Chlorine in PDB 7oyt: E.Coli'S Putrescine Receptor Variant Potf/D (4JDF) with Mutations E39D F88L in Complex with Spermidine

The structure of E.Coli'S Putrescine Receptor Variant Potf/D (4JDF) with Mutations E39D F88L in Complex with Spermidine, PDB code: 7oyt was solved by S.Shanmugaratnam, P.Kroeger, B.Hocker, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	39.01 / 1.60
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	37.135, 78.028, 118.444, 90, 90, 90
R / Rfree (%)	17.8 / 20.9

The binding sites of Chlorine atom in the E.Coli'S Putrescine Receptor Variant Potf/D (4JDF) with Mutations E39D F88L in Complex with Spermidine (pdb code 7oyt). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 2 binding sites of Chlorine where determined in the E.Coli'S Putrescine Receptor Variant Potf/D (4JDF) with Mutations E39D F88L in Complex with Spermidine, PDB code: 7oyt:
Jump to Chlorine binding site number: 1; 2;

Chlorine binding site 1 out of 2 in the E.Coli'S Putrescine Receptor Variant Potf/D (4JDF) with Mutations E39D F88L in Complex with Spermidine


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of E.Coli'S Putrescine Receptor Variant Potf/D (4JDF) with Mutations E39D F88L in Complex with Spermidine within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl425 b:19.4 occ:0.61 CL A:CL425 0.0 19.4 0.6 CL A:CL425 1.2 28.8 0.4 O A:HOH530 3.2 32.7 1.0 N A:TYR87 3.2 25.6 1.0 O A:HOH627 3.3 34.5 1.0 N A:PHE277 3.4 18.5 1.0 O A:PHE277 3.4 24.4 1.0 N A:ALA86 3.5 22.3 1.0 O A:HOH615 3.6 43.1 1.0 CB A:TYR87 3.7 27.2 1.0 CB A:TRP276 3.7 17.5 1.0 CB A:SER85 3.8 25.4 1.0 CB A:ALA86 3.9 19.4 1.0 O A:HOH542 3.9 26.3 1.0 CA A:ALA86 4.0 21.0 1.0 CA A:TRP276 4.0 16.9 1.0 C A:ALA86 4.1 28.5 1.0 CA A:TYR87 4.1 28.7 1.0 C A:SER85 4.2 25.3 1.0 C A:TRP276 4.2 18.3 1.0 C A:PHE277 4.3 21.4 1.0 CD2 A:PHE277 4.3 24.0 1.0 CA A:PHE277 4.3 22.8 1.0 OG A:SER85 4.4 30.3 1.0 CA A:SER85 4.4 26.1 1.0 NE2 A:HIS123 4.6 30.1 1.0 CD2 A:HIS123 4.7 30.5 1.0 CB A:PHE277 4.8 21.2 1.0

Chlorine binding site 2 out of 2 in the E.Coli'S Putrescine Receptor Variant Potf/D (4JDF) with Mutations E39D F88L in Complex with Spermidine


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of E.Coli'S Putrescine Receptor Variant Potf/D (4JDF) with Mutations E39D F88L in Complex with Spermidine within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl425 b:28.8 occ:0.39 CL A:CL425 0.0 28.8 0.4 CL A:CL425 1.2 19.4 0.6 O A:HOH530 2.3 32.7 1.0 N A:PHE277 3.2 18.5 1.0 CD2 A:PHE277 3.3 24.0 1.0 O A:HOH542 3.4 26.3 1.0 CA A:TRP276 3.7 16.9 1.0 CB A:ALA86 3.7 19.4 1.0 N A:TYR87 3.8 25.6 1.0 O A:PHE277 3.8 24.4 1.0 CB A:TRP276 3.9 17.5 1.0 C A:TRP276 3.9 18.3 1.0 CE2 A:PHE277 4.0 29.2 1.0 O A:HOH615 4.0 43.1 1.0 NE2 A:HIS123 4.0 30.1 1.0 N A:ALA86 4.1 22.3 1.0 CD2 A:HIS123 4.1 30.5 1.0 CA A:PHE277 4.1 22.8 1.0 O A:HOH627 4.2 34.5 1.0 CG A:PHE277 4.2 21.6 1.0 CB A:PHE277 4.2 21.2 1.0 CA A:ALA86 4.3 21.0 1.0 CB A:TYR87 4.4 27.2 1.0 C A:ALA86 4.4 28.5 1.0 C A:PHE277 4.4 21.4 1.0 CA A:TYR87 4.6 28.7 1.0 O A:ALA275 4.8 21.0 1.0 N A:TRP276 4.9 16.9 1.0 CB A:SER85 5.0 25.4 1.0

P.Kroger, S.Shanmugaratnam, U.Scheib, B.Hocker. Fine-Tuning Spermidine Binding Modes in the Putrescine Binding Protein Potf. J.Biol.Chem. 01419 2021.
ISSN: ESSN 1083-351X
PubMed: 34801550
DOI: 10.1016/J.JBC.2021.101419