Chlorine in PDB 8fsi: The Structure of A Crystallizable Variant of E. Coli Pyruvate Formate- Lyase Activating Enzyme Bound to Sam

Enzymatic activity of The Structure of A Crystallizable Variant of E. Coli Pyruvate Formate- Lyase Activating Enzyme Bound to Sam

All present enzymatic activity of The Structure of A Crystallizable Variant of E. Coli Pyruvate Formate- Lyase Activating Enzyme Bound to Sam:
1.97.1.4;

Protein crystallography data

The structure of The Structure of A Crystallizable Variant of E. Coli Pyruvate Formate- Lyase Activating Enzyme Bound to Sam, PDB code: 8fsi was solved by J.D.Moody, A.Galambas, C.M.Lawrence, J.B.Broderick, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 41.77 / 1.46
Space group P 21 21 21
Cell size a, b, c (Å), α, β, γ (°) 46.405, 59.094, 83.545, 90, 90, 90
R / Rfree (%) 15.1 / 17.3

Other elements in 8fsi:

The structure of The Structure of A Crystallizable Variant of E. Coli Pyruvate Formate- Lyase Activating Enzyme Bound to Sam also contains other interesting chemical elements:

Iron (Fe) 4 atoms
Potassium (K) 2 atoms

Chlorine Binding Sites:

The binding sites of Chlorine atom in the The Structure of A Crystallizable Variant of E. Coli Pyruvate Formate- Lyase Activating Enzyme Bound to Sam (pdb code 8fsi). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total only one binding site of Chlorine was determined in the The Structure of A Crystallizable Variant of E. Coli Pyruvate Formate- Lyase Activating Enzyme Bound to Sam, PDB code: 8fsi:

Chlorine binding site 1 out of 1 in 8fsi

Go back to Chlorine Binding Sites List in 8fsi
Chlorine binding site 1 out of 1 in the The Structure of A Crystallizable Variant of E. Coli Pyruvate Formate- Lyase Activating Enzyme Bound to Sam


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of The Structure of A Crystallizable Variant of E. Coli Pyruvate Formate- Lyase Activating Enzyme Bound to Sam within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Cl304

b:17.6
occ:0.70
H A:LEU30 2.3 17.4 1.0
HD21 A:ASN106 2.5 19.8 1.0
HD2 A:PHE108 2.8 31.1 1.0
HB2 A:LEU30 2.8 22.1 1.0
HG2 A:GLU79 2.8 14.9 1.0
O A:HOH412 2.9 36.4 1.0
HD11 A:LEU82 3.0 25.7 1.0
N A:LEU30 3.1 14.5 1.0
HG A:LEU82 3.1 26.6 1.0
HB3 A:PHE108 3.2 23.1 1.0
HB3 A:GLU79 3.3 15.7 1.0
HD21 A:LEU82 3.3 30.0 1.0
ND2 A:ASN106 3.4 16.5 1.0
HB2 A:GLU79 3.5 15.7 1.0
HG A:LEU30 3.5 21.5 1.0
HA A:CYS29 3.5 15.6 1.0
CG A:GLU79 3.6 12.4 1.0
CB A:LEU30 3.6 18.5 1.0
CB A:GLU79 3.6 13.1 1.0
CD1 A:LEU82 3.7 21.4 1.0
CG A:LEU82 3.7 22.2 1.0
CD2 A:PHE108 3.7 25.9 1.0
CA A:LEU30 3.7 14.5 1.0
H A:MET31 3.8 16.5 1.0
HG3 A:GLU79 3.8 14.9 1.0
HD22 A:ASN106 3.9 19.8 1.0
CD2 A:LEU82 3.9 25.0 1.0
HD12 A:LEU82 4.0 25.7 1.0
HG3 A:MET31 4.0 18.9 1.0
CG A:LEU30 4.0 17.9 1.0
CB A:PHE108 4.1 19.2 1.0
OD1 A:ASN106 4.1 14.1 1.0
HD12 A:LEU30 4.1 27.6 1.0
C A:CYS29 4.2 14.1 1.0
SG A:CYS29 4.2 12.8 1.0
N A:MET31 4.2 13.8 1.0
C A:LEU30 4.2 16.9 1.0
CG A:ASN106 4.2 14.3 1.0
CA A:CYS29 4.3 13.0 1.0
HB2 A:PHE108 4.3 23.1 1.0
HD23 A:LEU82 4.4 30.0 1.0
HB3 A:LEU30 4.4 22.1 1.0
CG A:PHE108 4.4 19.6 1.0
HD13 A:LEU82 4.5 25.7 1.0
HA A:LEU30 4.6 17.4 1.0
CD1 A:LEU30 4.7 23.0 1.0
HE2 A:PHE108 4.7 27.2 1.0
HD22 A:LEU82 4.7 30.0 1.0
HH12 A:ARG110 4.7 48.6 0.5
CE2 A:PHE108 4.7 22.6 1.0
O A:PHE108 4.8 22.7 1.0
CD A:GLU79 4.9 14.1 1.0
HH11 A:ARG110 4.9 48.6 0.5
CB A:CYS29 4.9 11.7 1.0
CG A:MET31 5.0 15.7 1.0
HB2 A:MET31 5.0 17.9 1.0

Reference:

J.D.Moody, S.Hill, M.N.Lundahl, A.J.Saxton, A.Galambas, W.E.Broderick, C.M.Lawrence, J.B.Broderick. Computational Engineering of Previously Crystallized Pyruvate Formate-Lyase Activating Enzyme Reveals Insights Into Sam Binding and Reductive Cleavage. J.Biol.Chem. 04791 2023.
ISSN: ESSN 1083-351X
PubMed: 37156396
DOI: 10.1016/J.JBC.2023.104791
Page generated: Wed Jul 26 16:42:26 2023

Last articles

Zn in 8WB0
Zn in 8WAX
Zn in 8WAU
Zn in 8WAZ
Zn in 8WAY
Zn in 8WAV
Zn in 8WAW
Zn in 8WAT
Zn in 8W7M
Zn in 8WD3
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy