Chlorine in PDB 8fqe: Lbd Conformation 2 (LBDCONF2) of GLUA2 Flip Q Isoform of Ampa Receptor in Complex with Gain-of-Function Tarp GAMMA2, with 10MM CACL2, 140MM Nmdg, 330UM Ctz, and 100MM L-Glutamate (Open-CA10)

The binding sites of Chlorine atom in the Lbd Conformation 2 (LBDCONF2) of GLUA2 Flip Q Isoform of Ampa Receptor in Complex with Gain-of-Function Tarp GAMMA2, with 10MM CACL2, 140MM Nmdg, 330UM Ctz, and 100MM L-Glutamate (Open-CA10) (pdb code 8fqe). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 4 binding sites of Chlorine where determined in the Lbd Conformation 2 (LBDCONF2) of GLUA2 Flip Q Isoform of Ampa Receptor in Complex with Gain-of-Function Tarp GAMMA2, with 10MM CACL2, 140MM Nmdg, 330UM Ctz, and 100MM L-Glutamate (Open-CA10), PDB code: 8fqe:
Jump to Chlorine binding site number: 1; 2; 3; 4;

Chlorine binding site 1 out of 4 in the Lbd Conformation 2 (LBDCONF2) of GLUA2 Flip Q Isoform of Ampa Receptor in Complex with Gain-of-Function Tarp GAMMA2, with 10MM CACL2, 140MM Nmdg, 330UM Ctz, and 100MM L-Glutamate (Open-CA10)


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Lbd Conformation 2 (LBDCONF2) of GLUA2 Flip Q Isoform of Ampa Receptor in Complex with Gain-of-Function Tarp GAMMA2, with 10MM CACL2, 140MM Nmdg, 330UM Ctz, and 100MM L-Glutamate (Open-CA10) within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl1302 b:70.7 occ:1.00 CL A:CYZ1302 0.0 70.7 1.0 C13 A:CYZ1302 1.8 44.6 1.0 C12 A:CYZ1302 2.7 45.8 1.0 C14 A:CYZ1302 2.8 41.2 1.0 O4 A:CYZ1302 2.8 58.9 1.0 S2 A:CYZ1302 3.1 62.0 1.0 N A:ASP760 3.3 52.6 1.0 N3 A:CYZ1302 3.4 55.0 1.0 CA A:ASP760 3.5 49.9 1.0 OD1 A:ASP760 3.5 63.5 1.0 CG A:ASP760 3.6 62.1 1.0 CB A:ASP760 3.7 55.3 1.0 C A:LEU759 3.8 45.6 1.0 CB A:LEU759 4.0 51.9 1.0 C11 A:CYZ1302 4.0 44.3 1.0 O A:HOH1401 4.0 0.0 1.0 C10 A:CYZ1302 4.1 42.5 1.0 O A:LEU759 4.2 48.3 1.0 OD2 A:ASP760 4.3 54.7 1.0 CA A:LEU759 4.5 38.9 1.0 C9 A:CYZ1302 4.5 44.0 1.0 O3 A:CYZ1302 4.7 45.3 1.0 C A:ASP760 4.9 52.9 1.0 O A:PHE495 5.0 36.4 1.0

Chlorine binding site 2 out of 4 in the Lbd Conformation 2 (LBDCONF2) of GLUA2 Flip Q Isoform of Ampa Receptor in Complex with Gain-of-Function Tarp GAMMA2, with 10MM CACL2, 140MM Nmdg, 330UM Ctz, and 100MM L-Glutamate (Open-CA10)


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Lbd Conformation 2 (LBDCONF2) of GLUA2 Flip Q Isoform of Ampa Receptor in Complex with Gain-of-Function Tarp GAMMA2, with 10MM CACL2, 140MM Nmdg, 330UM Ctz, and 100MM L-Glutamate (Open-CA10) within 5.0Å range: probe atom residue distance (Å) B Occ B:Cl1302 b:94.5 occ:1.00 CL B:CYZ1302 0.0 94.5 1.0 C13 B:CYZ1302 1.8 67.6 1.0 C14 B:CYZ1302 2.7 61.5 1.0 C12 B:CYZ1302 2.8 64.4 1.0 O4 B:CYZ1302 3.2 66.0 1.0 S2 B:CYZ1302 3.3 75.3 1.0 N3 B:CYZ1302 3.4 64.1 1.0 N B:ASP760 3.7 74.9 1.0 CB B:ASP760 3.9 80.2 1.0 CA B:ASP760 4.0 77.7 1.0 CB B:LEU759 4.0 67.7 1.0 C11 B:CYZ1302 4.0 64.4 1.0 C10 B:CYZ1302 4.1 63.4 1.0 OG C:SER729 4.2 61.3 1.0 OD2 B:ASP760 4.2 78.0 1.0 C B:LEU759 4.3 70.2 1.0 C9 B:CYZ1302 4.6 66.5 1.0 CG B:ASP760 4.6 80.8 1.0 CA B:LEU759 4.7 71.8 1.0 O3 B:CYZ1302 4.9 70.7 1.0 O B:LEU759 4.9 71.2 1.0

