Chlorine in PDB 9fcg: Medicago Truncatula 5'-Profar Isomerase (HISN3) D57N Mutant in Complex with Prfar

All present enzymatic activity of Medicago Truncatula 5'-Profar Isomerase (HISN3) D57N Mutant in Complex with Prfar:
5.3.1.16;

The structure of Medicago Truncatula 5'-Profar Isomerase (HISN3) D57N Mutant in Complex with Prfar, PDB code: 9fcg was solved by W.Witek, B.Imiolczyk, M.Ruszkowski, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	35.63 / 1.54
Space group	P 4
Cell size a, b, c (Å), α, β, γ (°)	87.79, 87.79, 35.633, 90, 90, 90
R / Rfree (%)	17.4 / 19.8

The structure of Medicago Truncatula 5'-Profar Isomerase (HISN3) D57N Mutant in Complex with Prfar also contains other interesting chemical elements:

Sodium

(Na)

2 atoms

The binding sites of Chlorine atom in the Medicago Truncatula 5'-Profar Isomerase (HISN3) D57N Mutant in Complex with Prfar (pdb code 9fcg). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 2 binding sites of Chlorine where determined in the Medicago Truncatula 5'-Profar Isomerase (HISN3) D57N Mutant in Complex with Prfar, PDB code: 9fcg:
Jump to Chlorine binding site number: 1; 2;

Chlorine binding site 1 out of 2 in the Medicago Truncatula 5'-Profar Isomerase (HISN3) D57N Mutant in Complex with Prfar


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Medicago Truncatula 5'-Profar Isomerase (HISN3) D57N Mutant in Complex with Prfar within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl408 b:20.5 occ:1.00 O A:HOH533 3.1 23.7 1.0 N A:GLY165 3.2 18.4 1.0 O A:HOH666 3.3 20.5 1.0 O A:HOH696 3.4 28.0 1.0 N A:ASN164 3.6 18.1 1.0 CA A:ASN163 3.6 17.7 1.0 C A:ASN163 3.7 20.4 1.0 NZ A:LYS206 3.8 31.9 1.0 N A:ASN163 3.8 14.0 1.0 C A:PHE162 3.8 15.0 1.0 CA A:GLY165 3.8 19.1 1.0 O A:PHE162 3.8 15.9 1.0 CE A:LYS206 3.9 33.1 1.0 O A:HOH649 3.9 31.8 1.0 CB A:PHE162 4.0 17.6 1.0 C A:ASN164 4.2 21.3 1.0 NA A:NA406 4.3 19.0 1.0 CD A:LYS206 4.4 32.5 1.0 CA A:ASN164 4.4 17.8 1.0 O A:ASN163 4.5 18.3 1.0 CA A:PHE162 4.5 14.8 1.0 OG1 A:THR201 4.6 19.1 1.0 C A:GLY165 4.7 19.1 1.0 OD2 A:ASP202 4.7 21.9 1.0 N A:GLN166 4.9 16.8 1.0 O A:HOH752 5.0 27.4 1.0

Chlorine binding site 2 out of 2 in the Medicago Truncatula 5'-Profar Isomerase (HISN3) D57N Mutant in Complex with Prfar


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Medicago Truncatula 5'-Profar Isomerase (HISN3) D57N Mutant in Complex with Prfar within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl409 b:26.6 occ:1.00 O A:HOH668 3.0 30.9 1.0 N A:ASN294 3.2 17.6 1.0 O A:HOH519 3.3 23.2 1.0 CA A:GLY293 3.8 18.6 1.0 CA A:ALA287 3.8 15.2 1.0 CB A:ASN294 3.9 21.4 1.0 O A:ALA287 3.9 16.9 1.0 CB A:ALA287 4.0 14.2 1.0 CD A:LYS238 4.0 27.0 1.0 C A:GLY293 4.0 20.8 1.0 ND2 A:ASN294 4.0 31.0 1.0 CA A:ASN294 4.1 20.2 1.0 CG2 A:THR264 4.2 18.6 1.0 CG A:ASN294 4.2 27.8 1.0 CE A:LYS238 4.2 29.4 1.0 C A:ALA287 4.3 14.3 1.0 NZ A:LYS238 4.5 33.5 1.0 CG A:LYS238 4.5 20.0 1.0 N A:LEU295 4.6 15.6 1.0 C A:ASN294 4.8 22.0 1.0 O A:HOH627 4.8 35.8 1.0 O A:THR264 4.9 18.9 1.0 CB A:LYS238 4.9 16.3 1.0 O A:SER286 4.9 16.3 1.0 N A:ALA287 5.0 14.5 1.0

W.Witek, B.Imiolczyk, M.Ruszkowski. Structural, Kinetic, and Evolutionary Peculiarities of HISN3, A Plant 5'-Profar Isomerase. Plant Physiol Biochem. V. 215 09065 2024.
ISSN: ESSN 1873-2690
PubMed: 39186852
DOI: 10.1016/J.PLAPHY.2024.109065