Chlorine in PDB 8fpc: Conformation 1 of the Ligand Binding Domain (LBDCONF1) of GLUA2 Flip Q Isoform of Ampa Receptor in Complex with Gain-of-Function Tarp Gamma- 2, with 10MM CACL2, 150MM Nacl, 1MM MGCL2, 330UM Ctz, and 100MM Glutamate (Open-Canamg)

The binding sites of Chlorine atom in the Conformation 1 of the Ligand Binding Domain (LBDCONF1) of GLUA2 Flip Q Isoform of Ampa Receptor in Complex with Gain-of-Function Tarp Gamma- 2, with 10MM CACL2, 150MM Nacl, 1MM MGCL2, 330UM Ctz, and 100MM Glutamate (Open-Canamg) (pdb code 8fpc). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 4 binding sites of Chlorine where determined in the Conformation 1 of the Ligand Binding Domain (LBDCONF1) of GLUA2 Flip Q Isoform of Ampa Receptor in Complex with Gain-of-Function Tarp Gamma- 2, with 10MM CACL2, 150MM Nacl, 1MM MGCL2, 330UM Ctz, and 100MM Glutamate (Open-Canamg), PDB code: 8fpc:
Jump to Chlorine binding site number: 1; 2; 3; 4;

Chlorine binding site 1 out of 4 in the Conformation 1 of the Ligand Binding Domain (LBDCONF1) of GLUA2 Flip Q Isoform of Ampa Receptor in Complex with Gain-of-Function Tarp Gamma- 2, with 10MM CACL2, 150MM Nacl, 1MM MGCL2, 330UM Ctz, and 100MM Glutamate (Open-Canamg)


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Conformation 1 of the Ligand Binding Domain (LBDCONF1) of GLUA2 Flip Q Isoform of Ampa Receptor in Complex with Gain-of-Function Tarp Gamma- 2, with 10MM CACL2, 150MM Nacl, 1MM MGCL2, 330UM Ctz, and 100MM Glutamate (Open-Canamg) within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl1302 b:77.3 occ:1.00 CL A:CYZ1302 0.0 77.3 1.0 C13 A:CYZ1302 1.8 36.4 1.0 C12 A:CYZ1302 2.7 26.3 1.0 C14 A:CYZ1302 2.8 31.4 1.0 O4 A:CYZ1302 3.0 43.0 1.0 OD1 A:ASP760 3.1 52.9 1.0 S2 A:CYZ1302 3.2 48.8 1.0 CG A:ASP760 3.2 51.1 1.0 N A:ASP760 3.2 30.3 1.0 CB A:LEU759 3.4 27.1 1.0 N3 A:CYZ1302 3.4 40.2 1.0 OD2 A:ASP760 3.5 49.2 1.0 C A:LEU759 3.6 36.1 1.0 CA A:ASP760 3.6 27.0 1.0 O A:HOH1401 3.7 28.0 1.0 CB A:ASP760 3.8 33.3 1.0 C11 A:CYZ1302 4.0 19.5 1.0 C10 A:CYZ1302 4.1 31.8 1.0 CA A:LEU759 4.1 26.1 1.0 O A:LEU759 4.2 44.1 1.0 C9 A:CYZ1302 4.6 32.0 1.0 CG A:LEU759 4.6 30.3 1.0 O A:PHE495 4.6 39.3 1.0 CD1 A:LEU759 4.6 22.9 1.0 O3 A:CYZ1302 4.8 38.0 1.0 N A:LEU759 4.8 25.4 1.0 CB D:SER729 4.9 41.1 1.0

Chlorine binding site 2 out of 4 in the Conformation 1 of the Ligand Binding Domain (LBDCONF1) of GLUA2 Flip Q Isoform of Ampa Receptor in Complex with Gain-of-Function Tarp Gamma- 2, with 10MM CACL2, 150MM Nacl, 1MM MGCL2, 330UM Ctz, and 100MM Glutamate (Open-Canamg)


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Conformation 1 of the Ligand Binding Domain (LBDCONF1) of GLUA2 Flip Q Isoform of Ampa Receptor in Complex with Gain-of-Function Tarp Gamma- 2, with 10MM CACL2, 150MM Nacl, 1MM MGCL2, 330UM Ctz, and 100MM Glutamate (Open-Canamg) within 5.0Å range: probe atom residue distance (Å) B Occ B:Cl1302 b:82.9 occ:1.00 CL B:CYZ1302 0.0 82.9 1.0 C13 B:CYZ1302 1.8 53.3 1.0 C12 B:CYZ1302 2.7 48.8 1.0 C14 B:CYZ1302 2.8 45.5 1.0 O4 B:CYZ1302 2.9 47.8 1.0 S2 B:CYZ1302 3.2 65.5 1.0 N B:ASP760 3.3 45.9 1.0 O B:HOH1402 3.4 39.1 1.0 CB B:LEU759 3.5 40.9 1.0 C B:LEU759 3.5 39.6 1.0 CA B:ASP760 3.5 51.6 1.0 N3 B:CYZ1302 3.5 52.4 1.0 O B:LEU759 3.9 46.9 1.0 CB B:ASP760 3.9 54.8 1.0 OG C:SER729 4.0 46.8 1.0 C11 B:CYZ1302 4.0 46.2 1.0 C10 B:CYZ1302 4.1 42.1 1.0 CA B:LEU759 4.1 41.7 1.0 C9 B:CYZ1302 4.6 44.6 1.0 O B:PHE495 4.7 42.1 1.0 CG B:LEU759 4.7 39.5 1.0 O3 B:CYZ1302 4.8 59.4 1.0 CD1 B:LEU759 4.8 40.3 1.0 C B:ASP760 4.9 58.0 1.0

