Chlorine in PDB 8fqb: GLUA2 Flip Q Isoform of Ampa Receptor in Complex with Gain-of-Function Tarp GAMMA2, with 10MM CACL2, 140MM Nmdg, 330UM Ctz, and 100MM L- Glutamate (Open-CA10)

The structure of GLUA2 Flip Q Isoform of Ampa Receptor in Complex with Gain-of-Function Tarp GAMMA2, with 10MM CACL2, 140MM Nmdg, 330UM Ctz, and 100MM L- Glutamate (Open-CA10) also contains other interesting chemical elements:

Calcium

(Ca)

1 atom

The binding sites of Chlorine atom in the GLUA2 Flip Q Isoform of Ampa Receptor in Complex with Gain-of-Function Tarp GAMMA2, with 10MM CACL2, 140MM Nmdg, 330UM Ctz, and 100MM L- Glutamate (Open-CA10) (pdb code 8fqb). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 2 binding sites of Chlorine where determined in the GLUA2 Flip Q Isoform of Ampa Receptor in Complex with Gain-of-Function Tarp GAMMA2, with 10MM CACL2, 140MM Nmdg, 330UM Ctz, and 100MM L- Glutamate (Open-CA10), PDB code: 8fqb:
Jump to Chlorine binding site number: 1; 2;

Chlorine binding site 1 out of 2 in the GLUA2 Flip Q Isoform of Ampa Receptor in Complex with Gain-of-Function Tarp GAMMA2, with 10MM CACL2, 140MM Nmdg, 330UM Ctz, and 100MM L- Glutamate (Open-CA10)


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of GLUA2 Flip Q Isoform of Ampa Receptor in Complex with Gain-of-Function Tarp GAMMA2, with 10MM CACL2, 140MM Nmdg, 330UM Ctz, and 100MM L- Glutamate (Open-CA10) within 5.0Å range: probe atom residue distance (Å) B Occ F:Cl401 b:79.4 occ:1.00 N F:TRP64 2.9 58.4 1.0 N F:LEU63 2.9 41.0 1.0 CB F:ASP31 3.4 52.9 1.0 N F:ARG65 3.4 56.9 1.0 CA F:GLY62 3.5 34.0 1.0 C F:GLY62 3.6 36.1 1.0 CA F:ASP31 3.6 48.8 1.0 CD1 F:TRP64 3.6 60.4 1.0 CA F:TRP64 3.7 61.7 1.0 OD2 F:ASP31 3.8 55.7 1.0 O F:THR30 3.8 47.5 1.0 NH1 F:ARG65 3.8 58.9 1.0 C F:LEU63 3.8 41.5 1.0 CA F:LEU63 3.8 41.9 1.0 CB F:TRP64 3.8 60.7 1.0 O F:ARG65 3.9 57.9 1.0 N F:GLY62 3.9 34.7 1.0 CG F:TRP64 4.0 61.2 1.0 C F:TRP64 4.1 61.9 1.0 CG F:ASP31 4.1 55.9 1.0 CG F:ARG65 4.1 56.1 1.0 N F:ASP31 4.2 50.1 1.0 C F:THR30 4.2 46.3 1.0 CB F:LEU63 4.4 44.0 1.0 CZ F:ARG65 4.4 59.2 1.0 O F:GLY29 4.4 39.4 1.0 CA F:ARG65 4.5 56.9 1.0 C F:ARG65 4.6 56.7 1.0 NE1 F:TRP64 4.7 59.4 1.0 O F:GLY62 4.7 39.9 1.0 O F:VAL28 4.8 39.8 1.0 C F:ASP31 4.9 49.8 1.0 NH2 F:ARG65 4.9 59.3 1.0 CB F:ARG65 4.9 58.1 1.0 O F:LEU63 5.0 43.7 1.0

Chlorine binding site 2 out of 2 in the GLUA2 Flip Q Isoform of Ampa Receptor in Complex with Gain-of-Function Tarp GAMMA2, with 10MM CACL2, 140MM Nmdg, 330UM Ctz, and 100MM L- Glutamate (Open-CA10)


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of GLUA2 Flip Q Isoform of Ampa Receptor in Complex with Gain-of-Function Tarp GAMMA2, with 10MM CACL2, 140MM Nmdg, 330UM Ctz, and 100MM L- Glutamate (Open-CA10) within 5.0Å range: probe atom residue distance (Å) B Occ H:Cl401 b:80.1 occ:1.00 N H:TRP64 2.9 60.6 1.0 N H:LEU63 2.9 42.9 1.0 CB H:ASP31 3.4 52.4 1.0 N H:ARG65 3.4 57.4 1.0 CA H:GLY62 3.5 33.1 1.0 C H:GLY62 3.6 35.3 1.0 CD1 H:TRP64 3.6 62.2 1.0 CA H:ASP31 3.6 48.2 1.0 CA H:TRP64 3.7 63.9 1.0 C H:LEU63 3.8 43.5 1.0 CA H:LEU63 3.8 43.6 1.0 OD2 H:ASP31 3.8 55.2 1.0 CB H:TRP64 3.8 63.3 1.0 O H:THR30 3.8 48.4 1.0 NH1 H:ARG65 3.8 59.2 1.0 O H:ARG65 3.9 57.6 1.0 N H:GLY62 3.9 33.5 1.0 CG H:TRP64 4.0 63.5 1.0 C H:TRP64 4.1 63.7 1.0 CG H:ASP31 4.1 55.4 1.0 N H:ASP31 4.2 50.4 1.0 CG H:ARG65 4.2 56.2 1.0 C H:THR30 4.2 47.4 1.0 CB H:LEU63 4.4 45.8 1.0 O H:GLY29 4.4 39.5 1.0 CZ H:ARG65 4.4 59.4 1.0 CA H:ARG65 4.5 57.3 1.0 NE1 H:TRP64 4.6 62.0 1.0 C H:ARG65 4.6 57.0 1.0 O H:GLY62 4.7 38.9 1.0 O H:VAL28 4.8 41.0 1.0 C H:ASP31 4.9 49.4 1.0 NH2 H:ARG65 4.9 59.4 1.0 CB H:ARG65 4.9 57.7 1.0 O H:LEU63 5.0 45.7 1.0

T.Nakagawa, X.Wang, F.J.Miguez-Cabello, D.Bowie. The Open Gate of the Ampa Receptor Forms A CA2+ Binding Site Critical in Regulating Ion Transport Nat.Struct.Mol.Biol. 2024.
ISSN: ESSN 1545-9985
DOI: 10.1038/S41594-024-01228-3