Chlorine in PDB 1c9v: H12A Variant of Ribonuclease A

All present enzymatic activity of H12A Variant of Ribonuclease A:
3.1.27.5;

The structure of H12A Variant of Ribonuclease A, PDB code: 1c9v was solved by C.Park, L.W.Schultz, R.T.Raines, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	30.00 / 1.70
Space group	P 32 2 1
Cell size a, b, c (Å), α, β, γ (°)	64.540, 64.540, 65.080, 90.00, 90.00, 120.00
R / Rfree (%)	n/a / n/a

The binding sites of Chlorine atom in the H12A Variant of Ribonuclease A (pdb code 1c9v). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 2 binding sites of Chlorine where determined in the H12A Variant of Ribonuclease A, PDB code: 1c9v:
Jump to Chlorine binding site number: 1; 2;

Chlorine binding site 1 out of 2 in the H12A Variant of Ribonuclease A


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of H12A Variant of Ribonuclease A within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl160 b:35.9 occ:1.00 OG1 A:THR3 3.0 20.9 1.0 CB A:ALA5 3.9 17.5 1.0 CB A:THR3 3.9 20.5 1.0 CG2 A:THR3 4.0 22.3 1.0 O A:HOH209 4.0 39.6 1.0 N A:ALA6 4.3 19.6 1.0 CB A:ALA6 4.4 14.3 1.0 O A:HOH149 4.7 30.4 1.0 CA A:ALA6 4.9 15.2 1.0 C A:ALA5 4.9 19.8 1.0 CA A:ALA5 4.9 14.3 1.0 O A:HOH159 4.9 33.3 1.0

Chlorine binding site 2 out of 2 in the H12A Variant of Ribonuclease A


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of H12A Variant of Ribonuclease A within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl172 b:29.2 occ:1.00 ND2 A:ASN34 3.1 29.1 1.0 O A:HOH155 3.2 28.8 1.0 CD A:ARG10 3.5 18.5 1.0 O A:HOH175 3.5 67.4 1.0 NE A:ARG10 3.8 21.7 1.0 CG A:ASN34 4.1 34.8 1.0 CB A:ASN34 4.1 26.0 1.0 CZ A:ARG10 4.3 22.3 1.0 NH1 A:ARG10 4.4 22.6 1.0 O A:ARG33 4.7 23.2 1.0 CG A:ARG10 4.9 17.2 1.0

C.Park, L.W.Schultz, R.T.Raines. Contribution of the Active Site Histidine Residues of Ribonuclease A to Nucleic Acid Binding. Biochemistry V. 40 4949 2001.
ISSN: ISSN 0006-2960
PubMed: 11305910
DOI: 10.1021/BI0100182