Chlorine in PDB 1b0i: Alpha-Amylase From Alteromonas Haloplanctis

All present enzymatic activity of Alpha-Amylase From Alteromonas Haloplanctis:
3.2.1.1;

The structure of Alpha-Amylase From Alteromonas Haloplanctis, PDB code: 1b0i was solved by N.Aghajari, R.Haser, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	20.00 / 2.40
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	56.800, 80.300, 120.100, 90.00, 90.00, 90.00
R / Rfree (%)	18 / 21.7

The structure of Alpha-Amylase From Alteromonas Haloplanctis also contains other interesting chemical elements:

Calcium

(Ca)

1 atom

The binding sites of Chlorine atom in the Alpha-Amylase From Alteromonas Haloplanctis (pdb code 1b0i). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total only one binding site of Chlorine was determined in the Alpha-Amylase From Alteromonas Haloplanctis, PDB code: 1b0i:

Chlorine binding site 1 out of 1 in the Alpha-Amylase From Alteromonas Haloplanctis


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Alpha-Amylase From Alteromonas Haloplanctis within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl900 b:23.2 occ:1.00 HZ2 A:LYS300 2.3 15.0 1.0 HE A:ARG172 2.5 15.0 1.0 H1 A:HOH1003 2.5 0.0 1.0 HH21 A:ARG172 2.6 0.0 1.0 HZ1 A:LYS300 3.0 15.0 1.0 HD22 A:ASN262 3.0 0.0 1.0 NZ A:LYS300 3.1 1.8 1.0 NE A:ARG172 3.4 10.4 1.0 NH2 A:ARG172 3.5 14.5 1.0 O A:HOH1003 3.5 21.0 1.0 H2 A:HOH1003 3.9 0.0 1.0 CG2 A:THR221 3.9 8.4 1.0 HZ3 A:LYS300 3.9 15.0 1.0 CE A:LYS300 3.9 7.4 1.0 CZ A:ARG172 3.9 12.3 1.0 ND2 A:ASN262 4.0 9.1 1.0 CE2 A:PHE259 4.2 14.0 1.0 HH22 A:ARG172 4.3 0.0 1.0 H2 A:HOH1002 4.4 0.0 1.0 CZ A:PHE223 4.4 16.6 1.0 CZ A:PHE259 4.4 15.4 1.0 CB A:ASN262 4.5 11.5 1.0 CD A:ARG172 4.5 9.9 1.0 O A:HOH1002 4.6 18.6 1.0 HE21 A:GLN33 4.6 0.0 1.0 HD21 A:ASN262 4.6 0.0 1.0 CG A:GLU200 4.6 15.7 1.0 CE2 A:PHE198 4.7 17.7 1.0 CB A:THR221 4.7 11.1 1.0 CG A:ASN262 4.8 16.9 1.0 CD2 A:PHE259 4.8 11.2 1.0 CE1 A:PHE223 4.8 17.9 1.0 CB A:GLU200 4.9 19.1 1.0

N.Aghajari, G.Feller, C.Gerday, R.Haser. Structures of the Psychrophilic Alteromonas Haloplanctis Alpha-Amylase Give Insights Into Cold Adaptation at A Molecular Level. Structure V. 6 1503 1998.
ISSN: ISSN 0969-2126
PubMed: 9862804
DOI: 10.1016/S0969-2126(98)00149-X