Chlorine in PDB 8b06: Tryptophan Synthase - Cryo-Trapping By the Spitrobot Crystal Plunger After 25 Sec

All present enzymatic activity of Tryptophan Synthase - Cryo-Trapping By the Spitrobot Crystal Plunger After 25 Sec:
4.2.1.20;

The structure of Tryptophan Synthase - Cryo-Trapping By the Spitrobot Crystal Plunger After 25 Sec, PDB code: 8b06 was solved by P.Mehrabi, S.Sung, D.Von Stetten, A.Prester, C.E.Hatton, S.Kleine-Dopke, A.Berkes, G.Gore, J.P.Leimkohl, H.Schikora, M.Kollewe, H.Rohde, M.Wilmanns, F.Tellkamp, E.C.Schulz, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	43.54 / 2.49
Space group	C 1 2 1
Cell size a, b, c (Å), α, β, γ (°)	181.789, 59.048, 67.092, 90, 94.68, 90
R / Rfree (%)	22.8 / 26.6

The structure of Tryptophan Synthase - Cryo-Trapping By the Spitrobot Crystal Plunger After 25 Sec also contains other interesting chemical elements:

Caesium

(Cs)

1 atom

The binding sites of Chlorine atom in the Tryptophan Synthase - Cryo-Trapping By the Spitrobot Crystal Plunger After 25 Sec (pdb code 8b06). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 2 binding sites of Chlorine where determined in the Tryptophan Synthase - Cryo-Trapping By the Spitrobot Crystal Plunger After 25 Sec, PDB code: 8b06:
Jump to Chlorine binding site number: 1; 2;

Chlorine binding site 1 out of 2 in the Tryptophan Synthase - Cryo-Trapping By the Spitrobot Crystal Plunger After 25 Sec


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Tryptophan Synthase - Cryo-Trapping By the Spitrobot Crystal Plunger After 25 Sec within 5.0Å range: probe atom residue distance (Å) B Occ B:Cl504 b:43.0 occ:0.50 O B:HOH631 2.7 45.4 0.5 O B:PRO56 3.1 42.0 1.0 O B:GLY54 3.2 41.9 1.0 O B:HOH626 4.1 45.5 1.0 C B:GLY54 4.3 41.8 1.0 C B:PRO56 4.3 41.6 1.0 N B:PRO56 4.7 42.1 1.0 C B:ARG55 4.8 41.6 1.0 CD B:PRO56 4.9 42.6 1.0 O B:ARG55 4.9 41.9 1.0 CA B:GLY54 4.9 42.3 1.0

Chlorine binding site 2 out of 2 in the Tryptophan Synthase - Cryo-Trapping By the Spitrobot Crystal Plunger After 25 Sec


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Tryptophan Synthase - Cryo-Trapping By the Spitrobot Crystal Plunger After 25 Sec within 5.0Å range: probe atom residue distance (Å) B Occ B:Cl505 b:63.6 occ:1.00 O B:HOH635 4.0 51.8 1.0 CG B:GLN215 4.2 43.5 1.0 CB B:ALA214 4.4 45.3 1.0 CG B:GLU211 4.5 42.4 1.0 CD B:GLN215 4.6 43.2 1.0 CB B:PRO56 4.6 41.9 1.0 O B:GLU211 4.7 42.9 1.0 CA B:GLU211 4.7 42.7 1.0 NE2 B:GLN215 4.7 43.3 1.0 CB B:GLU211 4.7 42.0 1.0 CG B:PRO56 4.8 42.5 1.0

P.Mehrabi, S.Sung, D.Von Stetten, A.Prester, C.E.Hatton, S.Kleine-Dopke, A.Berkes, G.Gore, J.P.Leimkohl, H.Schikora, M.Kollewe, H.Rohde, M.Wilmanns, F.Tellkamp, E.C.Schulz. Millisecond Cryo-Trapping By the Spitrobot Crystal Plunger Simplifies Time-Resolved Crystallography Nat Commun 2023.
ISSN: ESSN 2041-1723
DOI: 10.1038/S41467-023-37834-W