Chlorine in PDB 8tys: Adaptive Mechanism of Collagen IV Scaffold Assembly in Drosophila: Crystal Structure of Tissue-Extracted NC1 Hexamer

The structure of Adaptive Mechanism of Collagen IV Scaffold Assembly in Drosophila: Crystal Structure of Tissue-Extracted NC1 Hexamer, PDB code: 8tys was solved by S.P.Boudko, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	40.63 / 2.90
Space group	P 63
Cell size a, b, c (Å), α, β, γ (°)	145.209, 145.209, 106.491, 90, 90, 120
R / Rfree (%)	24.1 / 27.7

The structure of Adaptive Mechanism of Collagen IV Scaffold Assembly in Drosophila: Crystal Structure of Tissue-Extracted NC1 Hexamer also contains other interesting chemical elements:

Calcium

(Ca)

2 atoms

The binding sites of Chlorine atom in the Adaptive Mechanism of Collagen IV Scaffold Assembly in Drosophila: Crystal Structure of Tissue-Extracted NC1 Hexamer (pdb code 8tys). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 4 binding sites of Chlorine where determined in the Adaptive Mechanism of Collagen IV Scaffold Assembly in Drosophila: Crystal Structure of Tissue-Extracted NC1 Hexamer, PDB code: 8tys:
Jump to Chlorine binding site number: 1; 2; 3; 4;

Chlorine binding site 1 out of 4 in the Adaptive Mechanism of Collagen IV Scaffold Assembly in Drosophila: Crystal Structure of Tissue-Extracted NC1 Hexamer


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Adaptive Mechanism of Collagen IV Scaffold Assembly in Drosophila: Crystal Structure of Tissue-Extracted NC1 Hexamer within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl301 b:69.8 occ:1.00 O A:ARG76 3.2 75.8 1.0 C A:ARG76 3.2 72.4 1.0 N A:ASP78 3.3 64.4 1.0 N A:ARG76 3.3 72.8 1.0 N A:ASN77 3.7 70.2 1.0 CA A:ARG76 3.7 72.1 1.0 CA A:ALA74 3.8 77.7 1.0 CB A:ALA177 3.9 83.4 1.0 CB A:ASP78 3.9 62.7 1.0 N A:SER75 4.0 73.6 1.0 O A:ASP78 4.0 73.8 1.0 C A:ALA74 4.0 76.3 1.0 CA A:ASP78 4.1 62.9 1.0 N A:ALA177 4.1 80.9 1.0 C A:ASN77 4.2 65.1 1.0 CA A:ASN77 4.2 69.8 1.0 CB A:ARG76 4.2 70.2 1.0 CA A:ALA177 4.4 83.3 1.0 CG A:ASP78 4.4 65.3 1.0 CG A:LEU60 4.4 73.6 1.0 C A:ASP78 4.5 66.9 1.0 C A:SER75 4.5 73.0 1.0 N A:ALA74 4.5 78.7 1.0 OD2 A:ASP78 4.5 66.4 1.0 O A:ALA74 4.6 79.2 1.0 CD2 A:LEU60 4.7 68.6 1.0 CA A:SER75 4.8 71.1 1.0 C A:GLY176 4.9 85.6 1.0 CD1 A:LEU60 4.9 74.5 1.0 CB A:ALA74 4.9 80.3 1.0

Chlorine binding site 2 out of 4 in the Adaptive Mechanism of Collagen IV Scaffold Assembly in Drosophila: Crystal Structure of Tissue-Extracted NC1 Hexamer


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Adaptive Mechanism of Collagen IV Scaffold Assembly in Drosophila: Crystal Structure of Tissue-Extracted NC1 Hexamer within 5.0Å range: probe atom residue distance (Å) B Occ C:Cl301 b:57.4 occ:1.00 N C:ARG76 3.2 59.1 1.0 C C:ARG76 3.2 63.9 1.0 O C:ARG76 3.4 72.3 1.0 CD2 C:LEU60 3.4 59.1 1.0 N C:ASP78 3.4 55.5 1.0 N C:ASN77 3.5 61.2 1.0 CA C:ARG76 3.6 59.9 1.0 CA C:ALA74 3.7 62.3 1.0 N C:SER75 3.8 57.8 1.0 C C:ALA74 3.8 60.5 1.0 CB C:ALA177 4.1 53.3 1.0 CA C:ASN77 4.1 58.5 1.0 CB C:ARG76 4.1 60.0 1.0 CB C:ASP78 4.2 56.3 1.0 C C:ASN77 4.3 58.9 1.0 C C:SER75 4.3 59.1 1.0 CA C:ASP78 4.3 58.0 1.0 NH2 E:ARG181 4.3 60.7 1.0 O C:ASP78 4.3 68.2 1.0 N C:ALA177 4.4 64.8 1.0 O C:ALA74 4.4 61.7 1.0 N C:ALA74 4.5 65.8 1.0 CG C:ASP78 4.5 58.1 1.0 CA C:SER75 4.6 58.2 1.0 OD2 C:ASP78 4.6 58.8 1.0 CA C:ALA177 4.7 62.4 1.0 CB C:ALA74 4.7 61.8 1.0 C C:ASP78 4.8 62.8 1.0 CG C:LEU60 4.8 55.9 1.0

