Chlorine in PDB 7oys: E.Coli'S Putrescine Receptor Variant Potf/D (4JDF) with Mutations E39D Y87S in Complex with Spermidine

The structure of E.Coli'S Putrescine Receptor Variant Potf/D (4JDF) with Mutations E39D Y87S in Complex with Spermidine, PDB code: 7oys was solved by S.Shanmugaratnam, P.Kroeger, B.Hocker, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	39.29 / 1.57
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	37.256, 78.579, 114.435, 90, 90, 90
R / Rfree (%)	17.4 / 20.9

The binding sites of Chlorine atom in the E.Coli'S Putrescine Receptor Variant Potf/D (4JDF) with Mutations E39D Y87S in Complex with Spermidine (pdb code 7oys). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total only one binding site of Chlorine was determined in the E.Coli'S Putrescine Receptor Variant Potf/D (4JDF) with Mutations E39D Y87S in Complex with Spermidine, PDB code: 7oys:

Chlorine binding site 1 out of 1 in the E.Coli'S Putrescine Receptor Variant Potf/D (4JDF) with Mutations E39D Y87S in Complex with Spermidine


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of E.Coli'S Putrescine Receptor Variant Potf/D (4JDF) with Mutations E39D Y87S in Complex with Spermidine within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl414 b:28.6 occ:1.00 HG A:SER87 2.6 27.4 0.5 H A:PHE277 2.6 20.8 1.0 H A:SER87 2.7 28.6 0.5 H A:SER87 2.7 28.6 0.5 HB2 A:ALA86 2.9 29.7 1.0 H A:ALA86 3.1 21.4 1.0 O A:HOH729 3.1 32.9 1.0 HB2 A:SER85 3.2 25.4 1.0 O A:HOH587 3.2 36.0 1.0 HB2 A:TRP276 3.2 23.6 1.0 O A:HOH670 3.3 22.9 1.0 OG A:SER87 3.4 22.9 0.5 N A:SER87 3.4 23.9 1.0 HD1 A:PHE277 3.5 33.6 1.0 O A:PHE277 3.5 18.6 1.0 N A:PHE277 3.5 17.3 1.0 HA A:TRP276 3.5 18.5 1.0 N A:ALA86 3.6 17.9 1.0 HB3 A:SER87 3.6 28.8 0.5 HB3 A:TRP276 3.7 23.6 1.0 CB A:ALA86 3.7 24.7 1.0 OG A:SER87 3.8 23.6 0.5 HB2 A:SER87 3.8 28.8 0.5 CB A:TRP276 3.8 19.7 1.0 O A:HOH532 3.9 21.3 1.0 HG A:SER87 3.9 28.3 0.5 CA A:ALA86 4.0 18.7 1.0 CB A:SER87 4.0 24.0 0.5 CB A:SER87 4.0 24.0 0.5 CA A:TRP276 4.1 15.4 1.0 HB1 A:ALA86 4.1 29.7 1.0 CB A:SER85 4.1 21.2 1.0 C A:ALA86 4.2 24.4 1.0 HB2 A:PHE277 4.2 22.4 1.0 C A:PHE277 4.3 18.3 1.0 CD1 A:PHE277 4.3 28.0 1.0 C A:TRP276 4.3 14.3 1.0 CA A:SER87 4.3 28.3 0.5 CA A:SER87 4.3 28.4 0.5 CA A:PHE277 4.3 16.9 1.0 C A:SER85 4.4 20.6 1.0 HA A:SER85 4.4 23.7 1.0 HB3 A:ALA86 4.5 29.7 1.0 CA A:SER85 4.6 19.8 1.0 HD2 A:HIS123 4.6 34.1 1.0 HB3 A:SER85 4.6 25.4 1.0 CB A:PHE277 4.7 18.7 1.0 HA A:SER87 4.7 34.0 0.5 HA A:SER87 4.7 34.1 0.5 HB3 A:GLN348 4.8 27.5 1.0 OG A:SER85 4.8 22.7 1.0 NE2 A:HIS123 4.8 30.8 1.0 HG A:SER85 4.8 27.2 1.0 CD2 A:HIS123 4.9 28.4 1.0 HB3 A:SER87 4.9 28.8 0.5 CG A:PHE277 4.9 21.6 1.0 HB2 A:SER87 4.9 28.8 0.5 HA A:ALA86 5.0 22.4 1.0

P.Kroger, S.Shanmugaratnam, U.Scheib, B.Hocker. Fine-Tuning Spermidine Binding Modes in the Putrescine Binding Protein Potf. J.Biol.Chem. 01419 2021.
ISSN: ESSN 1083-351X
PubMed: 34801550
DOI: 10.1016/J.JBC.2021.101419