Chlorine binding site 3 out of 4 in the Conformation 1 of the Ligand Binding Domain (LBDCONF1) of GLUA2 Flip Q Isoform of Ampa Receptor in Complex with Gain-of-Function Tarp Gamma- 2, with 10MM CACL2, 150MM Nacl, 1MM MGCL2, 330UM Ctz, and 100MM Glutamate (Open-Canamg)


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 3 of Conformation 1 of the Ligand Binding Domain (LBDCONF1) of GLUA2 Flip Q Isoform of Ampa Receptor in Complex with Gain-of-Function Tarp Gamma- 2, with 10MM CACL2, 150MM Nacl, 1MM MGCL2, 330UM Ctz, and 100MM Glutamate (Open-Canamg) within 5.0Å range: probe atom residue distance (Å) B Occ C:Cl1302 b:78.4 occ:1.00 CL C:CYZ1302 0.0 78.4 1.0 C13 C:CYZ1302 1.8 34.4 1.0 C12 C:CYZ1302 2.7 27.4 1.0 C14 C:CYZ1302 2.8 29.7 1.0 O4 C:CYZ1302 3.0 42.1 1.0 OD1 C:ASP760 3.1 51.6 1.0 S2 C:CYZ1302 3.2 51.8 1.0 N C:ASP760 3.2 35.4 1.0 CG C:ASP760 3.2 51.1 1.0 CB C:LEU759 3.4 31.4 1.0 N3 C:CYZ1302 3.4 42.4 1.0 OD2 C:ASP760 3.5 49.8 1.0 C C:LEU759 3.6 37.4 1.0 CA C:ASP760 3.6 29.7 1.0 O C:HOH1401 3.6 26.7 1.0 CB C:ASP760 3.8 36.7 1.0 C11 C:CYZ1302 4.0 22.5 1.0 C10 C:CYZ1302 4.1 30.4 1.0 CA C:LEU759 4.1 24.3 1.0 O C:LEU759 4.1 43.9 1.0 C9 C:CYZ1302 4.6 29.8 1.0 O C:PHE495 4.6 41.1 1.0 CG C:LEU759 4.7 33.7 1.0 CD1 C:LEU759 4.7 24.2 1.0 O3 C:CYZ1302 4.8 41.3 1.0 N C:LEU759 4.9 25.5 1.0 CB B:SER729 4.9 38.8 1.0

Chlorine binding site 4 out of 4 in the Conformation 1 of the Ligand Binding Domain (LBDCONF1) of GLUA2 Flip Q Isoform of Ampa Receptor in Complex with Gain-of-Function Tarp Gamma- 2, with 10MM CACL2, 150MM Nacl, 1MM MGCL2, 330UM Ctz, and 100MM Glutamate (Open-Canamg)


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 4 of Conformation 1 of the Ligand Binding Domain (LBDCONF1) of GLUA2 Flip Q Isoform of Ampa Receptor in Complex with Gain-of-Function Tarp Gamma- 2, with 10MM CACL2, 150MM Nacl, 1MM MGCL2, 330UM Ctz, and 100MM Glutamate (Open-Canamg) within 5.0Å range: probe atom residue distance (Å) B Occ D:Cl1302 b:85.7 occ:1.00 CL D:CYZ1302 0.0 85.7 1.0 C13 D:CYZ1302 1.8 54.1 1.0 C12 D:CYZ1302 2.7 47.5 1.0 C14 D:CYZ1302 2.8 47.3 1.0 O4 D:CYZ1302 2.9 46.9 1.0 S2 D:CYZ1302 3.2 66.6 1.0 N D:ASP760 3.3 42.5 1.0 O D:HOH1402 3.4 39.2 1.0 CA D:ASP760 3.5 51.0 1.0 C D:LEU759 3.5 40.4 1.0 CB D:LEU759 3.5 40.4 1.0 N3 D:CYZ1302 3.6 52.9 1.0 O D:LEU759 3.9 47.2 1.0 CB D:ASP760 3.9 52.2 1.0 OG A:SER729 4.0 48.4 1.0 C11 D:CYZ1302 4.0 44.5 1.0 C10 D:CYZ1302 4.1 42.7 1.0 CA D:LEU759 4.2 44.4 1.0 C9 D:CYZ1302 4.6 44.0 1.0 O D:PHE495 4.6 44.0 1.0 O3 D:CYZ1302 4.8 58.7 1.0 CG D:LEU759 4.8 40.2 1.0 C D:ASP760 4.9 58.7 1.0 CD1 D:LEU759 4.9 40.3 1.0

T.Nakagawa, X.Wang, F.J.Miguez-Cabello, D.Bowie. The Open Gate of the Ampa Receptor Forms A CA2+ Binding Site Critical in Regulating Ion Transport Nat.Struct.Mol.Biol. 2024.
ISSN: ESSN 1545-9985
DOI: 10.1038/S41594-024-01228-3