Chlorine in PDB 7pfj: 20 Minute FE2+ Soaked Structure of Synftn Variant E141A

All present enzymatic activity of 20 Minute FE2+ Soaked Structure of Synftn Variant E141A:
1.16.3.2;

The structure of 20 Minute FE2+ Soaked Structure of Synftn Variant E141A, PDB code: 7pfj was solved by A.M.Hemmings, J.M.Bradley, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	40.53 / 1.65
Space group	F 4 3 2
Cell size a, b, c (Å), α, β, γ (°)	176.67, 176.67, 176.67, 90, 90, 90
R / Rfree (%)	15 / 17.4

The structure of 20 Minute FE2+ Soaked Structure of Synftn Variant E141A also contains other interesting chemical elements:

Sodium	(Na)	2 atoms
Iron	(Fe)	3 atoms

The binding sites of Chlorine atom in the 20 Minute FE2+ Soaked Structure of Synftn Variant E141A (pdb code 7pfj). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 2 binding sites of Chlorine where determined in the 20 Minute FE2+ Soaked Structure of Synftn Variant E141A, PDB code: 7pfj:
Jump to Chlorine binding site number: 1; 2;

Chlorine binding site 1 out of 2 in the 20 Minute FE2+ Soaked Structure of Synftn Variant E141A


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of 20 Minute FE2+ Soaked Structure of Synftn Variant E141A within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl204 b:66.5 occ:1.00 NH1 A:ARG93 3.3 36.4 1.0 O A:HOH517 3.4 40.1 1.0 CD A:ARG93 3.6 33.4 1.0 CG2 A:THR105 3.9 29.1 1.0 OG1 A:THR105 4.2 29.2 1.0 CZ A:ARG93 4.3 35.8 1.0 NE A:ARG93 4.4 34.6 1.0 CB A:THR105 4.7 27.9 1.0 CG A:ARG93 4.8 31.4 1.0 CB A:ARG93 4.8 30.1 1.0

Chlorine binding site 2 out of 2 in the 20 Minute FE2+ Soaked Structure of Synftn Variant E141A


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of 20 Minute FE2+ Soaked Structure of Synftn Variant E141A within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl205 b:33.5 occ:1.00 O A:HOH356 3.0 34.2 1.0 O A:HOH494 3.1 50.6 1.0 N A:ALA100 3.3 28.2 1.0 CB A:ALA100 3.7 28.0 1.0 N A:LEU99 3.8 26.8 1.0 CB A:SER98 3.9 31.1 1.0 CD1 A:LEU167 3.9 27.1 1.0 CA A:ALA100 4.0 28.8 1.0 CB A:LEU99 4.1 27.2 1.0 C A:LEU99 4.2 27.6 1.0 CA A:GLY162 4.2 34.0 1.0 CA A:LEU99 4.2 26.9 1.0 OG A:SER98 4.2 31.6 1.0 CG1 A:VAL157 4.4 27.0 1.0 N A:GLY162 4.5 33.6 1.0 C A:SER98 4.6 29.2 1.0 O A:HOH486 4.7 39.2 1.0 CA A:SER98 4.7 31.1 1.0 CD2 A:LEU167 4.8 29.5 1.0 CG A:LEU167 5.0 27.8 1.0

J.M.Bradley, J.Fair, A.M.Hemmings, N.E.Le Brun. Key Carboxylate Residues For Iron Transit Through the Prokaryotic Ferritin Syn Ftn. Microbiology (Reading, V. 167 2021ENGL.).
ISSN: ESSN 1465-2080
PubMed: 34825885
DOI: 10.1099/MIC.0.001105