Chlorine in PDB 8fqd: Lbd Conformation 1 (LBDCONF1) of GLUA2 Flip Q Isoform of Ampa Receptor in Complex with Gain-of-Function Tarp GAMMA2, with 10MM CACL2, 140MM Nmdg, 330UM Ctz, and 100MM L-Glutamate (Open-CA10)

The binding sites of Chlorine atom in the Lbd Conformation 1 (LBDCONF1) of GLUA2 Flip Q Isoform of Ampa Receptor in Complex with Gain-of-Function Tarp GAMMA2, with 10MM CACL2, 140MM Nmdg, 330UM Ctz, and 100MM L-Glutamate (Open-CA10) (pdb code 8fqd). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 4 binding sites of Chlorine where determined in the Lbd Conformation 1 (LBDCONF1) of GLUA2 Flip Q Isoform of Ampa Receptor in Complex with Gain-of-Function Tarp GAMMA2, with 10MM CACL2, 140MM Nmdg, 330UM Ctz, and 100MM L-Glutamate (Open-CA10), PDB code: 8fqd:
Jump to Chlorine binding site number: 1; 2; 3; 4;

Chlorine binding site 1 out of 4 in the Lbd Conformation 1 (LBDCONF1) of GLUA2 Flip Q Isoform of Ampa Receptor in Complex with Gain-of-Function Tarp GAMMA2, with 10MM CACL2, 140MM Nmdg, 330UM Ctz, and 100MM L-Glutamate (Open-CA10)


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Lbd Conformation 1 (LBDCONF1) of GLUA2 Flip Q Isoform of Ampa Receptor in Complex with Gain-of-Function Tarp GAMMA2, with 10MM CACL2, 140MM Nmdg, 330UM Ctz, and 100MM L-Glutamate (Open-CA10) within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl1302 b:68.8 occ:1.00 CL A:CYZ1302 0.0 68.8 1.0 C13 A:CYZ1302 1.8 31.8 1.0 C12 A:CYZ1302 2.7 29.4 1.0 C14 A:CYZ1302 2.8 30.0 1.0 O4 A:CYZ1302 2.8 45.5 1.0 OD1 A:ASP760 2.9 38.5 1.0 S2 A:CYZ1302 3.1 43.4 1.0 O A:HOH1401 3.3 0.0 1.0 CB A:LEU759 3.4 24.1 1.0 N3 A:CYZ1302 3.5 34.2 1.0 N A:ASP760 3.8 23.0 1.0 C A:LEU759 3.8 25.4 1.0 C11 A:CYZ1302 4.0 26.9 1.0 C10 A:CYZ1302 4.1 27.5 1.0 CG A:ASP760 4.1 41.7 1.0 O A:PHE495 4.2 21.0 1.0 O A:LEU759 4.2 41.0 1.0 CA A:ASP760 4.2 28.8 1.0 CA A:LEU759 4.3 17.6 1.0 CD1 A:LEU759 4.5 23.1 1.0 CG A:LEU759 4.6 29.1 1.0 C9 A:CYZ1302 4.6 25.9 1.0 O3 A:CYZ1302 4.7 33.1 1.0 CB A:ASP760 4.8 31.8 1.0 CB D:SER729 5.0 37.8 1.0

Chlorine binding site 2 out of 4 in the Lbd Conformation 1 (LBDCONF1) of GLUA2 Flip Q Isoform of Ampa Receptor in Complex with Gain-of-Function Tarp GAMMA2, with 10MM CACL2, 140MM Nmdg, 330UM Ctz, and 100MM L-Glutamate (Open-CA10)


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Lbd Conformation 1 (LBDCONF1) of GLUA2 Flip Q Isoform of Ampa Receptor in Complex with Gain-of-Function Tarp GAMMA2, with 10MM CACL2, 140MM Nmdg, 330UM Ctz, and 100MM L-Glutamate (Open-CA10) within 5.0Å range: probe atom residue distance (Å) B Occ B:Cl1302 b:81.1 occ:1.00 CL B:CYZ1302 0.0 81.1 1.0 C13 B:CYZ1302 1.8 56.1 1.0 C12 B:CYZ1302 2.8 50.0 1.0 C14 B:CYZ1302 2.8 47.7 1.0 O4 B:CYZ1302 3.0 50.5 1.0 S2 B:CYZ1302 3.2 55.5 1.0 N B:ASP760 3.3 55.9 1.0 N3 B:CYZ1302 3.5 43.8 1.0 CB B:LEU759 3.6 44.4 1.0 CA B:ASP760 3.6 58.7 1.0 C B:LEU759 3.7 48.6 1.0 CB B:ASP760 3.8 59.1 1.0 C11 B:CYZ1302 4.0 47.3 1.0 C10 B:CYZ1302 4.1 42.3 1.0 O B:LEU759 4.2 51.3 1.0 CA B:LEU759 4.2 47.9 1.0 OG C:SER729 4.3 37.2 1.0 CG B:ASP760 4.4 65.6 1.0 OD1 B:ASP760 4.5 66.0 1.0 C9 B:CYZ1302 4.6 43.9 1.0 O B:PHE495 4.7 43.6 1.0 CD1 B:LEU759 4.8 45.6 1.0 CG B:LEU759 4.8 44.8 1.0 O3 B:CYZ1302 4.8 51.4 1.0

