Chlorine in PDB 7xe9: T4 Lysozyme Mutant-S44C/C54T/N68C/A93C/C97A/T115C, Dmso 20%

All present enzymatic activity of T4 Lysozyme Mutant-S44C/C54T/N68C/A93C/C97A/T115C, Dmso 20%:
3.2.1.17;

The structure of T4 Lysozyme Mutant-S44C/C54T/N68C/A93C/C97A/T115C, Dmso 20%, PDB code: 7xe9 was solved by T.Tamada, T.Hiromoto, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	25.93 / 1.50
Space group	P 32 2 1
Cell size a, b, c (Å), α, β, γ (°)	59.873, 59.873, 96.03, 90, 90, 120
R / Rfree (%)	20.2 / 23.2

The structure of T4 Lysozyme Mutant-S44C/C54T/N68C/A93C/C97A/T115C, Dmso 20% also contains other interesting chemical elements:

Sodium

(Na)

1 atom

The binding sites of Chlorine atom in the T4 Lysozyme Mutant-S44C/C54T/N68C/A93C/C97A/T115C, Dmso 20% (pdb code 7xe9). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total only one binding site of Chlorine was determined in the T4 Lysozyme Mutant-S44C/C54T/N68C/A93C/C97A/T115C, Dmso 20%, PDB code: 7xe9:

Chlorine binding site 1 out of 1 in the T4 Lysozyme Mutant-S44C/C54T/N68C/A93C/C97A/T115C, Dmso 20%


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of T4 Lysozyme Mutant-S44C/C54T/N68C/A93C/C97A/T115C, Dmso 20% within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl209 b:28.1 occ:1.00 O A:HOH443 3.3 35.5 1.0 N A:ARG145 3.3 20.3 1.0 N A:ASN144 3.3 19.2 1.0 C A:THR142 3.3 21.6 1.0 CA A:THR142 3.4 19.2 1.0 CB A:THR142 3.6 20.3 1.0 O A:THR142 3.7 21.0 1.0 N A:PRO143 3.7 21.5 1.0 CB A:ASN144 3.7 22.8 1.0 CA A:ASN144 3.9 20.7 1.0 CB A:ARG145 4.0 20.0 1.0 C A:ASN144 4.1 21.1 1.0 CD A:PRO143 4.1 22.0 1.0 C A:PRO143 4.2 20.7 1.0 CG2 A:THR142 4.2 20.5 1.0 CA A:ARG145 4.2 20.3 1.0 CA A:PRO143 4.5 17.7 1.0 O A:HOH320 4.7 31.9 1.0 CG A:ASN144 4.7 26.1 1.0 OG1 A:THR142 4.8 21.5 1.0 N A:THR142 4.8 20.9 1.0 CG A:PRO143 4.9 22.1 1.0

T.Hiromoto, T.Ikura, E.Honjo, M.Blaber, R.Kuroki, T.Tamada. Creation of Cross-Linked Crystals with Intermolecular Disulfide Bonds Connecting Symmetry-Related Molecules Allows Retention of Tertiary Structure in Different Solvent Conditions. Front Mol Biosci V. 9 08394 2022.
ISSN: ESSN 2296-889X
PubMed: 35755825
DOI: 10.3389/FMOLB.2022.908394