Chlorine in PDB 8ilk: Crystal Structure of A Highly Photostable and Bright Green Fluorescent Protein at PH8.5

The structure of Crystal Structure of A Highly Photostable and Bright Green Fluorescent Protein at PH8.5, PDB code: 8ilk was solved by H.Ago, R.Ando, M.Hirano, S.Shimozono, A.Miyawaki, M.Yamamoto, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	91.22 / 1.56
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	53.876, 44.851, 93.788, 90, 103.44, 90
R / Rfree (%)	18.6 / 21.5

The binding sites of Chlorine atom in the Crystal Structure of A Highly Photostable and Bright Green Fluorescent Protein at PH8.5 (pdb code 8ilk). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 2 binding sites of Chlorine where determined in the Crystal Structure of A Highly Photostable and Bright Green Fluorescent Protein at PH8.5, PDB code: 8ilk:
Jump to Chlorine binding site number: 1; 2;

Chlorine binding site 1 out of 2 in the Crystal Structure of A Highly Photostable and Bright Green Fluorescent Protein at PH8.5


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Crystal Structure of A Highly Photostable and Bright Green Fluorescent Protein at PH8.5 within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl301 b:14.3 occ:1.00 O A:HOH452 3.0 11.8 1.0 NZ A:LYS61 3.1 15.2 1.0 NH2 A:ARG86 3.4 11.7 1.0 NZ A:LYS192 3.6 17.3 1.0 CE A:LYS61 3.7 12.8 1.0 CB2 A:CR258 3.8 16.4 1.0 CD2 A:CR258 3.8 11.7 1.0 OG1 A:THR172 3.9 10.4 1.0 NE2 A:GLN170 3.9 11.7 1.0 CB A:CYS150 3.9 12.6 1.0 CB A:THR172 4.2 11.1 1.0 CG1 A:VAL139 4.2 13.8 1.0 CG2 A:CR258 4.3 12.7 1.0 O2 A:CR258 4.3 13.7 1.0 SG A:CYS150 4.4 14.4 1.0 CZ A:ARG86 4.6 13.6 1.0 CG2 A:THR172 4.6 16.2 1.0 CE A:LYS192 4.7 17.5 1.0 CA2 A:CR258 4.8 13.5 1.0 CE2 A:CR258 4.9 10.9 1.0 O A:CYS150 4.9 13.3 1.0 OH A:TYR84 5.0 13.1 1.0 C2 A:CR258 5.0 11.5 1.0

Chlorine binding site 2 out of 2 in the Crystal Structure of A Highly Photostable and Bright Green Fluorescent Protein at PH8.5


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Crystal Structure of A Highly Photostable and Bright Green Fluorescent Protein at PH8.5 within 5.0Å range: probe atom residue distance (Å) B Occ B:Cl301 b:16.4 occ:1.00 O B:HOH450 3.0 15.3 1.0 NZ B:LYS61 3.1 19.4 1.0 NH2 B:ARG86 3.4 13.4 1.0 NZ B:LYS192 3.6 15.5 1.0 CE B:LYS61 3.7 16.6 1.0 CB2 B:CR258 3.8 17.5 1.0 CB B:CYS150 3.9 15.3 1.0 CD2 B:CR258 3.9 11.6 1.0 OG1 B:THR172 3.9 16.2 1.0 NE2 B:GLN170 3.9 13.2 1.0 CG1 B:VAL139 4.1 16.0 1.0 CB B:THR172 4.2 12.8 1.0 SG B:CYS150 4.4 17.4 1.0 O2 B:CR258 4.4 13.9 1.0 CG2 B:CR258 4.4 16.0 1.0 CZ B:ARG86 4.6 14.0 1.0 CG2 B:THR172 4.6 18.1 1.0 CE B:LYS192 4.7 14.5 1.0 CA2 B:CR258 4.8 11.2 1.0 CE2 B:CR258 4.9 15.0 1.0 NE B:ARG86 5.0 14.3 1.0 OH B:TYR84 5.0 14.1 1.0 O B:CYS150 5.0 14.3 1.0

R.Ando, H.Ago, M.Hirano, S.Shimozono, M.Yamamoto, A.Miyawaki. Crystal Structure of A Highly Photostable and Bright Green Fluorescent Protein. To Be Published.