Chlorine binding site 3 out of 4 in the Lbd Conformation 1 (LBDCONF1) of GLUA2 Flip Q Isoform of Ampa Receptor in Complex with Gain-of-Function Tarp GAMMA2, with 10MM CACL2, 140MM Nmdg, 330UM Ctz, and 100MM L-Glutamate (Open-CA10)


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 3 of Lbd Conformation 1 (LBDCONF1) of GLUA2 Flip Q Isoform of Ampa Receptor in Complex with Gain-of-Function Tarp GAMMA2, with 10MM CACL2, 140MM Nmdg, 330UM Ctz, and 100MM L-Glutamate (Open-CA10) within 5.0Å range: probe atom residue distance (Å) B Occ C:Cl1302 b:70.3 occ:1.00 CL C:CYZ1302 0.0 70.3 1.0 C13 C:CYZ1302 1.8 31.8 1.0 C12 C:CYZ1302 2.7 25.1 1.0 C14 C:CYZ1302 2.8 29.7 1.0 O4 C:CYZ1302 2.8 45.9 1.0 OD1 C:ASP760 3.0 46.7 1.0 S2 C:CYZ1302 3.2 36.8 1.0 CG C:ASP760 3.4 45.3 1.0 N C:ASP760 3.5 25.4 1.0 CB C:LEU759 3.5 28.2 1.0 N3 C:CYZ1302 3.6 33.5 1.0 O C:HOH1401 3.6 1.3 1.0 OD2 C:ASP760 3.7 45.2 1.0 C C:LEU759 3.8 27.6 1.0 CA C:ASP760 3.9 29.7 1.0 C11 C:CYZ1302 4.0 24.4 1.0 C10 C:CYZ1302 4.1 25.4 1.0 O C:LEU759 4.2 41.5 1.0 CB C:ASP760 4.2 34.8 1.0 CA C:LEU759 4.3 22.2 1.0 O C:PHE495 4.4 23.1 1.0 C9 C:CYZ1302 4.6 26.8 1.0 CD1 C:LEU759 4.6 26.6 1.0 CG C:LEU759 4.7 30.8 1.0 O3 C:CYZ1302 4.7 27.5 1.0 CB B:SER729 4.9 37.6 1.0

Chlorine binding site 4 out of 4 in the Lbd Conformation 1 (LBDCONF1) of GLUA2 Flip Q Isoform of Ampa Receptor in Complex with Gain-of-Function Tarp GAMMA2, with 10MM CACL2, 140MM Nmdg, 330UM Ctz, and 100MM L-Glutamate (Open-CA10)


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 4 of Lbd Conformation 1 (LBDCONF1) of GLUA2 Flip Q Isoform of Ampa Receptor in Complex with Gain-of-Function Tarp GAMMA2, with 10MM CACL2, 140MM Nmdg, 330UM Ctz, and 100MM L-Glutamate (Open-CA10) within 5.0Å range: probe atom residue distance (Å) B Occ D:Cl1302 b:84.2 occ:1.00 CL D:CYZ1302 0.0 84.2 1.0 C13 D:CYZ1302 1.8 57.9 1.0 C12 D:CYZ1302 2.7 52.2 1.0 C14 D:CYZ1302 2.8 49.2 1.0 O4 D:CYZ1302 3.0 53.9 1.0 S2 D:CYZ1302 3.2 57.8 1.0 N D:ASP760 3.3 51.9 1.0 CB D:ASP760 3.4 57.5 1.0 CA D:ASP760 3.5 56.3 1.0 N3 D:CYZ1302 3.5 48.4 1.0 CB D:LEU759 3.7 43.8 1.0 C D:LEU759 3.8 47.3 1.0 C11 D:CYZ1302 4.0 49.3 1.0 OG A:SER729 4.1 41.6 1.0 C10 D:CYZ1302 4.1 45.5 1.0 CA D:LEU759 4.4 47.5 1.0 O D:LEU759 4.4 49.3 1.0 C9 D:CYZ1302 4.6 46.0 1.0 O D:PHE495 4.7 41.9 1.0 CG D:ASP760 4.7 60.9 1.0 CD1 D:LEU759 4.8 44.8 1.0 O3 D:CYZ1302 4.8 52.5 1.0 CG D:LEU759 4.9 43.9 1.0 C D:ASP760 5.0 58.8 1.0

T.Nakagawa, X.Wang, F.J.Miguez-Cabello, D.Bowie. The Open Gate of the Ampa Receptor Forms A CA2+ Binding Site Critical in Regulating Ion Transport Nat.Struct.Mol.Biol. 2023.
ISSN: ESSN 1545-9985
DOI: 10.1038/S41594-024-01228-3