Chlorine in PDB 8tfz: Trna 2'-Phosphotransferase (TPT1) From Pyrococcus Horikoshii in Complex with Nad

The structure of Trna 2'-Phosphotransferase (TPT1) From Pyrococcus Horikoshii in Complex with Nad, PDB code: 8tfz was solved by A.Jacewicz, S.Dantuluri, S.Shuman, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	37.63 / 2.06
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	55.759, 44.585, 72.142, 90, 103.48, 90
R / Rfree (%)	22.9 / 28.7

The binding sites of Chlorine atom in the Trna 2'-Phosphotransferase (TPT1) From Pyrococcus Horikoshii in Complex with Nad (pdb code 8tfz). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 3 binding sites of Chlorine where determined in the Trna 2'-Phosphotransferase (TPT1) From Pyrococcus Horikoshii in Complex with Nad, PDB code: 8tfz:
Jump to Chlorine binding site number: 1; 2; 3;

Chlorine binding site 1 out of 3 in the Trna 2'-Phosphotransferase (TPT1) From Pyrococcus Horikoshii in Complex with Nad


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Trna 2'-Phosphotransferase (TPT1) From Pyrococcus Horikoshii in Complex with Nad within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl203 b:46.4 occ:1.00 NE2 A:HIS15 2.9 24.4 1.0 NH2 A:ARG62 3.4 37.7 1.0 O A:HOH311 3.5 30.8 1.0 CD2 A:HIS15 3.7 25.0 1.0 O A:HOH319 3.8 32.6 1.0 CE1 A:HIS15 3.9 24.7 1.0 NH1 A:ARG62 4.0 37.8 1.0 CZ A:ARG62 4.1 38.2 1.0 O2 A:GOL202 4.1 30.9 0.8 CB A:ALA10 4.3 25.2 1.0 CB A:ALA14 4.5 23.3 1.0 O A:GLY75 4.5 23.8 1.0 C1 A:GOL202 4.9 30.9 0.8 CG A:HIS15 4.9 26.6 1.0 ND1 A:HIS15 5.0 26.2 1.0

Chlorine binding site 2 out of 3 in the Trna 2'-Phosphotransferase (TPT1) From Pyrococcus Horikoshii in Complex with Nad


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Trna 2'-Phosphotransferase (TPT1) From Pyrococcus Horikoshii in Complex with Nad within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl204 b:30.9 occ:0.54 OE2 A:GLU89 2.5 59.0 1.0 CD A:GLU89 3.4 58.8 1.0 OE1 A:GLU89 3.7 60.8 1.0 NZ A:LYS91 4.6 51.6 1.0 CG A:GLU89 4.6 57.4 1.0

Chlorine binding site 3 out of 3 in the Trna 2'-Phosphotransferase (TPT1) From Pyrococcus Horikoshii in Complex with Nad


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 3 of Trna 2'-Phosphotransferase (TPT1) From Pyrococcus Horikoshii in Complex with Nad within 5.0Å range: probe atom residue distance (Å) B Occ B:Cl203 b:30.0 occ:0.87 NE2 B:HIS15 2.8 25.6 1.0 NH2 B:ARG62 3.1 35.3 1.0 O B:HOH347 3.3 24.0 1.0 O B:HOH329 3.3 27.1 1.0 CE1 B:HIS15 3.8 26.0 1.0 CD2 B:HIS15 3.8 24.9 1.0 CZ B:ARG62 4.1 35.9 1.0 NH1 B:ARG62 4.4 36.2 1.0 CB B:ALA10 4.4 24.0 1.0 O B:GLY75 4.4 25.8 1.0 C1 B:GOL202 4.4 20.9 1.0 CB B:ALA14 4.7 23.7 1.0 ND1 B:HIS15 4.9 25.7 1.0 CG B:HIS15 4.9 25.0 1.0

A.Jacewicz, S.Dantuluri, S.Shuman. Structural Basis For TPT1-Catalyzed 2'-Po 4 Transfer From Rna and Nadp(H) to Nad. Proc.Natl.Acad.Sci.Usa V. 120 99120 2023.
ISSN: ESSN 1091-6490
PubMed: 37883434
DOI: 10.1073/PNAS.2312999120