Chlorine in PDB 7m09: Pre-Catalytic Quaternary Complex of Dna Polymerase Lambda with Blunt- Ended Dsb Substrate and Incoming Dumpnpp

Enzymatic activity of Pre-Catalytic Quaternary Complex of Dna Polymerase Lambda with Blunt- Ended Dsb Substrate and Incoming Dumpnpp

All present enzymatic activity of Pre-Catalytic Quaternary Complex of Dna Polymerase Lambda with Blunt- Ended Dsb Substrate and Incoming Dumpnpp:
2.7.7.7;

Protein crystallography data

The structure of Pre-Catalytic Quaternary Complex of Dna Polymerase Lambda with Blunt- Ended Dsb Substrate and Incoming Dumpnpp, PDB code: 7m09 was solved by A.M.Kaminski, K.Bebenek, L.C.Pedersen, T.A.Kunkel, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 39.17 / 1.65
Space group P 21 21 21
Cell size a, b, c (Å), α, β, γ (°) 55.941, 59.615, 140.042, 90, 90, 90
R / Rfree (%) 16.8 / 18.6

Other elements in 7m09:

The structure of Pre-Catalytic Quaternary Complex of Dna Polymerase Lambda with Blunt- Ended Dsb Substrate and Incoming Dumpnpp also contains other interesting chemical elements:

Calcium (Ca) 1 atom
Sodium (Na) 3 atoms
Magnesium (Mg) 2 atoms

Chlorine Binding Sites:

The binding sites of Chlorine atom in the Pre-Catalytic Quaternary Complex of Dna Polymerase Lambda with Blunt- Ended Dsb Substrate and Incoming Dumpnpp (pdb code 7m09). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 2 binding sites of Chlorine where determined in the Pre-Catalytic Quaternary Complex of Dna Polymerase Lambda with Blunt- Ended Dsb Substrate and Incoming Dumpnpp, PDB code: 7m09:
Jump to Chlorine binding site number: 1; 2;

Chlorine binding site 1 out of 2 in 7m09

Go back to Chlorine Binding Sites List in 7m09
Chlorine binding site 1 out of 2 in the Pre-Catalytic Quaternary Complex of Dna Polymerase Lambda with Blunt- Ended Dsb Substrate and Incoming Dumpnpp


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Pre-Catalytic Quaternary Complex of Dna Polymerase Lambda with Blunt- Ended Dsb Substrate and Incoming Dumpnpp within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Cl707

b:47.5
occ:1.00
N A:SER329 3.1 17.4 0.6
N A:SER329 3.1 17.5 0.4
OG A:SER329 3.1 24.0 0.6
NE2 A:GLN270 3.1 18.9 1.0
O A:HOH973 3.6 42.0 1.0
CB A:SER329 3.7 21.8 0.4
CB A:SER329 3.7 21.8 0.6
CA A:ILE328 3.8 22.2 1.0
C A:ILE328 3.9 21.5 1.0
CG2 A:ILE328 4.0 22.2 1.0
CA A:SER329 4.0 18.8 0.6
CA A:SER329 4.0 18.9 0.4
CD A:GLN270 4.0 25.4 1.0
OE1 A:GLN270 4.1 25.9 1.0
CB A:ILE328 4.4 22.2 1.0
NZ A:LYS324 4.5 34.5 1.0
O A:HIS327 4.5 26.9 1.0
CG1 A:ILE328 4.7 26.0 1.0
CE A:LYS324 4.7 42.7 1.0
CB A:ALA266 4.9 19.2 1.0
CG2 A:VAL332 4.9 18.2 1.0
N A:ILE328 5.0 25.8 1.0

Chlorine binding site 2 out of 2 in 7m09

Go back to Chlorine Binding Sites List in 7m09
Chlorine binding site 2 out of 2 in the Pre-Catalytic Quaternary Complex of Dna Polymerase Lambda with Blunt- Ended Dsb Substrate and Incoming Dumpnpp


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Pre-Catalytic Quaternary Complex of Dna Polymerase Lambda with Blunt- Ended Dsb Substrate and Incoming Dumpnpp within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Cl708

b:44.8
occ:1.00
N A:GLU556 3.1 19.7 1.0
O A:HOH836 3.4 36.9 1.0
O A:HOH1039 3.5 27.6 1.0
CA A:THR555 3.6 21.0 1.0
CG A:GLU556 3.8 19.2 1.0
CB A:THR555 3.8 24.3 1.0
C A:THR555 3.8 22.0 1.0
CB A:GLU556 4.0 18.8 1.0
CD2 A:LEU503 4.1 27.0 1.0
CA A:GLU556 4.1 17.8 1.0
O A:HOH1098 4.4 47.7 1.0
OE1 A:GLU556 4.4 21.8 1.0
O A:HOH1101 4.5 39.0 1.0
CD A:GLU556 4.5 20.6 1.0
CG2 A:THR555 4.5 26.2 1.0
O A:PRO554 4.7 25.0 1.0
CB A:ALA500 4.7 19.8 1.0
N A:THR555 4.9 20.3 1.0
CA A:ALA500 4.9 17.1 1.0
OG1 A:THR555 5.0 26.1 1.0

Reference:

A.M.Kaminski, K.Bebenek, T.A.Kunkel, L.C.Pedersen. Comprehensive Structural Survey of Dna Double-Strand Break Synapsis By Dna Polymerase Lambda To Be Published.
Page generated: Tue Jul 30 00:19:03 2024

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