Chlorine binding site 3 out of 4 in the Lbd Conformation 2 (LBDCONF2) of GLUA2 Flip Q Isoform of Ampa Receptor in Complex with Gain-of-Function Tarp GAMMA2, with 10MM CACL2, 140MM Nmdg, 330UM Ctz, and 100MM L-Glutamate (Open-CA10)


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 3 of Lbd Conformation 2 (LBDCONF2) of GLUA2 Flip Q Isoform of Ampa Receptor in Complex with Gain-of-Function Tarp GAMMA2, with 10MM CACL2, 140MM Nmdg, 330UM Ctz, and 100MM L-Glutamate (Open-CA10) within 5.0Å range: probe atom residue distance (Å) B Occ C:Cl1302 b:65.5 occ:1.00 CL C:CYZ1302 0.0 65.5 1.0 C13 C:CYZ1302 1.8 39.8 1.0 C12 C:CYZ1302 2.7 41.9 1.0 C14 C:CYZ1302 2.8 37.1 1.0 O4 C:CYZ1302 2.9 57.9 1.0 S2 C:CYZ1302 3.1 55.2 1.0 N C:ASP760 3.3 52.4 1.0 N3 C:CYZ1302 3.4 47.5 1.0 OD1 C:ASP760 3.4 61.5 1.0 CA C:ASP760 3.4 48.5 1.0 CB C:ASP760 3.6 55.5 1.0 CG C:ASP760 3.6 61.0 1.0 C C:LEU759 3.8 44.6 1.0 O C:HOH1401 4.0 0.0 1.0 CB C:LEU759 4.0 48.1 1.0 C11 C:CYZ1302 4.0 39.9 1.0 C10 C:CYZ1302 4.1 36.8 1.0 O C:LEU759 4.2 46.6 1.0 OD2 C:ASP760 4.5 53.1 1.0 CA C:LEU759 4.5 34.1 1.0 C9 C:CYZ1302 4.5 39.0 1.0 O3 C:CYZ1302 4.7 39.8 1.0 C C:ASP760 4.9 49.3 1.0

Chlorine binding site 4 out of 4 in the Lbd Conformation 2 (LBDCONF2) of GLUA2 Flip Q Isoform of Ampa Receptor in Complex with Gain-of-Function Tarp GAMMA2, with 10MM CACL2, 140MM Nmdg, 330UM Ctz, and 100MM L-Glutamate (Open-CA10)


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 4 of Lbd Conformation 2 (LBDCONF2) of GLUA2 Flip Q Isoform of Ampa Receptor in Complex with Gain-of-Function Tarp GAMMA2, with 10MM CACL2, 140MM Nmdg, 330UM Ctz, and 100MM L-Glutamate (Open-CA10) within 5.0Å range: probe atom residue distance (Å) B Occ D:Cl1302 b:91.8 occ:1.00 CL D:CYZ1302 0.0 91.8 1.0 C13 D:CYZ1302 1.8 69.9 1.0 C14 D:CYZ1302 2.7 64.5 1.0 C12 D:CYZ1302 2.8 64.7 1.0 S2 D:CYZ1302 3.2 77.3 1.0 O4 D:CYZ1302 3.2 72.2 1.0 N D:ASP760 3.3 74.2 1.0 N3 D:CYZ1302 3.3 64.5 1.0 CB D:ASP760 3.4 80.0 1.0 CA D:ASP760 3.6 78.9 1.0 CB D:LEU759 3.9 68.8 1.0 OG A:SER729 3.9 58.8 1.0 C D:LEU759 4.0 69.9 1.0 C11 D:CYZ1302 4.0 64.6 1.0 C10 D:CYZ1302 4.1 64.0 1.0 CG D:ASP760 4.2 82.9 1.0 CA D:LEU759 4.5 72.3 1.0 C9 D:CYZ1302 4.5 67.8 1.0 O D:LEU759 4.7 73.5 1.0 OD1 D:ASP760 4.7 82.5 1.0 O3 D:CYZ1302 4.8 74.2 1.0 OD2 D:ASP760 4.8 77.6 1.0 O D:PHE495 4.9 51.9 1.0

T.Nakagawa, X.Wang, F.J.Miguez-Cabello, D.Bowie. The Open Gate of the Ampa Receptor Forms A CA2+ Binding Site Critical in Regulating Ion Transport Nat.Struct.Mol.Biol. 2023.
ISSN: ESSN 1545-9985
DOI: 10.1038/S41594-024-01228-3