Chlorine in PDB 8b6t: X-Ray Structure of the Interface Optimized Haloalkane Dehalogenase HALOTAG7 Fusion to the Green Fluorescent Protein Gfp (CHEMOG5-Tmr) Labeled with A Chloroalkane Tetramethylrhodamine Fluorophore Substrate

Enzymatic activity of X-Ray Structure of the Interface Optimized Haloalkane Dehalogenase HALOTAG7 Fusion to the Green Fluorescent Protein Gfp (CHEMOG5-Tmr) Labeled with A Chloroalkane Tetramethylrhodamine Fluorophore Substrate

All present enzymatic activity of X-Ray Structure of the Interface Optimized Haloalkane Dehalogenase HALOTAG7 Fusion to the Green Fluorescent Protein Gfp (CHEMOG5-Tmr) Labeled with A Chloroalkane Tetramethylrhodamine Fluorophore Substrate:
3.8.1.5;

Protein crystallography data

The structure of X-Ray Structure of the Interface Optimized Haloalkane Dehalogenase HALOTAG7 Fusion to the Green Fluorescent Protein Gfp (CHEMOG5-Tmr) Labeled with A Chloroalkane Tetramethylrhodamine Fluorophore Substrate, PDB code: 8b6t was solved by M.Tarnawski, L.Hellweg, J.Hiblot, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 46.18 / 2.00
Space group P 1 21 1
Cell size a, b, c (Å), α, β, γ (°) 46.6, 64.04, 172.95, 90, 97.67, 90
R / Rfree (%) 22.1 / 24.5

Chlorine Binding Sites:

The binding sites of Chlorine atom in the X-Ray Structure of the Interface Optimized Haloalkane Dehalogenase HALOTAG7 Fusion to the Green Fluorescent Protein Gfp (CHEMOG5-Tmr) Labeled with A Chloroalkane Tetramethylrhodamine Fluorophore Substrate (pdb code 8b6t). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 2 binding sites of Chlorine where determined in the X-Ray Structure of the Interface Optimized Haloalkane Dehalogenase HALOTAG7 Fusion to the Green Fluorescent Protein Gfp (CHEMOG5-Tmr) Labeled with A Chloroalkane Tetramethylrhodamine Fluorophore Substrate, PDB code: 8b6t:
Jump to Chlorine binding site number: 1; 2;

Chlorine binding site 1 out of 2 in 8b6t

Go back to Chlorine Binding Sites List in 8b6t
Chlorine binding site 1 out of 2 in the X-Ray Structure of the Interface Optimized Haloalkane Dehalogenase HALOTAG7 Fusion to the Green Fluorescent Protein Gfp (CHEMOG5-Tmr) Labeled with A Chloroalkane Tetramethylrhodamine Fluorophore Substrate


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of X-Ray Structure of the Interface Optimized Haloalkane Dehalogenase HALOTAG7 Fusion to the Green Fluorescent Protein Gfp (CHEMOG5-Tmr) Labeled with A Chloroalkane Tetramethylrhodamine Fluorophore Substrate within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Cl602

b:14.6
occ:1.00
 ND2 A:ASN275 3.3 13.7 1.0
NE1 A:TRP341 3.5 13.2 1.0
N A:PRO440 3.6 15.1 1.0
C20 A:OEH601 3.8 13.5 1.0
CZ A:PHE402 3.8 16.1 1.0
CA A:PRO440 3.8 16.3 1.0
CB A:ASN275 3.8 14.2 1.0
C A:PHE439 3.9 14.7 1.0
CD1 A:TRP341 3.9 11.8 1.0
CB A:PHE439 3.9 12.1 1.0
CD A:PRO440 4.0 12.7 1.0
C19 A:OEH601 4.0 16.8 1.0
CD1 A:LEU443 4.1 11.9 1.0
CG A:ASN275 4.1 12.0 1.0
CB A:PRO440 4.1 18.0 1.0
O A:PHE439 4.2 15.2 1.0
C18 A:OEH601 4.3 12.8 1.0
CG A:LEU443 4.4 11.8 1.0
CE2 A:PHE402 4.5 17.7 1.0
CE1 A:PHE402 4.6 16.9 1.0
CA A:PHE439 4.6 14.3 1.0
CG A:PRO440 4.6 14.8 1.0
CD2 A:LEU443 4.6 13.1 1.0
CE2 A:PHE383 4.6 18.0 1.0
OD1 A:ASP340 4.7 17.1 1.0
CE2 A:TRP341 4.7 10.5 1.0
CG A:PHE439 4.9 11.8 1.0
OD2 A:ASP340 4.9 12.2 1.0

Chlorine binding site 2 out of 2 in 8b6t

Go back to Chlorine Binding Sites List in 8b6t
Chlorine binding site 2 out of 2 in the X-Ray Structure of the Interface Optimized Haloalkane Dehalogenase HALOTAG7 Fusion to the Green Fluorescent Protein Gfp (CHEMOG5-Tmr) Labeled with A Chloroalkane Tetramethylrhodamine Fluorophore Substrate


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of X-Ray Structure of the Interface Optimized Haloalkane Dehalogenase HALOTAG7 Fusion to the Green Fluorescent Protein Gfp (CHEMOG5-Tmr) Labeled with A Chloroalkane Tetramethylrhodamine Fluorophore Substrate within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Cl602

b:13.6
occ:1.00
 NE1 B:TRP341 3.1 17.8 1.0
N B:PRO440 3.4 13.8 1.0
ND2 B:ASN275 3.5 13.7 1.0
CA B:PRO440 3.5 14.3 1.0
C B:PHE439 3.6 14.2 1.0
CD1 B:TRP341 3.8 13.0 1.0
CD B:PRO440 3.8 15.2 1.0
C20 B:OEH601 3.8 13.6 1.0
O B:PHE439 3.8 16.4 1.0
CB B:PHE439 3.8 14.0 1.0
CD1 B:LEU443 3.8 12.0 1.0
CG B:LEU443 3.9 11.9 1.0
CB B:PRO440 4.0 12.3 1.0
C19 B:OEH601 4.0 13.0 1.0
CB B:ASN275 4.1 15.1 1.0
CD2 B:LEU443 4.1 10.4 1.0
CZ B:PHE402 4.2 14.6 1.0
CG B:ASN275 4.3 12.5 1.0
CE2 B:TRP341 4.3 15.3 1.0
CA B:PHE439 4.3 16.4 1.0
CG B:PRO440 4.5 14.7 1.0
C18 B:OEH601 4.6 13.7 1.0
CE2 B:PHE383 4.7 15.0 1.0
CE2 B:PHE402 4.7 16.2 1.0
CG B:PHE439 4.8 12.2 1.0
OD1 B:ASP340 4.8 15.7 1.0
CZ2 B:TRP341 4.8 15.9 1.0
C B:PRO440 4.9 16.3 1.0

Reference:

L.Hellweg, A.Edenhofer, L.Barck, M.C.Huppertz, M.S.Frei, M.Tarnawski, A.Bergner, B.Koch, K.Johnsson, J.Hiblot. A General Method For the Development of Multicolor Biosensors with Large Dynamic Ranges. Nat.Chem.Biol. 2023.
ISSN: ESSN 1552-4469
PubMed: 37291200
DOI: 10.1038/S41589-023-01350-1
Page generated: Tue Jul 30 07:02:03 2024

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