Chlorine in PDB 7ldv: Zoogloea Ramigera Biosynthetic Thiolase Q183Y/Y218E/DELH221/S227K Mutant

Enzymatic activity of Zoogloea Ramigera Biosynthetic Thiolase Q183Y/Y218E/DELH221/S227K Mutant

All present enzymatic activity of Zoogloea Ramigera Biosynthetic Thiolase Q183Y/Y218E/DELH221/S227K Mutant:
2.3.1.9;

Protein crystallography data

The structure of Zoogloea Ramigera Biosynthetic Thiolase Q183Y/Y218E/DELH221/S227K Mutant, PDB code: 7ldv was solved by A.C.Marshall, J.B.Bruning, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 47.23 / 2.90
Space group P 1 21 1
Cell size a, b, c (Å), α, β, γ (°) 84.764, 78.965, 152.882, 90, 94.49, 90
R / Rfree (%) 19.6 / 23.4

Chlorine Binding Sites:

The binding sites of Chlorine atom in the Zoogloea Ramigera Biosynthetic Thiolase Q183Y/Y218E/DELH221/S227K Mutant (pdb code 7ldv). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 6 binding sites of Chlorine where determined in the Zoogloea Ramigera Biosynthetic Thiolase Q183Y/Y218E/DELH221/S227K Mutant, PDB code: 7ldv:
Jump to Chlorine binding site number: 1; 2; 3; 4; 5; 6;

Chlorine binding site 1 out of 6 in 7ldv

Go back to Chlorine Binding Sites List in 7ldv
Chlorine binding site 1 out of 6 in the Zoogloea Ramigera Biosynthetic Thiolase Q183Y/Y218E/DELH221/S227K Mutant


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Zoogloea Ramigera Biosynthetic Thiolase Q183Y/Y218E/DELH221/S227K Mutant within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Cl406

b:58.1
occ:1.00
 O A:HOH544 3.4 23.2 1.0
NH2 A:ARG220 3.5 81.7 1.0
NZ A:LYS226 3.8 71.6 1.0
NH1 A:ARG220 4.3 80.7 1.0
CZ A:ARG220 4.3 81.9 1.0
CD A:LYS226 4.5 69.5 1.0
CE A:LYS226 4.5 70.5 1.0

Chlorine binding site 2 out of 6 in 7ldv

Go back to Chlorine Binding Sites List in 7ldv
Chlorine binding site 2 out of 6 in the Zoogloea Ramigera Biosynthetic Thiolase Q183Y/Y218E/DELH221/S227K Mutant


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Zoogloea Ramigera Biosynthetic Thiolase Q183Y/Y218E/DELH221/S227K Mutant within 5.0Å range:
probe atom residue distance (Å) B Occ
C:Cl404

b:81.8
occ:1.00
 O C:HOH552 3.6 34.2 1.0
O C:ARG41 4.1 57.4 1.0
N C:GLY43 4.1 56.8 1.0
CA C:GLY43 4.1 58.0 1.0
O C:GLU40 4.4 64.9 1.0
C C:ALA42 4.5 54.4 1.0
O C:HOH572 4.6 34.2 1.0
C C:ARG41 4.7 50.5 1.0
O C:ALA42 4.7 55.2 1.0

Chlorine binding site 3 out of 6 in 7ldv

Go back to Chlorine Binding Sites List in 7ldv
Chlorine binding site 3 out of 6 in the Zoogloea Ramigera Biosynthetic Thiolase Q183Y/Y218E/DELH221/S227K Mutant


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 3 of Zoogloea Ramigera Biosynthetic Thiolase Q183Y/Y218E/DELH221/S227K Mutant within 5.0Å range:
probe atom residue distance (Å) B Occ
C:Cl405

b:62.8
occ:1.00
 O C:GLY106 3.3 54.6 1.0
OG C:SER109 3.6 48.7 1.0
N C:ALA108 3.6 51.5 1.0
O1 D:SO4403 3.6 118.9 1.0
C C:ALA108 3.7 46.9 1.0
CA C:ALA108 3.8 48.1 1.0
O C:ALA108 3.8 45.5 1.0
O4 D:SO4403 3.8 118.5 1.0
O2 D:SO4403 4.0 117.3 1.0
C C:ASP107 4.0 49.5 1.0
S D:SO4403 4.0 118.2 1.0
N C:SER109 4.1 46.1 1.0
C C:GLY106 4.1 50.1 1.0
O C:ASP107 4.4 49.0 1.0
CA C:ASP107 4.6 47.8 1.0
CB C:SER109 4.6 44.8 1.0
N C:ASP107 4.7 45.4 1.0
CA C:SER109 4.8 44.2 1.0
CA C:GLY106 5.0 50.4 1.0

Chlorine binding site 4 out of 6 in 7ldv

Go back to Chlorine Binding Sites List in 7ldv
Chlorine binding site 4 out of 6 in the Zoogloea Ramigera Biosynthetic Thiolase Q183Y/Y218E/DELH221/S227K Mutant


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 4 of Zoogloea Ramigera Biosynthetic Thiolase Q183Y/Y218E/DELH221/S227K Mutant within 5.0Å range:
probe atom residue distance (Å) B Occ
C:Cl406

b:67.5
occ:1.00
 NH2 C:ARG41 3.8 58.8 1.0
CD C:PRO201 4.2 32.1 1.0
CG2 C:VAL200 4.4 46.4 1.0
NE C:ARG41 4.5 52.9 1.0
CZ C:ARG41 4.6 57.1 1.0
CA C:VAL200 4.6 42.2 1.0
CB C:VAL200 4.7 45.5 1.0
O C:HOH514 4.8 24.6 1.0
O C:ILE199 4.8 41.0 1.0

Chlorine binding site 5 out of 6 in 7ldv

Go back to Chlorine Binding Sites List in 7ldv
Chlorine binding site 5 out of 6 in the Zoogloea Ramigera Biosynthetic Thiolase Q183Y/Y218E/DELH221/S227K Mutant


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 5 of Zoogloea Ramigera Biosynthetic Thiolase Q183Y/Y218E/DELH221/S227K Mutant within 5.0Å range:
probe atom residue distance (Å) B Occ
C:Cl407

b:60.8
occ:1.00
 NZ C:LYS297 3.7 70.0 1.0
NE C:ARG301 4.2 60.4 1.0
CE C:LYS297 4.2 65.4 1.0
NH2 C:ARG301 4.3 67.3 1.0
CZ C:ARG301 4.7 59.7 1.0
CG C:ARG301 4.9 52.9 1.0

Chlorine binding site 6 out of 6 in 7ldv

Go back to Chlorine Binding Sites List in 7ldv
Chlorine binding site 6 out of 6 in the Zoogloea Ramigera Biosynthetic Thiolase Q183Y/Y218E/DELH221/S227K Mutant


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 6 of Zoogloea Ramigera Biosynthetic Thiolase Q183Y/Y218E/DELH221/S227K Mutant within 5.0Å range:
probe atom residue distance (Å) B Occ
D:Cl406

b:68.0
occ:1.00
 NH2 D:ARG41 3.4 46.6 1.0
NE D:ARG41 3.8 50.9 1.0
CZ D:ARG41 4.0 47.5 1.0
CG2 D:VAL200 4.2 48.2 1.0
CD D:PRO201 4.2 42.7 1.0
CB D:VAL200 4.4 48.3 1.0
CA D:VAL200 4.4 46.4 1.0
O D:ILE199 4.7 42.2 1.0
O D:HOH560 4.8 42.0 1.0
OE2 D:GLU40 5.0 92.3 1.0

Reference:

A.C.Marshall, J.B.Bruning. Engineering Potassium Activation Into Biosynthetic Thiolase. Biochem.J. V. 478 3047 2021.
ISSN: ESSN 1470-8728
PubMed: 34338286
DOI: 10.1042/BCJ20210455
Page generated: Fri Sep 24 12:55:15 2021

Last articles

Zn in 8WB0
Zn in 8WAX
Zn in 8WAU
Zn in 8WAZ
Zn in 8WAY
Zn in 8WAV
Zn in 8WAW
Zn in 8WAT
Zn in 8W7M
Zn in 8WD3
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy