Chlorine in PDB 7lca: Zoogloea Ramigera Biosynthetic Thiolase Y218E/DELH221 Mutant

All present enzymatic activity of Zoogloea Ramigera Biosynthetic Thiolase Y218E/DELH221 Mutant:
2.3.1.9;

The structure of Zoogloea Ramigera Biosynthetic Thiolase Y218E/DELH221 Mutant, PDB code: 7lca was solved by A.C.Marshall, J.B.Bruning, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	46.43 / 2.00
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	84.45, 79.34, 149.14, 90, 92.86, 90
R / Rfree (%)	26.1 / 28.3

The binding sites of Chlorine atom in the Zoogloea Ramigera Biosynthetic Thiolase Y218E/DELH221 Mutant (pdb code 7lca). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 2 binding sites of Chlorine where determined in the Zoogloea Ramigera Biosynthetic Thiolase Y218E/DELH221 Mutant, PDB code: 7lca:
Jump to Chlorine binding site number: 1; 2;

Chlorine binding site 1 out of 2 in the Zoogloea Ramigera Biosynthetic Thiolase Y218E/DELH221 Mutant


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Zoogloea Ramigera Biosynthetic Thiolase Y218E/DELH221 Mutant within 5.0Å range: probe atom residue distance (Å) B Occ B:Cl405 b:28.8 occ:1.00 N B:GLY47 3.0 13.7 1.0 CA B:GLY47 3.5 14.5 1.0 CB B:ALA45 3.5 13.2 1.0 N B:ALA46 3.8 13.1 1.0 C B:ALA46 4.1 13.1 1.0 C B:ALA45 4.3 17.0 1.0 O B:HOH578 4.3 17.3 1.0 CA B:ALA46 4.4 15.7 1.0 CA B:ALA45 4.4 13.1 1.0 O B:HOH534 4.5 28.7 1.0 C B:GLY47 4.6 17.2 1.0 CB B:ALA46 4.7 17.6 1.0

Chlorine binding site 2 out of 2 in the Zoogloea Ramigera Biosynthetic Thiolase Y218E/DELH221 Mutant


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Zoogloea Ramigera Biosynthetic Thiolase Y218E/DELH221 Mutant within 5.0Å range: probe atom residue distance (Å) B Occ B:Cl406 b:26.8 occ:1.00 O B:HOH553 2.6 20.4 1.0 N B:SER259 3.0 13.6 1.0 O B:SER109 3.1 13.2 1.0 OG B:SER259 3.2 17.2 1.0 CB B:SER259 3.4 13.6 1.0 CB B:GLU262 3.5 15.9 1.0 CG2 B:ILE103 3.7 13.2 1.0 CA B:SER259 3.8 13.6 1.0 CG B:GLU262 3.8 20.8 1.0 CA B:SER109 3.8 15.8 1.0 C B:SER109 3.8 15.8 1.0 C B:MET258 3.9 13.6 1.0 CA B:MET258 4.0 13.6 1.0 CB B:MET258 4.0 13.6 1.0 O3 B:SO4402 4.1 40.1 1.0 CB B:SER109 4.1 16.1 1.0 OE1 B:GLU262 4.4 24.1 1.0 CD B:GLU262 4.4 25.6 1.0 OG B:SER109 4.5 23.3 1.0 O B:HOH550 4.7 26.1 1.0 O4 B:SO4402 4.7 39.9 1.0 C B:SER259 4.8 16.3 1.0 O B:SER259 4.8 15.7 1.0 CA B:GLU262 4.9 17.8 1.0 CD1 B:ILE103 4.9 13.9 1.0 CD1 B:ILE110 5.0 22.9 1.0

A.C.Marshall, J.B.Bruning. Engineering Potassium Activation Into Biosynthetic Thiolase. Biochem.J. V. 478 3047 2021.
ISSN: ESSN 1470-8728
PubMed: 34338286
DOI: 10.1042/BCJ20210455