Chlorine in PDB 8fph: Conformation 2 of the Ligand Binding Domain (LBDCONF2) of GLUA2 Flip Q Isoform of Ampa Receptor in Complex with Gain-of-Function Tarp Gamma- 2, with 10MM CACL2, 150MM Nacl, 1MM MGCL2, 330UM Ctz, and 100MM Glutamate (Open-Canamg)

The binding sites of Chlorine atom in the Conformation 2 of the Ligand Binding Domain (LBDCONF2) of GLUA2 Flip Q Isoform of Ampa Receptor in Complex with Gain-of-Function Tarp Gamma- 2, with 10MM CACL2, 150MM Nacl, 1MM MGCL2, 330UM Ctz, and 100MM Glutamate (Open-Canamg) (pdb code 8fph). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 4 binding sites of Chlorine where determined in the Conformation 2 of the Ligand Binding Domain (LBDCONF2) of GLUA2 Flip Q Isoform of Ampa Receptor in Complex with Gain-of-Function Tarp Gamma- 2, with 10MM CACL2, 150MM Nacl, 1MM MGCL2, 330UM Ctz, and 100MM Glutamate (Open-Canamg), PDB code: 8fph:
Jump to Chlorine binding site number: 1; 2; 3; 4;

Chlorine binding site 1 out of 4 in the Conformation 2 of the Ligand Binding Domain (LBDCONF2) of GLUA2 Flip Q Isoform of Ampa Receptor in Complex with Gain-of-Function Tarp Gamma- 2, with 10MM CACL2, 150MM Nacl, 1MM MGCL2, 330UM Ctz, and 100MM Glutamate (Open-Canamg)


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Conformation 2 of the Ligand Binding Domain (LBDCONF2) of GLUA2 Flip Q Isoform of Ampa Receptor in Complex with Gain-of-Function Tarp Gamma- 2, with 10MM CACL2, 150MM Nacl, 1MM MGCL2, 330UM Ctz, and 100MM Glutamate (Open-Canamg) within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl1302 b:57.7 occ:1.00 CL A:CYZ1302 0.0 57.7 1.0 C13 A:CYZ1302 1.8 29.0 1.0 C12 A:CYZ1302 2.7 24.5 1.0 C14 A:CYZ1302 2.8 22.6 1.0 O4 A:CYZ1302 2.9 35.0 1.0 N A:ASP760 3.2 23.5 1.0 S2 A:CYZ1302 3.2 41.4 1.0 OD2 A:ASP760 3.3 38.9 1.0 CG A:ASP760 3.3 41.7 1.0 CB A:LEU759 3.4 28.5 1.0 N3 A:CYZ1302 3.5 33.4 1.0 CA A:ASP760 3.5 27.9 1.0 C A:LEU759 3.5 30.5 1.0 OD1 A:ASP760 3.6 44.0 1.0 O A:HOH1401 3.7 13.7 1.0 CB A:ASP760 3.8 35.0 1.0 C11 A:CYZ1302 4.0 29.7 1.0 O A:LEU759 4.1 41.8 1.0 C10 A:CYZ1302 4.1 27.6 1.0 CA A:LEU759 4.1 21.5 1.0 C9 A:CYZ1302 4.6 28.8 1.0 CG A:LEU759 4.6 25.5 1.0 O A:PHE495 4.6 35.3 1.0 CD1 A:LEU759 4.6 21.6 1.0 O3 A:CYZ1302 4.8 28.6 1.0 N A:LEU759 4.9 22.4 1.0

Chlorine binding site 2 out of 4 in the Conformation 2 of the Ligand Binding Domain (LBDCONF2) of GLUA2 Flip Q Isoform of Ampa Receptor in Complex with Gain-of-Function Tarp Gamma- 2, with 10MM CACL2, 150MM Nacl, 1MM MGCL2, 330UM Ctz, and 100MM Glutamate (Open-Canamg)


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Conformation 2 of the Ligand Binding Domain (LBDCONF2) of GLUA2 Flip Q Isoform of Ampa Receptor in Complex with Gain-of-Function Tarp Gamma- 2, with 10MM CACL2, 150MM Nacl, 1MM MGCL2, 330UM Ctz, and 100MM Glutamate (Open-Canamg) within 5.0Å range: probe atom residue distance (Å) B Occ B:Cl1302 b:63.0 occ:1.00 CL B:CYZ1302 0.0 63.0 1.0 C13 B:CYZ1302 1.8 46.6 1.0 C12 B:CYZ1302 2.7 46.6 1.0 C14 B:CYZ1302 2.8 46.8 1.0 O4 B:CYZ1302 3.0 49.4 1.0 S2 B:CYZ1302 3.2 65.8 1.0 N B:ASP760 3.3 60.5 1.0 N3 B:CYZ1302 3.4 50.1 1.0 CB B:LEU759 3.5 48.9 1.0 CA B:ASP760 3.6 60.5 1.0 C B:LEU759 3.7 54.7 1.0 O B:HOH1402 3.7 52.2 1.0 CB B:ASP760 3.9 63.7 1.0 C11 B:CYZ1302 4.0 45.1 1.0 C10 B:CYZ1302 4.1 44.8 1.0 O B:LEU759 4.2 55.2 1.0 CA B:LEU759 4.2 51.4 1.0 C9 B:CYZ1302 4.6 45.7 1.0 CG B:LEU759 4.7 46.1 1.0 CD1 B:LEU759 4.7 45.0 1.0 O3 B:CYZ1302 4.8 51.5 1.0 CG B:ASP760 4.9 69.2 1.0 OD1 B:ASP760 4.9 70.1 1.0 O B:PHE495 4.9 42.0 1.0 OG C:SER729 5.0 50.1 1.0 N B:LEU759 5.0 55.5 1.0

