Chlorine in PDB 7t59: Structure of Mouse Double Minute 2 Homolog (MDM2) Ring Domain

All present enzymatic activity of Structure of Mouse Double Minute 2 Homolog (MDM2) Ring Domain:
2.3.2.27;

The structure of Structure of Mouse Double Minute 2 Homolog (MDM2) Ring Domain, PDB code: 7t59 was solved by M.Luo, J.R.Terrell, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	31.44 / 1.48
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	29.142, 39.746, 103.014, 90, 94.02, 90
R / Rfree (%)	14.7 / 19.8

The structure of Structure of Mouse Double Minute 2 Homolog (MDM2) Ring Domain also contains other interesting chemical elements:

Zinc

(Zn)

8 atoms

The binding sites of Chlorine atom in the Structure of Mouse Double Minute 2 Homolog (MDM2) Ring Domain (pdb code 7t59). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 4 binding sites of Chlorine where determined in the Structure of Mouse Double Minute 2 Homolog (MDM2) Ring Domain, PDB code: 7t59:
Jump to Chlorine binding site number: 1; 2; 3; 4;

Chlorine binding site 1 out of 4 in the Structure of Mouse Double Minute 2 Homolog (MDM2) Ring Domain


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Structure of Mouse Double Minute 2 Homolog (MDM2) Ring Domain within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl503 b:19.1 occ:1.00 O A:HOH635 3.0 21.6 1.0 N A:ILE485 3.1 9.7 1.0 NZ B:LYS454 3.2 17.1 1.0 CA A:MET484 3.8 11.1 1.0 CE B:LYS454 3.9 17.9 1.0 O A:ILE485 3.9 13.3 1.0 C A:MET484 4.0 12.4 1.0 CA A:ILE485 4.1 10.6 1.0 CB A:ILE485 4.1 8.8 1.0 CB A:MET484 4.1 11.4 1.0 CG A:LYS469 4.2 21.7 1.0 CG1 A:ILE485 4.2 10.4 1.0 CD A:LYS466 4.3 16.9 1.0 CE A:LYS466 4.3 21.5 1.0 CD B:LYS454 4.3 16.6 1.0 C A:ILE485 4.5 11.8 1.0 CE A:LYS469 4.5 25.1 1.0 CD A:LYS469 4.8 24.4 1.0 O A:HOH641 4.9 26.9 1.0

Chlorine binding site 2 out of 4 in the Structure of Mouse Double Minute 2 Homolog (MDM2) Ring Domain


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Structure of Mouse Double Minute 2 Homolog (MDM2) Ring Domain within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl504 b:15.3 occ:1.00 O B:HOH636 3.2 27.7 1.0 O A:HOH649 3.3 15.4 1.0 O B:HOH647 3.3 34.2 1.0 CD1 A:TYR489 4.1 13.1 1.0 CE1 A:TYR489 4.2 15.1 1.0 O B:HOH639 4.8 26.5 1.0 CB B:GLU427 4.8 20.4 1.0 O A:HOH629 4.9 11.5 1.0 O A:HOH606 4.9 22.3 1.0

Chlorine binding site 3 out of 4 in the Structure of Mouse Double Minute 2 Homolog (MDM2) Ring Domain


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 3 of Structure of Mouse Double Minute 2 Homolog (MDM2) Ring Domain within 5.0Å range: probe atom residue distance (Å) B Occ C:Cl503 b:22.2 occ:1.00 O C:HOH632 2.9 21.0 1.0 N C:ILE485 3.2 12.3 1.0 NZ D:LYS454 3.2 21.6 1.0 CA C:MET484 3.8 11.6 1.0 O C:ILE485 4.0 16.8 1.0 C C:MET484 4.0 12.0 1.0 CB C:MET484 4.1 14.1 1.0 CE D:LYS454 4.1 20.3 1.0 CB C:ILE485 4.1 11.6 1.0 CA C:ILE485 4.1 11.1 1.0 CG1 C:ILE485 4.3 14.5 1.0 CD C:LYS466 4.3 17.5 1.0 CG C:LYS469 4.3 28.5 1.0 CD D:LYS454 4.3 17.3 1.0 CE C:LYS466 4.4 21.3 1.0 C C:ILE485 4.5 13.0 1.0 CE C:LYS469 4.6 34.2 1.0 CD C:LYS469 4.8 30.1 1.0 O C:HOH637 4.9 24.4 1.0

Chlorine binding site 4 out of 4 in the Structure of Mouse Double Minute 2 Homolog (MDM2) Ring Domain


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 4 of Structure of Mouse Double Minute 2 Homolog (MDM2) Ring Domain within 5.0Å range: probe atom residue distance (Å) B Occ C:Cl504 b:18.4 occ:1.00 O D:HOH633 3.2 28.9 1.0 O C:HOH647 3.2 17.3 1.0 O C:HOH654 3.3 34.6 1.0 CD2 C:TYR489 4.1 13.9 1.0 CE2 C:TYR489 4.2 16.3 1.0 O D:HOH644 4.8 29.6 1.0 CB D:GLU427 4.9 21.7 1.0 O C:HOH631 4.9 13.8 1.0 O D:HOH628 5.0 30.9 1.0 O C:HOH605 5.0 27.0 1.0

J.R.Terrell, S.Tang, O.O.Faniyi, I.H.Jeong, J.Yin, B.Nijampatnam, S.E.Velu, W.Wang, R.Zhang, M.Luo. Structural Studies of Antitumor Compounds That Target the Ring Domain of MDM2. Protein Sci. V. 31 E4367 2022.
ISSN: ESSN 1469-896X
PubMed: 35900024
DOI: 10.1002/PRO.4367