Chlorine in PDB 7sak: Crystal Structure of LAM4 Nanobody Bound to Mcherry

The structure of Crystal Structure of LAM4 Nanobody Bound to Mcherry, PDB code: 7sak was solved by A.T.Q.Cong, M.J.Schellenberg, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	42.15 / 1.15
Space group	C 1 2 1
Cell size a, b, c (Å), α, β, γ (°)	156.554, 41.92, 47.827, 90, 94.37, 90
R / Rfree (%)	11.7 / 14.4

The binding sites of Chlorine atom in the Crystal Structure of LAM4 Nanobody Bound to Mcherry (pdb code 7sak). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total only one binding site of Chlorine was determined in the Crystal Structure of LAM4 Nanobody Bound to Mcherry, PDB code: 7sak:

Chlorine binding site 1 out of 1 in the Crystal Structure of LAM4 Nanobody Bound to Mcherry


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Crystal Structure of LAM4 Nanobody Bound to Mcherry within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl302 b:12.6 occ:1.00 H A:ASP154 2.3 15.0 1.0 O A:HOH408 3.1 36.2 1.0 N A:ASP154 3.2 12.5 1.0 HA A:GLU153 3.2 11.4 1.0 HA A:ASP154 3.6 16.3 1.0 HG3 A:GLU153 3.7 15.7 1.0 OD1 A:ASP154 3.7 19.7 0.3 O A:HOH547 3.9 44.8 1.0 CA A:ASP154 4.0 14.4 0.7 CA A:ASP154 4.0 13.6 0.3 CA A:GLU153 4.0 9.5 1.0 C A:GLU153 4.1 9.8 1.0 HB2 A:ASP154 4.3 22.8 0.7 CG A:GLU153 4.4 13.1 1.0 CG A:ASP154 4.5 17.7 0.3 HG2 A:GLU153 4.6 15.7 1.0 H A:GLY155 4.6 13.8 1.0 CB A:GLU153 4.7 11.3 1.0 CB A:ASP154 4.7 19.0 0.7 CB A:ASP154 4.8 16.3 0.3 O A:HOH470 4.9 26.0 1.0 HB2 A:GLU153 5.0 13.6 1.0

A.T.Q.Cong, T.L.Witter, M.J.Schellenberg. High-Efficiency Recombinant Protein Purification Using Mcherry and Yfp Nanobody Affinity Matrices. Protein Sci. V. 31 E4383 2022.
ISSN: ESSN 1469-896X
PubMed: 36040252
DOI: 10.1002/PRO.4383