Chlorine binding site 3 out of 4 in the Conformation 2 of the Ligand Binding Domain (LBDCONF2) of GLUA2 Flip Q Isoform of Ampa Receptor in Complex with Gain-of-Function Tarp Gamma- 2, with 10MM CACL2, 150MM Nacl, 1MM MGCL2, 330UM Ctz, and 100MM Glutamate (Open-Canamg)


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 3 of Conformation 2 of the Ligand Binding Domain (LBDCONF2) of GLUA2 Flip Q Isoform of Ampa Receptor in Complex with Gain-of-Function Tarp Gamma- 2, with 10MM CACL2, 150MM Nacl, 1MM MGCL2, 330UM Ctz, and 100MM Glutamate (Open-Canamg) within 5.0Å range: probe atom residue distance (Å) B Occ C:Cl1302 b:56.3 occ:1.00 CL C:CYZ1302 0.0 56.3 1.0 C13 C:CYZ1302 1.8 28.5 1.0 C12 C:CYZ1302 2.7 27.9 1.0 C14 C:CYZ1302 2.8 22.4 1.0 O4 C:CYZ1302 2.9 36.0 1.0 OD2 C:ASP760 3.1 48.3 1.0 S2 C:CYZ1302 3.2 43.8 1.0 N C:ASP760 3.2 27.4 1.0 CG C:ASP760 3.3 48.4 1.0 CB C:LEU759 3.4 33.0 1.0 C C:LEU759 3.5 34.7 1.0 N3 C:CYZ1302 3.5 36.3 1.0 O C:HOH1401 3.6 13.9 1.0 CA C:ASP760 3.6 33.7 1.0 OD1 C:ASP760 3.7 45.0 1.0 CB C:ASP760 3.9 40.8 1.0 O C:LEU759 4.0 45.3 1.0 C11 C:CYZ1302 4.0 33.3 1.0 CA C:LEU759 4.1 25.7 1.0 C10 C:CYZ1302 4.1 28.2 1.0 C9 C:CYZ1302 4.6 32.4 1.0 CG C:LEU759 4.6 28.8 1.0 O C:PHE495 4.6 39.2 1.0 CD1 C:LEU759 4.6 26.8 1.0 O3 C:CYZ1302 4.7 31.0 1.0 N C:LEU759 4.9 27.8 1.0 CB B:SER729 5.0 41.6 1.0

Chlorine binding site 4 out of 4 in the Conformation 2 of the Ligand Binding Domain (LBDCONF2) of GLUA2 Flip Q Isoform of Ampa Receptor in Complex with Gain-of-Function Tarp Gamma- 2, with 10MM CACL2, 150MM Nacl, 1MM MGCL2, 330UM Ctz, and 100MM Glutamate (Open-Canamg)


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 4 of Conformation 2 of the Ligand Binding Domain (LBDCONF2) of GLUA2 Flip Q Isoform of Ampa Receptor in Complex with Gain-of-Function Tarp Gamma- 2, with 10MM CACL2, 150MM Nacl, 1MM MGCL2, 330UM Ctz, and 100MM Glutamate (Open-Canamg) within 5.0Å range: probe atom residue distance (Å) B Occ D:Cl1302 b:59.9 occ:1.00 CL D:CYZ1302 0.0 59.9 1.0 C13 D:CYZ1302 1.8 44.5 1.0 C12 D:CYZ1302 2.7 45.5 1.0 C14 D:CYZ1302 2.8 44.2 1.0 O4 D:CYZ1302 3.0 46.0 1.0 N D:ASP760 3.2 58.7 1.0 S2 D:CYZ1302 3.2 60.5 1.0 N3 D:CYZ1302 3.5 48.5 1.0 CA D:ASP760 3.5 58.1 1.0 CB D:ASP760 3.5 62.8 1.0 CB D:LEU759 3.5 50.9 1.0 C D:LEU759 3.7 54.1 1.0 O D:HOH1402 3.7 48.1 1.0 C11 D:CYZ1302 4.0 43.1 1.0 C10 D:CYZ1302 4.1 40.8 1.0 CA D:LEU759 4.2 51.3 1.0 O D:LEU759 4.3 56.4 1.0 CG D:ASP760 4.5 64.9 1.0 C9 D:CYZ1302 4.6 40.8 1.0 O3 D:CYZ1302 4.8 43.3 1.0 CG D:LEU759 4.8 46.0 1.0 CD1 D:LEU759 4.8 43.6 1.0 O D:PHE495 4.9 41.0 1.0 N D:LEU759 4.9 56.1 1.0 OD1 D:ASP760 4.9 62.7 1.0 OG A:SER729 5.0 50.1 1.0

T.Nakagawa, X.Wang, F.J.Miguez-Cabello, D.Bowie. The Open Gate of the Ampa Receptor Forms A CA2+ Binding Site Critical in Regulating Ion Transport Nat.Struct.Mol.Biol. 2024.
ISSN: ESSN 1545-9985
DOI: 10.1038/S41594-024-01228-3