Chlorine in PDB 8pi5: Crystal Structure of Human Insulin DESB30 Precursor with An Alanine- Methionine-Lysine C-Peptide in Hexamer (T3R3) Conformation

The structure of Crystal Structure of Human Insulin DESB30 Precursor with An Alanine- Methionine-Lysine C-Peptide in Hexamer (T3R3) Conformation, PDB code: 8pi5 was solved by E.Johansson, G.Schluckebier, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	13.66 / 1.66
Space group	H 3
Cell size a, b, c (Å), α, β, γ (°)	79.353, 79.353, 39.185, 90, 90, 120
R / Rfree (%)	15.4 / 17.9

The structure of Crystal Structure of Human Insulin DESB30 Precursor with An Alanine- Methionine-Lysine C-Peptide in Hexamer (T3R3) Conformation also contains other interesting chemical elements:

Zinc

(Zn)

4 atoms

The binding sites of Chlorine atom in the Crystal Structure of Human Insulin DESB30 Precursor with An Alanine- Methionine-Lysine C-Peptide in Hexamer (T3R3) Conformation (pdb code 8pi5). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total only one binding site of Chlorine was determined in the Crystal Structure of Human Insulin DESB30 Precursor with An Alanine- Methionine-Lysine C-Peptide in Hexamer (T3R3) Conformation, PDB code: 8pi5:

Chlorine binding site 1 out of 1 in the Crystal Structure of Human Insulin DESB30 Precursor with An Alanine- Methionine-Lysine C-Peptide in Hexamer (T3R3) Conformation


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Crystal Structure of Human Insulin DESB30 Precursor with An Alanine- Methionine-Lysine C-Peptide in Hexamer (T3R3) Conformation within 5.0Å range: probe atom residue distance (Å) B Occ D:Cl102 b:22.4 occ:0.33 ZN D:ZN101 2.0 18.5 0.3 HD13 D:LEU6 3.2 29.5 1.0 HB3 D:LEU6 3.3 22.7 1.0 NE2 D:HIS10 3.5 16.7 1.0 HD2 D:HIS10 3.7 22.1 1.0 CD2 D:HIS10 3.9 18.4 1.0 HB2 D:LEU6 4.0 22.7 1.0 CB D:LEU6 4.1 18.9 1.0 CD1 D:LEU6 4.1 24.6 1.0 HD12 D:LEU6 4.1 29.5 1.0 CE1 D:HIS10 4.6 21.7 1.0 CG D:LEU6 4.7 22.3 1.0 HD11 D:LEU6 4.8 29.5 1.0 HD22 D:LEU6 4.9 27.4 1.0 HE1 D:HIS10 5.0 26.0 1.0

T.Kjeldsen, A.S.Andersen, F.Hubalek, E.Johansson, F.F.Kreiner, G.Schluckebier, P.Kurtzhals. Molecular Engineering of Insulin For Recombinant Expression in Yeast. Trends Biotechnol 2023.
ISSN: ISSN 1879-3096
PubMed: 37880066
DOI: 10.1016/J.TIBTECH.2023.09.012