Chlorine in PDB 8tfy: Trna 2'-Phosphotransferase (TPT1) From Pyrococcus Horikoshii in Complex with Nadp

The structure of Trna 2'-Phosphotransferase (TPT1) From Pyrococcus Horikoshii in Complex with Nadp, PDB code: 8tfy was solved by A.Jacewicz, S.Dantuluri, S.Shuman, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	29.20 / 1.54
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	38.671, 44.531, 124.808, 90, 90, 90
R / Rfree (%)	17 / 19.3

The binding sites of Chlorine atom in the Trna 2'-Phosphotransferase (TPT1) From Pyrococcus Horikoshii in Complex with Nadp (pdb code 8tfy). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 2 binding sites of Chlorine where determined in the Trna 2'-Phosphotransferase (TPT1) From Pyrococcus Horikoshii in Complex with Nadp, PDB code: 8tfy:
Jump to Chlorine binding site number: 1; 2;

Chlorine binding site 1 out of 2 in the Trna 2'-Phosphotransferase (TPT1) From Pyrococcus Horikoshii in Complex with Nadp


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Trna 2'-Phosphotransferase (TPT1) From Pyrococcus Horikoshii in Complex with Nadp within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl202 b:33.2 occ:0.62 O A:HOH326 2.7 29.8 1.0 N A:ARG100 3.1 25.9 1.0 N A:ARG99 3.4 23.4 1.0 CB A:ARG100 3.5 35.6 1.0 CB A:VAL98 3.6 28.9 1.0 CB A:ASP137 3.9 26.2 1.0 CB A:ARG99 3.9 22.9 1.0 CA A:ARG99 3.9 22.2 1.0 CA A:ARG100 3.9 30.1 1.0 O A:ASP137 4.0 26.9 1.0 C A:ARG99 4.0 25.1 1.0 C A:VAL98 4.0 25.0 1.0 CG2 A:VAL98 4.2 29.5 1.0 CA A:VAL98 4.3 23.9 1.0 OD1 A:ASP137 4.4 29.8 1.0 CG1 A:VAL98 4.6 33.6 1.0 CG A:ASP137 4.6 26.3 1.0 C A:ASP137 4.7 25.0 1.0 CA A:ASP137 4.7 24.9 1.0 O A:VAL98 4.8 25.1 1.0 CG A:ARG100 4.8 45.3 1.0 N A:ASP137 5.0 26.1 1.0

Chlorine binding site 2 out of 2 in the Trna 2'-Phosphotransferase (TPT1) From Pyrococcus Horikoshii in Complex with Nadp


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Trna 2'-Phosphotransferase (TPT1) From Pyrococcus Horikoshii in Complex with Nadp within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl203 b:55.7 occ:0.88 O A:HOH360 2.9 30.0 1.0 O3B A:NAP201 3.6 24.6 1.0 O A:HOH369 3.7 38.1 1.0 C3B A:NAP201 3.8 23.5 1.0 O A:HOH348 4.0 34.6 1.0 NH1 A:ARG133 4.0 32.4 1.0 CD A:ARG133 4.0 23.5 1.0 C4B A:NAP201 4.2 26.0 1.0 C5B A:NAP201 4.5 29.8 1.0 NE A:ARG133 4.7 26.3 1.0 CZ A:ARG133 4.7 30.0 1.0

A.Jacewicz, S.Dantuluri, S.Shuman. Structural Basis For TPT1-Catalyzed 2'-Po 4 Transfer From Rna and Nadp(H) to Nad. Proc.Natl.Acad.Sci.Usa V. 120 99120 2023.
ISSN: ESSN 1091-6490
PubMed: 37883434
DOI: 10.1073/PNAS.2312999120