Chlorine in PDB 8b6s: X-Ray Structure of the Haloalkane Dehalogenase HALOTAG7 Fusion to the Green Fluorescent Protein Gfp (CHEMOG1) Labeled with A Chloroalkane Tetramethylrhodamine Fluorophore Substrate

Enzymatic activity of X-Ray Structure of the Haloalkane Dehalogenase HALOTAG7 Fusion to the Green Fluorescent Protein Gfp (CHEMOG1) Labeled with A Chloroalkane Tetramethylrhodamine Fluorophore Substrate

All present enzymatic activity of X-Ray Structure of the Haloalkane Dehalogenase HALOTAG7 Fusion to the Green Fluorescent Protein Gfp (CHEMOG1) Labeled with A Chloroalkane Tetramethylrhodamine Fluorophore Substrate:
3.8.1.5;

Protein crystallography data

The structure of X-Ray Structure of the Haloalkane Dehalogenase HALOTAG7 Fusion to the Green Fluorescent Protein Gfp (CHEMOG1) Labeled with A Chloroalkane Tetramethylrhodamine Fluorophore Substrate, PDB code: 8b6s was solved by M.Tarnawski, L.Hellweg, J.Hiblot, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 46.18 / 1.80
Space group P 1
Cell size a, b, c (Å), α, β, γ (°) 46.19, 63.71, 89.42, 93.56, 91.02, 90.85
R / Rfree (%) 17.3 / 20.1

Chlorine Binding Sites:

The binding sites of Chlorine atom in the X-Ray Structure of the Haloalkane Dehalogenase HALOTAG7 Fusion to the Green Fluorescent Protein Gfp (CHEMOG1) Labeled with A Chloroalkane Tetramethylrhodamine Fluorophore Substrate (pdb code 8b6s). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 2 binding sites of Chlorine where determined in the X-Ray Structure of the Haloalkane Dehalogenase HALOTAG7 Fusion to the Green Fluorescent Protein Gfp (CHEMOG1) Labeled with A Chloroalkane Tetramethylrhodamine Fluorophore Substrate, PDB code: 8b6s:
Jump to Chlorine binding site number: 1; 2;

Chlorine binding site 1 out of 2 in 8b6s

Go back to Chlorine Binding Sites List in 8b6s
Chlorine binding site 1 out of 2 in the X-Ray Structure of the Haloalkane Dehalogenase HALOTAG7 Fusion to the Green Fluorescent Protein Gfp (CHEMOG1) Labeled with A Chloroalkane Tetramethylrhodamine Fluorophore Substrate


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of X-Ray Structure of the Haloalkane Dehalogenase HALOTAG7 Fusion to the Green Fluorescent Protein Gfp (CHEMOG1) Labeled with A Chloroalkane Tetramethylrhodamine Fluorophore Substrate within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Cl602

b:21.2
occ:1.00
 NE1 A:TRP341 3.2 18.3 1.0
ND2 A:ASN275 3.4 19.9 1.0
N A:PRO440 3.5 18.4 1.0
CA A:PRO440 3.7 18.1 1.0
C20 A:OEH601 3.7 19.4 1.0
C A:PHE439 3.8 18.0 1.0
CD1 A:TRP341 3.8 18.8 1.0
CD A:PRO440 3.8 18.5 1.0
CB A:PHE439 3.8 19.4 1.0
CZ A:PHE402 3.9 20.3 1.0
CB A:ASN275 3.9 18.8 1.0
C19 A:OEH601 3.9 19.2 1.0
CD1 A:LEU443 4.0 19.3 1.0
O A:PHE439 4.0 18.1 1.0
CB A:PRO440 4.1 19.2 1.0
CG A:ASN275 4.1 22.4 1.0
CG A:LEU443 4.2 18.4 1.0
CD2 A:LEU443 4.2 17.2 1.0
CE2 A:PHE402 4.3 20.3 1.0
C18 A:OEH601 4.4 18.2 1.0
CA A:PHE439 4.4 19.1 1.0
CE2 A:TRP341 4.5 18.7 1.0
CG A:PRO440 4.5 18.9 1.0
CE2 A:PHE383 4.7 20.1 1.0
OD1 A:ASP340 4.8 18.2 1.0
CE1 A:PHE402 4.8 21.2 1.0
CG A:PHE439 4.8 18.4 1.0
OD2 A:ASP340 5.0 19.7 1.0

Chlorine binding site 2 out of 2 in 8b6s

Go back to Chlorine Binding Sites List in 8b6s
Chlorine binding site 2 out of 2 in the X-Ray Structure of the Haloalkane Dehalogenase HALOTAG7 Fusion to the Green Fluorescent Protein Gfp (CHEMOG1) Labeled with A Chloroalkane Tetramethylrhodamine Fluorophore Substrate


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of X-Ray Structure of the Haloalkane Dehalogenase HALOTAG7 Fusion to the Green Fluorescent Protein Gfp (CHEMOG1) Labeled with A Chloroalkane Tetramethylrhodamine Fluorophore Substrate within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Cl602

b:20.1
occ:1.00
 ND2 B:ASN275 3.3 16.2 1.0
NE1 B:TRP341 3.3 19.1 1.0
N B:PRO440 3.5 16.5 1.0
CA B:PRO440 3.7 17.6 1.0
C20 B:OEH601 3.7 18.5 1.0
CD B:PRO440 3.8 15.9 1.0
C B:PHE439 3.8 18.1 1.0
CD1 B:TRP341 3.8 18.5 1.0
CZ B:PHE402 3.8 18.6 1.0
CB B:ASN275 3.9 17.8 1.0
CB B:PHE439 3.9 17.9 1.0
C19 B:OEH601 3.9 18.6 1.0
CD1 B:LEU443 4.0 20.0 1.0
CB B:PRO440 4.1 17.9 1.0
CG B:ASN275 4.1 19.3 1.0
O B:PHE439 4.1 18.6 1.0
CG B:LEU443 4.1 17.8 1.0
CD2 B:LEU443 4.2 16.5 1.0
C18 B:OEH601 4.3 17.9 1.0
CE2 B:PHE402 4.3 19.9 1.0
CA B:PHE439 4.5 17.6 1.0
CG B:PRO440 4.5 17.5 1.0
CE2 B:TRP341 4.6 16.8 1.0
CE2 B:PHE383 4.6 20.3 1.0
CE1 B:PHE402 4.8 20.6 1.0
OD1 B:ASP340 4.8 17.0 1.0
CG B:PHE439 4.9 16.9 1.0
OD2 B:ASP340 5.0 18.9 1.0

Reference:

L.Hellweg, A.Edenhofer, L.Barck, M.C.Huppertz, M.S.Frei, M.Tarnawski, A.Bergner, B.Koch, K.Johnsson, J.Hiblot. A General Method For the Development of Multicolor Biosensors with Large Dynamic Ranges. Nat.Chem.Biol. 2023.
ISSN: ESSN 1552-4469
PubMed: 37291200
DOI: 10.1038/S41589-023-01350-1
Page generated: Tue Jul 30 07:02:04 2024

Last articles

Zn in 9JPJ
Zn in 9JP7
Zn in 9JPK
Zn in 9JPL
Zn in 9GN6
Zn in 9GN7
Zn in 9GKU
Zn in 9GKW
Zn in 9GKX
Zn in 9GL0
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy