Chlorine in PDB 7zd7: Crystal Structure of the R24E/E352T Double Mutant of S-Adenosyl-L- Homocysteine Hydrolase From Synechocystis Sp. Pcc 6803 Cocrystallized with Adenosine in the Presence of Rb+ Cations

Enzymatic activity of Crystal Structure of the R24E/E352T Double Mutant of S-Adenosyl-L- Homocysteine Hydrolase From Synechocystis Sp. Pcc 6803 Cocrystallized with Adenosine in the Presence of Rb+ Cations

All present enzymatic activity of Crystal Structure of the R24E/E352T Double Mutant of S-Adenosyl-L- Homocysteine Hydrolase From Synechocystis Sp. Pcc 6803 Cocrystallized with Adenosine in the Presence of Rb+ Cations:
3.3.1.1;

Protein crystallography data

The structure of Crystal Structure of the R24E/E352T Double Mutant of S-Adenosyl-L- Homocysteine Hydrolase From Synechocystis Sp. Pcc 6803 Cocrystallized with Adenosine in the Presence of Rb+ Cations, PDB code: 7zd7 was solved by P.H.Malecki, B.Imiolczyk, K.Wozniak, K.Brzezinski, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 51.51 / 1.90
Space group C 2 2 21
Cell size a, b, c (Å), α, β, γ (°) 120.897, 196.825, 81.92, 90, 90, 90
R / Rfree (%) 16.6 / 20

Other elements in 7zd7:

The structure of Crystal Structure of the R24E/E352T Double Mutant of S-Adenosyl-L- Homocysteine Hydrolase From Synechocystis Sp. Pcc 6803 Cocrystallized with Adenosine in the Presence of Rb+ Cations also contains other interesting chemical elements:

Rubidium (Rb) 3 atoms

Chlorine Binding Sites:

The binding sites of Chlorine atom in the Crystal Structure of the R24E/E352T Double Mutant of S-Adenosyl-L- Homocysteine Hydrolase From Synechocystis Sp. Pcc 6803 Cocrystallized with Adenosine in the Presence of Rb+ Cations (pdb code 7zd7). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 3 binding sites of Chlorine where determined in the Crystal Structure of the R24E/E352T Double Mutant of S-Adenosyl-L- Homocysteine Hydrolase From Synechocystis Sp. Pcc 6803 Cocrystallized with Adenosine in the Presence of Rb+ Cations, PDB code: 7zd7:
Jump to Chlorine binding site number: 1; 2; 3;

Chlorine binding site 1 out of 3 in 7zd7

Go back to Chlorine Binding Sites List in 7zd7
Chlorine binding site 1 out of 3 in the Crystal Structure of the R24E/E352T Double Mutant of S-Adenosyl-L- Homocysteine Hydrolase From Synechocystis Sp. Pcc 6803 Cocrystallized with Adenosine in the Presence of Rb+ Cations


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Crystal Structure of the R24E/E352T Double Mutant of S-Adenosyl-L- Homocysteine Hydrolase From Synechocystis Sp. Pcc 6803 Cocrystallized with Adenosine in the Presence of Rb+ Cations within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Cl507

b:49.0
occ:0.50
 CL A:CL507 0.0 49.0 0.5
CL A:CL507 2.1 44.7 0.5
NH2 A:ARG41 3.5 38.9 1.0
NE A:ARG41 3.6 44.8 1.0
CZ A:ARG41 4.0 43.9 1.0
CD2 A:LEU368 4.3 34.8 1.0
CG1 A:ILE38 4.5 33.1 1.0
CD1 A:LEU368 4.6 33.7 1.0
CD A:ARG41 4.7 45.0 1.0
O A:HOH858 4.8 63.5 1.0
CD1 A:LEU398 4.9 34.9 1.0
CG A:GLU394 5.0 35.6 1.0

