Chlorine in PDB 8ofb: Crystal Structure of T. Maritima Reverse Gyrase with A Minimal Latch, Hexagonal Form

All present enzymatic activity of Crystal Structure of T. Maritima Reverse Gyrase with A Minimal Latch, Hexagonal Form:
3.6.4.12; 5.6.2.2;

The structure of Crystal Structure of T. Maritima Reverse Gyrase with A Minimal Latch, Hexagonal Form, PDB code: 8ofb was solved by D.Klostermeier, R.Rasche, V.Mhaindarkar, D.Kummel, M.G.Rudolph, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	75.85 / 2.39
Space group	P 61 2 2
Cell size a, b, c (Å), α, β, γ (°)	90.833, 90.833, 571.878, 90, 90, 120
R / Rfree (%)	24.3 / 27.2

The structure of Crystal Structure of T. Maritima Reverse Gyrase with A Minimal Latch, Hexagonal Form also contains other interesting chemical elements:

Zinc

(Zn)

2 atoms

The binding sites of Chlorine atom in the Crystal Structure of T. Maritima Reverse Gyrase with A Minimal Latch, Hexagonal Form (pdb code 8ofb). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 2 binding sites of Chlorine where determined in the Crystal Structure of T. Maritima Reverse Gyrase with A Minimal Latch, Hexagonal Form, PDB code: 8ofb:
Jump to Chlorine binding site number: 1; 2;

Chlorine binding site 1 out of 2 in the Crystal Structure of T. Maritima Reverse Gyrase with A Minimal Latch, Hexagonal Form


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Crystal Structure of T. Maritima Reverse Gyrase with A Minimal Latch, Hexagonal Form within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl1109 b:46.3 occ:1.00 OG A:SER959 3.0 26.3 1.0 O A:HOH1263 3.2 42.6 1.0 CB A:ARG957 3.7 36.9 1.0 CB A:SER959 3.7 28.7 1.0 N A:SER959 4.0 30.0 1.0 CD A:PRO958 4.1 29.1 1.0 CD A:ARG957 4.4 46.4 1.0 N A:PRO958 4.4 28.6 1.0 CG A:ARG957 4.4 47.1 1.0 CA A:SER959 4.5 29.5 1.0 CA A:ARG957 4.8 36.6 1.0 CG2 A:VAL610 4.9 37.3 1.0 C A:ARG957 4.9 30.0 1.0 CG A:PRO958 4.9 30.3 1.0 CB A:PRO958 4.9 29.4 1.0 C A:PRO958 4.9 26.8 1.0 OD2 A:ASP609 5.0 37.5 1.0

Chlorine binding site 2 out of 2 in the Crystal Structure of T. Maritima Reverse Gyrase with A Minimal Latch, Hexagonal Form


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Crystal Structure of T. Maritima Reverse Gyrase with A Minimal Latch, Hexagonal Form within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl1110 b:53.4 occ:1.00 O A:HOH1229 3.6 41.8 1.0 CB A:SER799 3.7 33.5 1.0 N A:LEU800 3.7 33.7 1.0 CA A:SER799 4.0 36.9 1.0 CG A:LEU800 4.3 51.0 1.0 C A:SER799 4.4 33.9 1.0 CB A:LEU800 4.4 33.3 1.0 CA A:LEU800 4.7 30.6 1.0 CD1 A:LEU800 4.7 53.8 1.0 OG A:SER799 4.8 30.1 1.0

V.Mhaindarkar, R.Rasche, D.Kummel, M.G.Rudolph, D.Klostermeier. Structure of Reverse Gyrase with A Minimal Latch That Supports Atp-Dependent Positive Supercoiling Without Specific Interactions with the Topoisomerase Domain Acta Crystallogr.,Sect.D 2023.
ISSN: ESSN 1399-0047
DOI: 10.1107/S2059798323002565