Chlorine in PDB 7oyv: E.Coli'S Putrescine Receptor Variant Potf/D (4JDF) with Mutations E39D F88A S247D in Complex with Spermidine

The structure of E.Coli'S Putrescine Receptor Variant Potf/D (4JDF) with Mutations E39D F88A S247D in Complex with Spermidine, PDB code: 7oyv was solved by S.Shanmugaratnam, P.Kroeger, B.Hocker, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	47.58 / 1.90
Space group	C 2 2 21
Cell size a, b, c (Å), α, β, γ (°)	73.613, 122.576, 190.32, 90, 90, 90
R / Rfree (%)	22.7 / 27.6

The binding sites of Chlorine atom in the E.Coli'S Putrescine Receptor Variant Potf/D (4JDF) with Mutations E39D F88A S247D in Complex with Spermidine (pdb code 7oyv). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 2 binding sites of Chlorine where determined in the E.Coli'S Putrescine Receptor Variant Potf/D (4JDF) with Mutations E39D F88A S247D in Complex with Spermidine, PDB code: 7oyv:
Jump to Chlorine binding site number: 1; 2;

Chlorine binding site 1 out of 2 in the E.Coli'S Putrescine Receptor Variant Potf/D (4JDF) with Mutations E39D F88A S247D in Complex with Spermidine


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of E.Coli'S Putrescine Receptor Variant Potf/D (4JDF) with Mutations E39D F88A S247D in Complex with Spermidine within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl507 b:34.8 occ:1.00 O A:HOH660 2.7 32.9 1.0 N A:PHE277 3.3 33.9 1.0 N A:TYR87 3.4 35.0 1.0 N A:ALA86 3.4 32.0 1.0 CB A:ALA86 3.5 36.0 1.0 O A:PHE277 3.6 34.3 1.0 CD2 A:TYR87 3.7 32.1 1.0 CA A:TRP276 3.8 33.5 1.0 CB A:TRP276 3.8 33.8 1.0 CA A:ALA86 3.8 34.2 1.0 CD2 A:PHE277 4.0 40.5 1.0 C A:ALA86 4.0 37.2 1.0 C A:TRP276 4.1 34.7 1.0 CB A:TYR87 4.1 36.1 1.0 CB A:SER85 4.3 27.9 1.0 CA A:PHE277 4.3 33.4 1.0 C A:PHE277 4.3 34.4 1.0 C A:SER85 4.3 32.4 1.0 CA A:TYR87 4.3 37.3 1.0 CG A:TYR87 4.4 33.3 1.0 CB A:PHE277 4.5 33.8 1.0 CA A:SER85 4.7 30.6 1.0 CE2 A:TYR87 4.7 35.0 1.0 CG A:PHE277 4.7 36.9 1.0 NE2 A:HIS123 4.7 43.1 1.0 CD2 A:HIS123 4.8 44.2 1.0 CE2 A:PHE277 4.9 43.0 1.0 O A:HOH645 4.9 31.6 1.0 O A:ALA275 4.9 30.4 1.0

Chlorine binding site 2 out of 2 in the E.Coli'S Putrescine Receptor Variant Potf/D (4JDF) with Mutations E39D F88A S247D in Complex with Spermidine


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of E.Coli'S Putrescine Receptor Variant Potf/D (4JDF) with Mutations E39D F88A S247D in Complex with Spermidine within 5.0Å range: probe atom residue distance (Å) B Occ B:Cl506 b:31.5 occ:1.00 O B:HOH654 3.3 30.3 1.0 N B:PHE277 3.3 37.9 1.0 N B:TYR87 3.3 32.3 1.0 N B:ALA86 3.6 27.0 1.0 CA B:TRP276 3.6 31.2 1.0 CB B:ALA86 3.6 31.1 1.0 CB B:TRP276 3.7 30.3 1.0 O B:PHE277 3.7 35.7 1.0 CD2 B:TYR87 3.8 32.3 1.0 CA B:ALA86 3.9 30.7 1.0 C B:TRP276 4.0 35.1 1.0 CB B:TYR87 4.0 30.4 1.0 C B:ALA86 4.1 30.2 1.0 CA B:TYR87 4.3 31.6 1.0 CA B:PHE277 4.3 36.3 1.0 CB B:SER85 4.3 30.5 1.0 CG B:TYR87 4.4 32.1 1.0 C B:PHE277 4.5 34.5 1.0 NE2 B:HIS123 4.5 37.2 1.0 CD2 B:PHE277 4.5 50.8 1.0 C B:SER85 4.5 30.8 1.0 O B:ALA275 4.7 32.9 1.0 CB B:PHE277 4.7 38.7 1.0 O B:HOH656 4.8 25.2 1.0 CE2 B:TYR87 4.8 31.4 1.0 N B:TRP276 4.9 29.1 1.0 CA B:SER85 4.9 30.3 1.0 CD2 B:HIS123 5.0 34.2 1.0

P.Kroger, S.Shanmugaratnam, U.Scheib, B.Hocker. Fine-Tuning Spermidine Binding Modes in the Putrescine Binding Protein Potf. J.Biol.Chem. 01419 2021.
ISSN: ESSN 1083-351X
PubMed: 34801550
DOI: 10.1016/J.JBC.2021.101419