Chlorine in PDB 8zur: Crystal Structure of the F99S/M153T/V163A/T203V/E222Q Variant of Gfp at pH 5.0

The structure of Crystal Structure of the F99S/M153T/V163A/T203V/E222Q Variant of Gfp at pH 5.0, PDB code: 8zur was solved by R.Takeda, K.Takeda, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	41.20 / 1.20
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	51.5, 57.79, 68.67, 90, 90, 90
R / Rfree (%)	12.9 / 16.6

The binding sites of Chlorine atom in the Crystal Structure of the F99S/M153T/V163A/T203V/E222Q Variant of Gfp at pH 5.0 (pdb code 8zur). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 2 binding sites of Chlorine where determined in the Crystal Structure of the F99S/M153T/V163A/T203V/E222Q Variant of Gfp at pH 5.0, PDB code: 8zur:
Jump to Chlorine binding site number: 1; 2;

Chlorine binding site 1 out of 2 in the Crystal Structure of the F99S/M153T/V163A/T203V/E222Q Variant of Gfp at pH 5.0


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Crystal Structure of the F99S/M153T/V163A/T203V/E222Q Variant of Gfp at pH 5.0 within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl301 b:14.2 occ:1.00 O A:HOH409 3.2 12.8 1.0 O A:HOH429 3.2 18.9 1.0 N A:ASN149 3.4 11.4 1.0 NH2 A:ARG168 3.4 13.1 1.0 CA A:HIS148 3.7 12.0 1.0 CB A:HIS148 4.0 11.9 1.0 C A:HIS148 4.1 11.2 1.0 O A:ASN149 4.3 12.8 1.0 CD2 A:HIS148 4.3 11.2 1.0 CA A:ASN149 4.4 12.0 1.0 CB A:ASN149 4.4 13.2 1.0 CZ A:ARG168 4.5 12.4 1.0 CG A:HIS148 4.5 10.8 1.0 NE A:ARG168 4.6 12.5 1.0 O A:SER147 4.6 13.3 1.0 O A:LYS166 4.6 11.6 1.0 CG A:ASN149 4.8 14.5 1.0 C A:ASN149 4.8 12.0 1.0 OH A:TYR151 5.0 15.6 1.0 N A:HIS148 5.0 11.8 1.0 ND2 A:ASN149 5.0 15.9 1.0

Chlorine binding site 2 out of 2 in the Crystal Structure of the F99S/M153T/V163A/T203V/E222Q Variant of Gfp at pH 5.0


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Crystal Structure of the F99S/M153T/V163A/T203V/E222Q Variant of Gfp at pH 5.0 within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl302 b:16.6 occ:1.00 O A:HOH470 2.8 11.9 0.3 O A:HOH470 2.9 18.7 0.7 O A:HOH495 3.0 12.8 1.0 O A:HOH500 3.0 9.9 0.3 N A:TRP57 3.1 9.4 1.0 O A:HOH512 3.3 19.5 1.0 O A:HOH500 3.3 16.2 0.7 CD1 A:TRP57 3.6 9.9 1.0 O A:HOH546 3.6 17.8 0.5 CA A:PRO56 3.7 10.1 1.0 CB A:TRP57 3.7 10.0 1.0 CD A:PRO58 3.8 9.1 1.0 C A:PRO56 3.8 9.5 1.0 CD1 A:TYR143 3.9 10.6 1.0 CA A:TRP57 4.0 9.4 1.0 CG A:TRP57 4.0 9.4 1.0 O A:HOH546 4.1 23.1 0.5 CB A:PRO56 4.1 10.8 1.0 O A:HOH553 4.4 24.1 0.3 CE1 A:TYR143 4.4 10.6 1.0 O A:HOH494 4.7 17.7 1.0 O A:HOH553 4.7 20.6 0.7 N A:PRO58 4.7 8.8 1.0 NE1 A:TRP57 4.8 9.9 1.0 O A:VAL55 4.8 10.8 1.0 CG A:PRO58 4.9 9.9 1.0 C A:TRP57 4.9 8.7 1.0 CG A:TYR143 4.9 10.5 1.0 N A:PRO56 5.0 10.4 1.0

R.Takeda, E.Tsutsumi, K.Okatsu, S.Fukai, K.Takeda. Structural Characterization of Green Fluorescent Protein in the I-State. Sci Rep V. 14 22832 2024.
ISSN: ESSN 2045-2322
PubMed: 39353998
DOI: 10.1038/S41598-024-73696-Y