Chlorine in PDB 9h8q: Eugenol Oxidase (Eugo)From Rhodococcus Jostii RHA1, Mutant Dtt-T425G

All present enzymatic activity of Eugenol Oxidase (Eugo)From Rhodococcus Jostii RHA1, Mutant Dtt-T425G:
1.1.3.38;

The structure of Eugenol Oxidase (Eugo)From Rhodococcus Jostii RHA1, Mutant Dtt-T425G, PDB code: 9h8q was solved by H.J.Rozeboom, M.W.Fraaije, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	102.75 / 1.60
Space group	P 1
Cell size a, b, c (Å), α, β, γ (°)	90.439, 110.197, 117.536, 89.87, 89.34, 68.51
R / Rfree (%)	17.7 / 19.8

The binding sites of Chlorine atom in the Eugenol Oxidase (Eugo)From Rhodococcus Jostii RHA1, Mutant Dtt-T425G (pdb code 9h8q). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 2 binding sites of Chlorine where determined in the Eugenol Oxidase (Eugo)From Rhodococcus Jostii RHA1, Mutant Dtt-T425G, PDB code: 9h8q:
Jump to Chlorine binding site number: 1; 2;

Chlorine binding site 1 out of 2 in the Eugenol Oxidase (Eugo)From Rhodococcus Jostii RHA1, Mutant Dtt-T425G


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Eugenol Oxidase (Eugo)From Rhodococcus Jostii RHA1, Mutant Dtt-T425G within 5.0Å range: probe atom residue distance (Å) B Occ B:Cl603 b:25.2 occ:1.00 N B:GLN187 3.2 14.8 1.0 O A:HOH738 3.2 24.4 1.0 N B:GLU189 3.3 18.4 1.0 CB B:GLN187 3.4 16.8 1.0 N B:GLY188 3.5 14.8 1.0 CA B:GLN187 3.5 15.3 1.0 CB B:GLU189 3.5 23.9 1.0 CB B:LEU185 3.5 15.3 1.0 C B:GLN187 3.5 16.0 1.0 CG B:GLU189 3.7 27.6 1.0 C B:LEU185 3.9 14.4 1.0 CA B:GLU189 4.0 19.8 1.0 O B:LEU185 4.0 14.1 1.0 N B:PRO186 4.1 15.1 1.0 CA B:LEU185 4.2 14.1 1.0 O B:GLN187 4.3 16.0 1.0 C B:GLY188 4.4 17.9 1.0 C B:PRO186 4.4 14.7 1.0 CG B:GLN187 4.4 18.5 1.0 CA B:GLY188 4.5 15.6 1.0 O B:GLU189 4.5 20.7 1.0 O A:HOH1063 4.7 37.2 1.0 CD1 B:LEU185 4.7 18.1 1.0 CG B:LEU185 4.7 17.7 1.0 C B:GLU189 4.7 18.0 1.0 CD B:PRO186 4.7 13.8 1.0 CA B:PRO186 4.8 14.0 1.0 N B:LEU185 4.8 13.3 1.0 OE1 B:GLN187 4.9 25.9 1.0

Chlorine binding site 2 out of 2 in the Eugenol Oxidase (Eugo)From Rhodococcus Jostii RHA1, Mutant Dtt-T425G


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Eugenol Oxidase (Eugo)From Rhodococcus Jostii RHA1, Mutant Dtt-T425G within 5.0Å range: probe atom residue distance (Å) B Occ F:Cl605 b:23.0 occ:1.00 O F:HOH880 3.1 21.7 1.0 N F:GLN187 3.2 15.1 1.0 N F:GLU189 3.3 17.1 1.0 CB F:LEU185 3.5 13.9 1.0 N F:GLY188 3.5 14.8 1.0 CB F:GLN187 3.5 17.0 1.0 CA F:GLN187 3.6 15.5 1.0 CB F:GLU189 3.6 22.3 1.0 C F:GLN187 3.6 15.9 1.0 C F:LEU185 3.8 13.6 1.0 CG F:GLU189 3.9 26.1 1.0 O F:LEU185 3.9 13.2 1.0 CA F:GLU189 4.0 18.8 1.0 N F:PRO186 4.0 14.1 1.0 CA F:LEU185 4.2 13.3 1.0 O E:HOH1088 4.3 34.7 1.0 C F:GLY188 4.3 16.6 1.0 C F:PRO186 4.4 15.5 1.0 O F:GLN187 4.4 15.4 1.0 CG F:GLN187 4.5 17.7 1.0 O F:GLU189 4.5 19.0 1.0 CA F:GLY188 4.5 15.2 1.0 CD F:PRO186 4.6 15.7 1.0 C F:GLU189 4.7 16.9 1.0 CG F:LEU185 4.7 15.8 1.0 CD1 F:LEU185 4.7 16.0 1.0 CA F:PRO186 4.8 15.1 1.0 N F:LEU185 4.8 13.0 1.0 OE1 F:GLN187 4.9 23.2 1.0

B.Ashley, C.Demingo, H.J.Rozeboom, N.Bianciardi, M.W.Fraaije. Biocatalytic Cleavage of Para-Acetoxy Benzyl Ethers: Application to Protecting Group Chemistry To Be Published.