Chlorine in PDB 191l: A Helix Initiation Signal in T4 Lysozyme Identified By Polyalanine Mutagenesis

All present enzymatic activity of A Helix Initiation Signal in T4 Lysozyme Identified By Polyalanine Mutagenesis:
3.2.1.17;

The structure of A Helix Initiation Signal in T4 Lysozyme Identified By Polyalanine Mutagenesis, PDB code: 191l was solved by X.-J.Zhang, B.W.Matthews, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	20.00 / 1.95
Space group	P 32 2 1
Cell size a, b, c (Å), α, β, γ (°)	61.000, 61.000, 97.200, 90.00, 90.00, 120.00
R / Rfree (%)	n/a / n/a

The binding sites of Chlorine atom in the A Helix Initiation Signal in T4 Lysozyme Identified By Polyalanine Mutagenesis (pdb code 191l). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 3 binding sites of Chlorine where determined in the A Helix Initiation Signal in T4 Lysozyme Identified By Polyalanine Mutagenesis, PDB code: 191l:
Jump to Chlorine binding site number: 1; 2; 3;

Chlorine binding site 1 out of 3 in the A Helix Initiation Signal in T4 Lysozyme Identified By Polyalanine Mutagenesis


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of A Helix Initiation Signal in T4 Lysozyme Identified By Polyalanine Mutagenesis within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl173 b:31.2 occ:1.00 O A:HOH209 3.0 30.8 1.0 N A:ARG145 3.3 13.3 1.0 N A:ASN144 3.4 7.4 1.0 C A:THR142 3.5 21.6 1.0 CA A:THR142 3.6 5.6 1.0 CB A:THR142 3.7 22.7 1.0 O A:THR142 3.7 17.0 1.0 CB A:ASN144 3.7 13.9 1.0 N A:PRO143 3.9 21.1 1.0 CA A:ASN144 3.9 10.2 1.0 CB A:ARG145 4.0 23.4 1.0 C A:ASN144 4.1 17.9 1.0 CA A:ARG145 4.2 26.9 1.0 CG2 A:THR142 4.3 11.9 1.0 C A:PRO143 4.3 16.5 1.0 CD A:PRO143 4.4 16.5 1.0 CA A:PRO143 4.7 13.0 1.0 O A:HOH230 4.7 41.0 1.0 CG A:ASN144 4.7 29.1 1.0 OG1 A:THR142 4.9 17.3 1.0 ND2 A:ASN144 4.9 24.9 1.0 N A:THR142 5.0 9.3 1.0

Chlorine binding site 2 out of 3 in the A Helix Initiation Signal in T4 Lysozyme Identified By Polyalanine Mutagenesis


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of A Helix Initiation Signal in T4 Lysozyme Identified By Polyalanine Mutagenesis within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl178 b:33.7 occ:0.50 O A:HOH215 2.9 25.9 1.0 C A:LYS135 3.8 18.9 1.0 N A:SER136 4.0 11.2 1.0 OD1 A:ASN140 4.1 23.3 1.0 CA A:LYS135 4.1 18.0 1.0 O A:LYS135 4.1 22.3 1.0 CB A:LYS135 4.4 22.1 1.0 CA A:SER136 4.6 16.0 1.0 O A:HOH282 4.8 30.7 1.0 C A:SER136 4.8 14.5 1.0 O A:SER136 5.0 16.8 1.0

Chlorine binding site 3 out of 3 in the A Helix Initiation Signal in T4 Lysozyme Identified By Polyalanine Mutagenesis


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 3 of A Helix Initiation Signal in T4 Lysozyme Identified By Polyalanine Mutagenesis within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl197 b:22.8 occ:1.00 OG A:SER117 3.1 18.6 1.0 N A:SER117 3.1 18.2 1.0 N A:ASN116 3.5 24.5 1.0 C A:PHE114 3.5 21.0 1.0 CA A:PHE114 3.6 13.5 1.0 O A:PHE114 3.7 18.3 1.0 CB A:ASN116 3.7 26.1 1.0 CB A:SER117 3.8 11.0 1.0 CA A:ASN116 3.8 19.1 1.0 C A:ASN116 3.9 18.9 1.0 CB A:PHE114 4.0 13.1 1.0 N A:THR115 4.0 13.5 1.0 CA A:SER117 4.0 22.8 1.0 C A:THR115 4.4 25.7 1.0 CD2 A:PHE114 4.4 44.7 1.0 CG A:PHE114 4.5 21.0 1.0 ND2 A:ASN116 4.5 79.4 1.0 CB A:ALA132 4.5 14.9 1.0 O A:ALA132 4.7 17.2 1.0 CG A:ASN116 4.7 34.7 1.0 CA A:THR115 4.7 18.5 1.0 O A:HOH314 4.7 36.2 1.0 O A:HOH312 4.7 44.4 1.0 N A:PHE114 4.9 17.3 1.0 O A:GLY113 4.9 30.8 1.0

X.J.Zhang, W.A.Baase, B.W.Matthews. A Helix Initiation Signal in T4 Lysozyme Identified By Polyalanine Mutagenesis. Biophys.Chem. V.-102 43 2002.
ISSN: ISSN 0301-4622
PubMed: 12487988
DOI: 10.1016/S0301-4622(02)00193-X