Chlorine in PDB 8up8: Structure of Atypical Asparaginase From Rhodospirillum Rubrum (Mutant Y21F, Complex with L-Asp)

The structure of Structure of Atypical Asparaginase From Rhodospirillum Rubrum (Mutant Y21F, Complex with L-Asp), PDB code: 8up8 was solved by J.Lubkowski, A.Wlodawer, D.Zhang, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	38.97 / 1.90
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	72.145, 77.64, 115.183, 90, 90, 90
R / Rfree (%)	17.8 / 22.5

The binding sites of Chlorine atom in the Structure of Atypical Asparaginase From Rhodospirillum Rubrum (Mutant Y21F, Complex with L-Asp) (pdb code 8up8). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 2 binding sites of Chlorine where determined in the Structure of Atypical Asparaginase From Rhodospirillum Rubrum (Mutant Y21F, Complex with L-Asp), PDB code: 8up8:
Jump to Chlorine binding site number: 1; 2;

Chlorine binding site 1 out of 2 in the Structure of Atypical Asparaginase From Rhodospirillum Rubrum (Mutant Y21F, Complex with L-Asp)


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Structure of Atypical Asparaginase From Rhodospirillum Rubrum (Mutant Y21F, Complex with L-Asp) within 5.0Å range: probe atom residue distance (Å) B Occ B:Cl202 b:39.7 occ:1.00 N C:LEU28 3.4 16.0 1.0 N B:LEU28 3.5 18.0 1.0 CB C:LEU28 3.9 16.9 1.0 CG C:LEU28 4.0 14.9 1.0 CB B:LEU28 4.0 19.3 1.0 CA C:GLY27 4.1 17.4 1.0 CA B:GLY27 4.1 22.2 1.0 CG B:LEU28 4.1 18.1 1.0 CD1 C:LEU28 4.3 15.6 1.0 CA C:LEU28 4.3 16.5 1.0 C C:GLY27 4.3 15.6 1.0 C B:GLY27 4.3 18.6 1.0 CA B:LEU28 4.3 18.3 1.0 CD1 B:LEU28 4.5 18.4 1.0 O C:HOH313 4.5 34.4 1.0 O C:LEU28 4.6 18.8 1.0 O B:LEU28 4.7 20.0 1.0 C C:LEU28 5.0 17.6 1.0

Chlorine binding site 2 out of 2 in the Structure of Atypical Asparaginase From Rhodospirillum Rubrum (Mutant Y21F, Complex with L-Asp)


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Structure of Atypical Asparaginase From Rhodospirillum Rubrum (Mutant Y21F, Complex with L-Asp) within 5.0Å range: probe atom residue distance (Å) B Occ D:Cl201 b:50.1 occ:1.00 N D:LEU28 3.5 27.2 1.0 N A:LEU28 3.6 23.7 1.0 CB D:LEU28 4.0 23.8 1.0 CB A:LEU28 4.1 27.1 1.0 CA D:GLY27 4.1 26.1 1.0 CG D:LEU28 4.1 24.9 1.0 CG A:LEU28 4.1 25.3 1.0 O D:HOH315 4.2 40.7 1.0 CA A:GLY27 4.2 29.2 1.0 C D:GLY27 4.3 23.0 1.0 CA D:LEU28 4.4 24.4 1.0 CD1 A:LEU28 4.4 28.2 1.0 C A:GLY27 4.5 24.4 1.0 CA A:LEU28 4.5 25.8 1.0 CD1 D:LEU28 4.5 23.4 1.0 O D:LEU28 4.7 29.8 1.0 O A:LEU28 4.8 24.5 1.0

D.Zhang, H.Czapinska, M.Bochtler, A.Wlodawer, J.Lubkowski. Rra, An Enzyme From Rhodospirillum Rubrum, Is A Prototype of A New Family of Short-Chain L-Asparaginases. Protein Sci. V. 33 E4920 2024.
ISSN: ESSN 1469-896X
PubMed: 38501449
DOI: 10.1002/PRO.4920