Chlorine in PDB 8vuc: Structure of FABS1CE2-Epr-1, An Elbow-Locked High Affinity Antibody For the Erythropoeitin Receptor

The structure of Structure of FABS1CE2-Epr-1, An Elbow-Locked High Affinity Antibody For the Erythropoeitin Receptor, PDB code: 8vuc was solved by A.U.Singer, H.A.Bruce, A.Pavlenco, L.Ploder, G.Luu, L.Blazer, J.J.Adams, S.S.Sidhu, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	55.14 / 2.50
Space group	P 32
Cell size a, b, c (Å), α, β, γ (°)	67.389, 67.389, 168.232, 90, 90, 120
R / Rfree (%)	20.4 / 26.5

The binding sites of Chlorine atom in the Structure of FABS1CE2-Epr-1, An Elbow-Locked High Affinity Antibody For the Erythropoeitin Receptor (pdb code 8vuc). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 4 binding sites of Chlorine where determined in the Structure of FABS1CE2-Epr-1, An Elbow-Locked High Affinity Antibody For the Erythropoeitin Receptor, PDB code: 8vuc:
Jump to Chlorine binding site number: 1; 2; 3; 4;

Chlorine binding site 1 out of 4 in the Structure of FABS1CE2-Epr-1, An Elbow-Locked High Affinity Antibody For the Erythropoeitin Receptor


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Structure of FABS1CE2-Epr-1, An Elbow-Locked High Affinity Antibody For the Erythropoeitin Receptor within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl305 b:116.3 occ:1.00 CD2 A:PHE160 3.0 62.9 1.0 CE2 A:PHE160 3.1 64.0 1.0 N A:ASN128 3.4 82.6 1.0 O A:GLN129 3.6 58.0 1.0 CG A:PHE160 3.7 59.2 1.0 CB A:ASN128 3.7 83.2 1.0 OD1 A:ASN128 3.7 91.7 1.0 N A:GLN129 3.7 70.7 1.0 CZ A:PHE160 3.9 63.7 1.0 CG A:ASN128 3.9 87.0 1.0 CA A:ASN128 4.0 79.2 1.0 CD1 A:PHE160 4.4 58.1 1.0 CB A:PHE160 4.4 54.6 1.0 C A:ASN128 4.4 74.7 1.0 C A:PHE127 4.4 79.0 1.0 CE1 A:PHE160 4.5 59.9 1.0 CA A:PHE127 4.5 76.3 1.0 C A:GLN129 4.6 63.5 1.0 O A:GLY188 4.8 63.6 1.0 CA A:GLN129 4.8 66.3 1.0 ND2 A:ASN128 4.8 84.0 1.0 CB A:PHE127 4.9 71.3 1.0 CD2 B:TYR62 4.9 106.9 1.0

Chlorine binding site 2 out of 4 in the Structure of FABS1CE2-Epr-1, An Elbow-Locked High Affinity Antibody For the Erythropoeitin Receptor


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Structure of FABS1CE2-Epr-1, An Elbow-Locked High Affinity Antibody For the Erythropoeitin Receptor within 5.0Å range: probe atom residue distance (Å) B Occ G:Cl302 b:82.8 occ:1.00 N G:GLU231 3.2 91.3 1.0 N G:GLY230 3.7 79.5 1.0 O G:GLU231 3.8 99.5 1.0 CA G:GLU231 3.9 96.4 1.0 O G:ASN228 3.9 77.5 1.0 CB G:GLU231 4.0 93.0 1.0 C G:GLU231 4.0 99.7 1.0 C G:GLY230 4.1 87.6 1.0 CA G:GLY230 4.1 82.0 1.0 CG G:PRO139 4.2 52.1 1.0 CD2 G:PHE227 4.3 60.6 1.0 CB G:PRO139 4.3 50.7 1.0 C G:ARG229 4.7 77.3 1.0 CE2 G:PHE227 4.7 57.0 1.0 CA G:ARG229 4.9 72.8 1.0 N G:CYS232 4.9 102.3 1.0 C G:ASN228 5.0 73.5 1.0

Chlorine binding site 3 out of 4 in the Structure of FABS1CE2-Epr-1, An Elbow-Locked High Affinity Antibody For the Erythropoeitin Receptor


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 3 of Structure of FABS1CE2-Epr-1, An Elbow-Locked High Affinity Antibody For the Erythropoeitin Receptor within 5.0Å range: probe atom residue distance (Å) B Occ G:Cl303 b:113.0 occ:1.00 N G:GLY47 2.6 77.6 1.0 CA G:PRO46 3.4 75.5 1.0 C G:PRO46 3.5 75.7 1.0 CA G:GLY47 3.5 74.2 1.0 N G:LYS48 4.2 69.6 1.0 O G:LYS45 4.2 70.3 1.0 NE2 G:GLN44 4.3 58.6 1.0 OE1 G:GLN44 4.3 56.8 1.0 C G:GLY47 4.3 72.2 1.0 CD G:GLN44 4.4 60.2 1.0 OE1 A:GLN44 4.4 51.4 1.0 N G:PRO46 4.4 73.1 1.0 CB G:PRO46 4.5 74.8 1.0 C G:LYS45 4.6 71.0 1.0 O G:PRO46 4.6 70.5 1.0 O G:LYS48 4.6 67.4 1.0 O A:HOH403 4.7 56.4 1.0

Chlorine binding site 4 out of 4 in the Structure of FABS1CE2-Epr-1, An Elbow-Locked High Affinity Antibody For the Erythropoeitin Receptor


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 4 of Structure of FABS1CE2-Epr-1, An Elbow-Locked High Affinity Antibody For the Erythropoeitin Receptor within 5.0Å range: probe atom residue distance (Å) B Occ B:Cl301 b:112.2 occ:1.00 OD1 B:ASN128 3.1 90.1 1.0 CD1 B:PHE160 3.5 61.8 1.0 CE1 B:PHE160 3.6 65.8 1.0 N B:ASN128 3.6 81.3 1.0 CG B:ASN128 3.7 85.3 1.0 O B:GLN129 3.7 62.0 1.0 CG B:PHE160 3.8 57.8 1.0 CB B:ASN128 3.8 82.2 1.0 N B:GLN129 3.8 67.0 1.0 CZ B:PHE160 3.9 65.8 1.0 CD2 B:PHE160 4.1 57.9 1.0 CE2 B:PHE160 4.1 62.0 1.0 CA B:ASN128 4.1 76.9 1.0 C B:ASN128 4.5 75.0 1.0 CB B:PHE160 4.5 53.6 1.0 C B:PHE127 4.6 79.0 1.0 O B:GLY188 4.6 65.2 1.0 C B:GLN129 4.7 66.3 1.0 CA B:PHE127 4.7 75.6 1.0 ND2 B:ASN128 4.8 80.4 1.0 CA B:GLN129 4.8 64.2 1.0

H.A.Bruce, A.U.Singer, L.L.Blazer, K.Luu, L.Ploder, A.Pavlenco, I.Kurinov, J.J.Adams, S.S.Sidhu. Antigen-Binding Fragments with Improved Crystal Lattice Packing and Enhanced Conformational Flexibility at the Elbow Region As Crystallization Chaperones. Protein Sci. V. 33 E5081 2024.
ISSN: ESSN 1469-896X
PubMed: 38924648
DOI: 10.1002/PRO.5081