Chlorine in PDB 1c9x: H119A Variant of Ribonuclease A

The structure of H119A Variant of Ribonuclease A, PDB code: 1c9x was solved by C.Park, L.W.Schultz, R.T.Raines, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	30.00 / 1.80
Space group	P 32 2 1
Cell size a, b, c (Å), α, β, γ (°)	64.530, 64.530, 65.040, 90.00, 90.00, 120.00
R / Rfree (%)	n/a / n/a

The binding sites of Chlorine atom in the H119A Variant of Ribonuclease A (pdb code 1c9x). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 3 binding sites of Chlorine where determined in the H119A Variant of Ribonuclease A, PDB code: 1c9x:
Jump to Chlorine binding site number: 1; 2; 3;

Chlorine binding site 1 out of 3 in the H119A Variant of Ribonuclease A


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of H119A Variant of Ribonuclease A within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl160 b:29.9 occ:1.00 OG1 A:THR3 3.0 17.0 1.0 CB A:THR3 4.0 16.2 1.0 CB A:ALA5 4.0 13.3 1.0 CG2 A:THR3 4.1 19.9 1.0 O A:HOH236 4.1 34.7 1.0 N A:ALA6 4.2 12.7 1.0 CB A:ALA6 4.4 12.5 1.0 CA A:ALA6 4.7 11.4 1.0 O A:HOH149 4.8 23.4 1.0 O A:HOH159 4.8 29.8 1.0 C A:ALA5 4.9 15.9 1.0 CA A:ALA5 4.9 13.4 1.0

Chlorine binding site 2 out of 3 in the H119A Variant of Ribonuclease A


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of H119A Variant of Ribonuclease A within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl172 b:30.9 occ:1.00 ND2 A:ASN34 3.1 28.7 1.0 O A:HOH155 3.2 25.0 1.0 CD A:ARG10 3.5 16.2 1.0 NE A:ARG10 3.9 14.8 1.0 CG A:ASN34 4.0 26.1 1.0 CB A:ASN34 4.1 28.0 1.0 CZ A:ARG10 4.3 20.6 1.0 NH1 A:ARG10 4.4 18.8 1.0 O A:ARG33 4.7 23.1 1.0 CG A:ARG10 4.9 14.8 1.0

Chlorine binding site 3 out of 3 in the H119A Variant of Ribonuclease A


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 3 of H119A Variant of Ribonuclease A within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl195 b:27.5 occ:1.00 O A:HOH211 3.2 48.8 1.0 N A:THR45 3.2 11.7 1.0 CE1 A:HIS12 3.5 10.2 1.0 CA A:ASN44 3.6 14.7 1.0 OG1 A:THR45 3.7 17.3 1.0 C A:ASN44 3.8 16.4 1.0 CD2 A:PHE120 3.8 25.3 1.0 CB A:THR45 4.0 18.1 1.0 ND1 A:HIS12 4.2 11.4 1.0 CA A:THR45 4.2 9.1 1.0 CB A:PHE120 4.3 12.3 1.0 O A:VAL43 4.3 17.9 1.0 OD1 A:ASN44 4.3 21.2 1.0 CG A:PHE120 4.3 18.2 1.0 N A:ASN44 4.3 12.2 1.0 NE2 A:HIS12 4.5 13.2 1.0 CE2 A:PHE120 4.6 23.6 1.0 C A:VAL43 4.6 17.5 1.0 NZ A:LYS41 4.6 34.1 1.0 O A:PHE120 4.7 24.8 1.0 CG1 A:VAL43 4.7 18.6 1.0 CG A:ASN44 4.8 19.2 1.0 CB A:ASN44 4.8 11.9 1.0 O A:THR45 4.8 16.1 1.0 O A:ASN44 4.9 20.7 1.0

C.Park, L.W.Schultz, R.T.Raines. Contribution of the Active Site Histidine Residues of Ribonuclease A to Nucleic Acid Binding. Biochemistry V. 40 4949 2001.
ISSN: ISSN 0006-2960
PubMed: 11305910
DOI: 10.1021/BI0100182