Chlorine in PDB 8ill: Crystal Structure of A Highly Photostable and Bright Green Fluorescent Protein at PH5.6

The structure of Crystal Structure of A Highly Photostable and Bright Green Fluorescent Protein at PH5.6, PDB code: 8ill was solved by H.Ago, R.Ando, M.Hirano, S.Shimozono, A.Miyawaki, M.Yamamoto, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	67.00 / 2.20
Space group	P 61
Cell size a, b, c (Å), α, β, γ (°)	133.999, 133.999, 59.023, 90, 90, 120
R / Rfree (%)	15.5 / 19

The binding sites of Chlorine atom in the Crystal Structure of A Highly Photostable and Bright Green Fluorescent Protein at PH5.6 (pdb code 8ill). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 2 binding sites of Chlorine where determined in the Crystal Structure of A Highly Photostable and Bright Green Fluorescent Protein at PH5.6, PDB code: 8ill:
Jump to Chlorine binding site number: 1; 2;

Chlorine binding site 1 out of 2 in the Crystal Structure of A Highly Photostable and Bright Green Fluorescent Protein at PH5.6


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Crystal Structure of A Highly Photostable and Bright Green Fluorescent Protein at PH5.6 within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl301 b:11.1 occ:1.00 O A:HOH432 3.0 6.2 1.0 NZ A:LYS61 3.2 7.6 1.0 NH2 A:ARG86 3.4 5.6 1.0 NZ A:LYS192 3.5 6.1 1.0 CE A:LYS61 3.8 8.0 1.0 OG1 A:THR172 3.8 8.6 1.0 CB2 A:CR258 3.9 8.1 1.0 CB A:CYS150 3.9 7.9 1.0 NE2 A:GLN170 3.9 6.5 1.0 CD2 A:CR258 4.0 8.7 1.0 CG1 A:VAL139 4.0 4.7 1.0 CB A:THR172 4.1 9.1 1.0 O2 A:CR258 4.3 10.2 1.0 SG A:CYS150 4.3 9.1 1.0 CG2 A:CR258 4.5 9.2 1.0 CG2 A:THR172 4.5 3.8 1.0 CZ A:ARG86 4.6 6.3 1.0 CE A:LYS192 4.7 6.9 1.0 CA2 A:CR258 4.8 10.0 1.0 OH A:TYR84 4.9 8.9 1.0 NE A:ARG86 5.0 6.4 1.0 C2 A:CR258 5.0 5.2 1.0

Chlorine binding site 2 out of 2 in the Crystal Structure of A Highly Photostable and Bright Green Fluorescent Protein at PH5.6


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Crystal Structure of A Highly Photostable and Bright Green Fluorescent Protein at PH5.6 within 5.0Å range: probe atom residue distance (Å) B Occ B:Cl301 b:9.7 occ:1.00 O B:HOH456 3.0 6.1 1.0 NZ B:LYS61 3.2 5.5 1.0 NH2 B:ARG86 3.4 8.0 1.0 NZ B:LYS192 3.5 6.6 1.0 CE B:LYS61 3.7 6.0 1.0 OG1 B:THR172 3.8 9.4 1.0 CB B:CYS150 3.9 7.1 1.0 NE2 B:GLN170 3.9 5.0 1.0 CB2 B:CR258 3.9 9.0 1.0 CD2 B:CR258 4.0 6.8 1.0 CG1 B:VAL139 4.0 8.5 1.0 CB B:THR172 4.1 7.8 1.0 SG B:CYS150 4.3 10.5 1.0 O2 B:CR258 4.4 7.6 1.0 CG2 B:CR258 4.5 9.5 1.0 CG2 B:THR172 4.5 6.5 1.0 CZ B:ARG86 4.5 14.3 1.0 CE B:LYS192 4.6 6.0 1.0 OH B:TYR84 4.9 9.9 1.0 CA2 B:CR258 4.9 7.5 1.0 NE B:ARG86 4.9 9.6 1.0

R.Ando, H.Ago, M.Hirano, S.Shimozono, M.Yamamoto, A.Miyawaki. Crystal Structure of A Highly Photostable and Bright Green Fluorescent Protein To Be Published.