Chlorine in PDB 7r63: NB82, A Nanobody Against Voltage Gated Sodium Channels NAV1.4 and NAV1.5

The structure of NB82, A Nanobody Against Voltage Gated Sodium Channels NAV1.4 and NAV1.5, PDB code: 7r63 was solved by L.Srinivasan, S.B.Gabelli, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	29.65 / 2.00
Space group	C 2 2 21
Cell size a, b, c (Å), α, β, γ (°)	77.745, 82.474, 170.7, 90, 90, 90
R / Rfree (%)	18.4 / 24.4

The structure of NB82, A Nanobody Against Voltage Gated Sodium Channels NAV1.4 and NAV1.5 also contains other interesting chemical elements:

Sodium

(Na)

4 atoms

The binding sites of Chlorine atom in the NB82, A Nanobody Against Voltage Gated Sodium Channels NAV1.4 and NAV1.5 (pdb code 7r63). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total only one binding site of Chlorine was determined in the NB82, A Nanobody Against Voltage Gated Sodium Channels NAV1.4 and NAV1.5, PDB code: 7r63:

Chlorine binding site 1 out of 1 in the NB82, A Nanobody Against Voltage Gated Sodium Channels NAV1.4 and NAV1.5


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of NB82, A Nanobody Against Voltage Gated Sodium Channels NAV1.4 and NAV1.5 within 5.0Å range: probe atom residue distance (Å) B Occ C:Cl203 b:57.8 occ:1.00 N C:GLY42 3.5 28.9 1.0 CA C:PRO41 3.7 30.6 1.0 C C:PRO41 4.1 30.0 1.0 O C:HOH342 4.3 38.5 1.0 CB C:PRO41 4.4 32.2 1.0 CA C:GLY42 4.6 31.0 1.0 O C:ALA40 4.7 27.8 1.0 N C:PRO41 4.7 29.6 1.0

L.Srinivasan, V.Alzogaray, D.Selvakumar, S.Nathan, J.B.Yoder, K.M.Wright, S.Klinke, J.N.Nwafor, M.S.Labanda, F.A.Goldbaum, A.Schon, E.Freire, G.F.Tomaselli, L.M.Amzel, M.Ben-Johny, S.B.Gabelli. Development of High-Affinity Nanobodies Specific For Na V 1.4 and Na V 1.5 Voltage-Gated Sodium Channel Isoforms. J.Biol.Chem. V. 298 01763 2022.
ISSN: ESSN 1083-351X
PubMed: 35202650
DOI: 10.1016/J.JBC.2022.101763