Chlorine in PDB 7m4f: Dna Polymerase Lambda, Dctp:at MG2+ Product State Ternary Complex, 300 Min

Enzymatic activity of Dna Polymerase Lambda, Dctp:at MG2+ Product State Ternary Complex, 300 Min

All present enzymatic activity of Dna Polymerase Lambda, Dctp:at MG2+ Product State Ternary Complex, 300 Min:
2.7.7.7;

Protein crystallography data

The structure of Dna Polymerase Lambda, Dctp:at MG2+ Product State Ternary Complex, 300 Min, PDB code: 7m4f was solved by J.A.Jamsen, S.H.Wilson, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

*Resolution Low / High (Å)*	36.13 / 1.95
*Space group*	P 2₁ 2₁ 2₁
*Cell size a, b, c (Å), α, β, γ (°)*	55.869, 62.065, 142.087, 90, 90, 90
*R / R_free (%)*	20.8 / 24.1

Other elements in 7m4f:

The structure of Dna Polymerase Lambda, Dctp:at MG2+ Product State Ternary Complex, 300 Min also contains other interesting chemical elements:

Sodium	(Na)	3 atoms
Magnesium	(Mg)	1 atom

Chlorine Binding Sites:

The binding sites of Chlorine atom in the Dna Polymerase Lambda, Dctp:at MG2+ Product State Ternary Complex, 300 Min (pdb code 7m4f). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total only one binding site of Chlorine was determined in the Dna Polymerase Lambda, Dctp:at MG2+ Product State Ternary Complex, 300 Min, PDB code: 7m4f:

Chlorine binding site 1 out of 1 in 7m4f

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Chlorine Binding Sites List in 7m4f

Chlorine binding site 1 out of 1 in the Dna Polymerase Lambda, Dctp:at MG2+ Product State Ternary Complex, 300 Min

Mono view

Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Dna Polymerase Lambda, Dctp:at MG2+ Product State Ternary Complex, 300 Min within 5.0Å range:

probe	atom	residue	distance (Å)	B	Occ
A:Cl606 b:81.5 occ:1.00	N	A:GLU556	3.1	47.2	1.0
	CA	A:THR555	3.6	58.0	1.0
	C	A:THR555	3.8	51.1	1.0
	CD1	A:LEU503	3.9	49.4	1.0
	CG	A:GLU556	4.0	46.0	1.0
	CB	A:GLU556	4.1	45.9	1.0
	CA	A:GLU556	4.2	53.4	1.0
	CB	A:THR555	4.2	97.0	1.0
	OE1	A:GLU556	4.6	50.8	1.0
	O	A:PRO554	4.6	59.5	1.0
	CD	A:GLU556	4.7	54.8	1.0
	CB	A:ALA500	4.8	55.2	1.0
	N	A:THR555	4.8	56.0	1.0
	CA	A:ALA500	4.9	49.0	1.0
	CG2	A:THR555	5.0	87.1	1.0
	O	A:THR555	5.0	51.0	1.0

Reference:

J.A.Jamsen, D.D.Shock, S.H.Wilson. Watching Right and Wrong Nucleotide Insertion Captures Hidden Polymerase Fidelity Checkpoints. Nat Commun V. 13 3193 2022.
ISSN: ESSN 2041-1723
PubMed: 35680862
DOI: 10.1038/S41467-022-30141-W

Page generated: Tue Jul 30 00:25:17 2024

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