:SER458 3.8 14.1 1.0
CG A:ASN457 3.9 21.8 1.0
C3' T:DA7 4.0 23.2 1.0
O3' T:DA7 4.2 25.7 1.0
N A:HIS459 4.2 15.2 1.0
C A:SER458 4.2 14.3 1.0
ND2 A:ASN457 4.3 31.2 1.0
CG A:HIS459 4.3 29.9 1.0
C A:ASN457 4.3 17.3 1.0
OD1 A:ASN457 4.4 18.1 1.0
CA A:ASN457 4.5 16.7 1.0
NE2 A:HIS459 4.6 37.4 1.0
CZ A:ARG387 4.8 23.3 1.0
O2 P:EDO101 4.9 25.1 1.0
N9 T:DA7 4.9 27.0 1.0
NH2 A:ARG445 4.9 13.7 1.0
C5' T:DA7 4.9 29.0 1.0
O A:HOH673 4.9 17.1 1.0
CB A:HIS459 5.0 14.4 1.0

Chlorine binding site 2 out of 2 in 7kt4

Go back to Chlorine Binding Sites List in 7kt4
Chlorine binding site 2 out of 2 in the Dna Polymerase Mu, 8-Oxodgtp:at Reaction State Ternary Complex, 10 Mm MN2+ (30MIN)


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Dna Polymerase Mu, 8-Oxodgtp:at Reaction State Ternary Complex, 10 Mm MN2+ (30MIN) within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Cl508

b:52.2
occ:0.79
OG A:SER172 2.3 34.6 0.2
O A:HOH745 2.5 42.0 1.0
N A:GLY174 3.3 21.9 1.0
CB A:SER172 3.4 36.2 0.2
N A:ARG175 3.4 18.2 1.0
CB A:SER172 3.4 39.0 0.8
CA A:SER172 3.5 29.7 0.2
CA A:SER172 3.5 29.2 0.8
CA A:GLY174 3.6 19.9 1.0
C A:SER172 3.6 23.7 0.8
C A:SER172 3.6 25.2 0.2
CG A:ARG175 3.9 25.7 1.0
N A:GLU173 4.0 25.4 1.0
O6 D:DG1 4.0 21.8 1.0
C A:GLY174 4.0 20.2 1.0
O A:SER172 4.1 23.2 0.8
O A:SER172 4.1 22.8 0.2
O D:HOH110 4.2 23.6 0.5
C6 D:DG1 4.3 21.1 1.0
OG A:SER172 4.4 54.5 0.8
C A:GLU173 4.4 17.6 1.0
CB A:ARG175 4.4 18.7 1.0
O A:HOH781 4.4 41.9 1.0
CA A:ARG175 4.5 18.0 1.0
C5 D:DG1 4.7 22.0 1.0
N7 D:DG1 4.7 24.8 1.0
CD A:ARG175 4.8 42.0 1.0
CA A:GLU173 4.8 25.3 1.0
N A:SER172 4.9 29.2 0.2
N A:SER172 4.9 27.4 0.8
N4 T:DC4 5.0 22.9 1.0

Reference:

J.A.Jamsen, A.Sassa, D.D.Shock, W.A.Beard, S.H.Wilson. Dna Polymerase Mu, 8-Oxodgtp:at Reaction State Ternary Complex, 10 Mm MN2+ (30MIN) To Be Published.
Page generated: Fri Dec 17 08:51:21 2021

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