Chlorine binding site 2 out of 3 in 7zd7

Go back to Chlorine Binding Sites List in 7zd7
Chlorine binding site 2 out of 3 in the Crystal Structure of the R24E/E352T Double Mutant of S-Adenosyl-L- Homocysteine Hydrolase From Synechocystis Sp. Pcc 6803 Cocrystallized with Adenosine in the Presence of Rb+ Cations


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Crystal Structure of the R24E/E352T Double Mutant of S-Adenosyl-L- Homocysteine Hydrolase From Synechocystis Sp. Pcc 6803 Cocrystallized with Adenosine in the Presence of Rb+ Cations within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Cl507

b:44.7
occ:0.50
 CL A:CL507 0.0 44.7 0.5
CL A:CL507 2.1 49.0 0.5
NE A:ARG41 3.2 44.8 1.0
CD A:ARG41 3.9 45.0 1.0
NH2 A:ARG41 4.0 38.9 1.0
CZ A:ARG41 4.0 43.9 1.0
CG1 A:ILE38 4.1 33.1 1.0
CA A:ILE38 4.2 37.3 1.0
O A:GLN37 4.3 39.7 1.0
N A:ILE38 4.4 35.0 1.0
CB A:ARG41 4.4 40.2 1.0
C A:GLN37 4.5 39.7 1.0
CB A:ILE38 4.8 34.5 1.0
CG A:ARG41 4.8 43.9 1.0
CB A:GLN37 4.9 42.0 1.0
O A:HOH715 4.9 44.6 1.0
CD1 A:LEU398 5.0 34.9 1.0

Chlorine binding site 3 out of 3 in 7zd7

Go back to Chlorine Binding Sites List in 7zd7
Chlorine binding site 3 out of 3 in the Crystal Structure of the R24E/E352T Double Mutant of S-Adenosyl-L- Homocysteine Hydrolase From Synechocystis Sp. Pcc 6803 Cocrystallized with Adenosine in the Presence of Rb+ Cations


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 3 of Crystal Structure of the R24E/E352T Double Mutant of S-Adenosyl-L- Homocysteine Hydrolase From Synechocystis Sp. Pcc 6803 Cocrystallized with Adenosine in the Presence of Rb+ Cations within 5.0Å range:
probe atom residue distance (Å) B Occ
C:Cl506

b:70.4
occ:1.00
 NE C:ARG41 3.6 43.0 1.0
NH2 C:ARG41 3.8 43.4 1.0
O C:HOH644 3.8 52.5 1.0
CG1 C:ILE38 4.1 40.2 1.0
CZ C:ARG41 4.2 43.3 1.0
O C:HOH823 4.5 62.1 1.0
O C:HOH822 4.5 60.0 1.0
CD1 C:LEU398 4.5 36.8 1.0
NE2 C:GLN37 4.6 40.5 0.4
CD C:ARG41 4.6 47.5 1.0
CD2 C:LEU368 4.9 41.3 1.0
CD C:GLN37 5.0 43.3 0.4
CA C:ILE38 5.0 41.0 1.0

Reference:

P.H.Malecki, B.Imiolczyk, J.Barciszewski, J.Czyrko-Horczak, J.Sliwiak, M.Gawel, K.Wozniak, M.Jaskolski, K.Brzezinski. Biochemical and Structural Insights Into An Unusual, Alkali-Metal-Independent S-Adenosyl-L-Homocysteine Hydrolase From Synechocystis Sp. Pcc 6803. Acta Crystallogr D Struct V. 78 865 2022BIOL.
ISSN: ISSN 2059-7983
PubMed: 35775986
DOI: 10.1107/S2059798322005605
Page generated: Tue Jul 30 06:12:17 2024

Last articles

Zn in 9JPJ
Zn in 9JP7
Zn in 9JPK
Zn in 9JPL
Zn in 9GN6
Zn in 9GN7
Zn in 9GKU
Zn in 9GKW
Zn in 9GKX
Zn in 9GL0
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy