Chlorine in PDB 8a83: RSEGFP2 with A Chlorinated Chromophore in the Fluorescent on-State in A Crystal Dehydrated After Illumination

The structure of RSEGFP2 with A Chlorinated Chromophore in the Fluorescent on-State in A Crystal Dehydrated After Illumination, PDB code: 8a83 was solved by A.Fadini, J.Van Thor, J.Chang, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	38.79 / 1.81
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	51.484, 58.98, 64.484, 90, 90, 90
R / Rfree (%)	20 / 22.9

The binding sites of Chlorine atom in the RSEGFP2 with A Chlorinated Chromophore in the Fluorescent on-State in A Crystal Dehydrated After Illumination (pdb code 8a83). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total only one binding site of Chlorine was determined in the RSEGFP2 with A Chlorinated Chromophore in the Fluorescent on-State in A Crystal Dehydrated After Illumination, PDB code: 8a83:

Chlorine binding site 1 out of 1 in the RSEGFP2 with A Chlorinated Chromophore in the Fluorescent on-State in A Crystal Dehydrated After Illumination


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of RSEGFP2 with A Chlorinated Chromophore in the Fluorescent on-State in A Crystal Dehydrated After Illumination within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl68 b:35.1 occ:1.00 CL A:OHD68 0.0 35.1 1.0 CE2 A:OHD68 1.8 32.8 1.0 CD2 A:OHD68 2.8 30.8 1.0 CZ A:OHD68 2.9 34.5 1.0 OH A:OHD68 3.2 33.1 1.0 CZ A:PHE166 3.4 28.3 1.0 CE1 A:PHE166 3.5 27.4 1.0 OE1 A:GLN184 3.5 26.4 1.0 NH2 A:ARG97 3.7 26.9 1.0 CG2 A:OHD68 4.1 30.5 1.0 CD1 A:LEU70 4.1 26.0 1.0 CG1 A:VAL151 4.1 33.7 0.4 CG2 A:VAL151 4.2 33.4 0.6 CE1 A:OHD68 4.2 32.5 1.0 CE2 A:PHE166 4.2 29.1 1.0 CG1 A:VAL151 4.2 29.9 0.6 CG2 A:VAL151 4.2 28.9 0.4 CD1 A:PHE166 4.4 31.1 1.0 CB A:VAL151 4.5 31.1 0.6 CB A:VAL151 4.5 35.3 0.4 O2 A:OHD68 4.5 28.1 1.0 CD A:GLN184 4.6 30.0 1.0 CD1 A:OHD68 4.6 34.5 1.0 CZ A:ARG97 4.7 24.9 1.0 CD2 A:LEU70 4.8 24.5 1.0 NE2 A:GLN184 4.8 30.3 1.0 CD2 A:PHE166 4.9 29.1 1.0 NE A:ARG97 4.9 26.7 1.0

A.Fadini, C.D.M.Hutchison, D.Morozov, J.Chang, K.Maghlaoui, S.Perrett, F.Luo, J.C.X.Kho, M.G.Romei, R.M.L.Morgan, C.M.Orr, V.Cordon-Preciado, T.Fujiwara, N.Nuemket, T.Tosha, R.Tanaka, S.Owada, K.Tono, S.Iwata, S.G.Boxer, G.Groenhof, E.Nango, J.J.Van Thor. Serial Femtosecond Crystallography Reveals That Photoactivation in A Fluorescent Protein Proceeds Via the Hula Twist Mechanism. J.Am.Chem.Soc. 2023.
ISSN: ESSN 1520-5126
PubMed: 37418747
DOI: 10.1021/JACS.3C02313