Chlorine binding site 3 out of 4 in the Adaptive Mechanism of Collagen IV Scaffold Assembly in Drosophila: Crystal Structure of Tissue-Extracted NC1 Hexamer


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 3 of Adaptive Mechanism of Collagen IV Scaffold Assembly in Drosophila: Crystal Structure of Tissue-Extracted NC1 Hexamer within 5.0Å range: probe atom residue distance (Å) B Occ D:Cl301 b:85.1 occ:1.00 N D:ARG76 3.1 82.9 1.0 C D:ARG76 3.4 79.2 1.0 O D:ARG76 3.5 84.9 1.0 N D:SER75 3.6 84.7 1.0 CA D:ALA74 3.6 86.2 1.0 CA D:ARG76 3.6 79.5 1.0 N D:ASP78 3.7 64.9 1.0 C D:ALA74 3.8 88.6 1.0 CD2 D:LEU60 3.8 76.1 1.0 N D:ASN77 3.9 75.2 1.0 CB D:ARG76 3.9 77.1 1.0 CB D:ASP78 4.0 72.6 1.0 N D:ALA74 4.1 84.7 1.0 OD2 D:ASP78 4.1 83.3 1.0 CG D:ASP78 4.1 80.5 1.0 C D:SER75 4.2 81.3 1.0 CA D:ASP78 4.4 64.8 1.0 CB D:ALA177 4.5 78.0 1.0 CA D:SER75 4.5 85.0 1.0 CD1 D:LEU60 4.6 73.5 1.0 CA D:ASN77 4.6 73.9 1.0 O D:ALA74 4.6 92.0 1.0 C D:ASN77 4.6 72.3 1.0 O D:ASP78 4.6 79.8 1.0 CB D:ALA74 4.8 89.1 1.0 CG D:LEU60 4.8 75.7 1.0 N D:ALA177 4.9 83.3 1.0 OD1 D:ASP78 4.9 80.2 1.0 C D:ASP78 5.0 72.0 1.0

Chlorine binding site 4 out of 4 in the Adaptive Mechanism of Collagen IV Scaffold Assembly in Drosophila: Crystal Structure of Tissue-Extracted NC1 Hexamer


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 4 of Adaptive Mechanism of Collagen IV Scaffold Assembly in Drosophila: Crystal Structure of Tissue-Extracted NC1 Hexamer within 5.0Å range: probe atom residue distance (Å) B Occ F:Cl301 b:54.5 occ:1.00 C F:ARG76 3.2 45.6 1.0 N F:ARG76 3.2 47.5 1.0 N F:ASP78 3.3 49.7 1.0 O F:ARG76 3.4 49.7 1.0 N F:ASN77 3.4 42.5 1.0 CD2 F:LEU60 3.6 54.5 1.0 CA F:ARG76 3.7 45.6 1.0 CA F:ALA74 3.8 48.8 1.0 N F:SER75 3.8 47.8 1.0 C F:ALA74 3.9 50.2 1.0 NH2 B:ARG181 4.0 49.0 1.0 CA F:ASN77 4.0 47.1 1.0 CB F:ASP78 4.0 51.2 1.0 C F:ASN77 4.1 49.2 1.0 CA F:ASP78 4.1 49.9 1.0 CB F:ARG76 4.2 39.8 1.0 CB F:ALA177 4.2 49.5 1.0 O F:ASP78 4.3 57.5 1.0 C F:SER75 4.4 46.4 1.0 CG F:ASP78 4.4 56.3 1.0 OD2 F:ASP78 4.5 56.0 1.0 N F:ALA177 4.5 59.1 1.0 N F:ALA74 4.6 50.4 1.0 O F:ALA74 4.6 56.0 1.0 C F:ASP78 4.7 54.7 1.0 CA F:SER75 4.7 47.1 1.0 CB F:ALA74 4.8 52.2 1.0 CA F:ALA177 4.8 53.9 1.0 CG F:LEU60 4.9 55.3 1.0

J.A.Summers, M.Yarbrough, M.Liu, W.H.Mcdonald, B.G.Hudson, J.C.Pastor-Pareja, S.P.Boudko. Collagen IV of Basement Membranes: IV. Adaptive Mechanism of Collagen IV Scaffold Assembly in Drosophila. J.Biol.Chem. 05394 2023.
ISSN: ESSN 1083-351X
PubMed: 37890775
DOI: 10.1016/J.JBC.2023.105394