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Chlorine in PDB 1deh: Crystallization of Human BETA1 Alcohol Dehydrogenase (15 Mg/Ml) in 50 Mm Sodium Phosphate (pH 7.5), 2.0 Mm Nad+ and 1 Mm 4-Iodopyrazole at 25 Oc, 13% (W/V) Peg 8000
Enzymatic activity of Crystallization of Human BETA1 Alcohol Dehydrogenase (15 Mg/Ml) in 50 Mm Sodium Phosphate (pH 7.5), 2.0 Mm Nad+ and 1 Mm 4-Iodopyrazole at 25 Oc, 13% (W/V) Peg 8000
All present enzymatic activity of Crystallization of Human BETA1 Alcohol Dehydrogenase (15 Mg/Ml) in 50 Mm Sodium Phosphate (pH 7.5), 2.0 Mm Nad+ and 1 Mm 4-Iodopyrazole at 25 Oc, 13% (W/V) Peg 8000:
1.1.1.1;
1.1.1.1;
Protein crystallography data
The structure of Crystallization of Human BETA1 Alcohol Dehydrogenase (15 Mg/Ml) in 50 Mm Sodium Phosphate (pH 7.5), 2.0 Mm Nad+ and 1 Mm 4-Iodopyrazole at 25 Oc, 13% (W/V) Peg 8000, PDB code: 1deh
was solved by
T.D.Hurley,
G.J.Davis,
with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:
| Resolution Low / High (Å) | 8.00 / 2.20 |
| Space group | P 1 |
| Cell size a, b, c (Å), α, β, γ (°) | 54.040, 44.540, 93.320, 92.56, 103.26, 68.84 |
| R / Rfree (%) | 18 / 26.1 |
Other elements in 1deh:
The structure of Crystallization of Human BETA1 Alcohol Dehydrogenase (15 Mg/Ml) in 50 Mm Sodium Phosphate (pH 7.5), 2.0 Mm Nad+ and 1 Mm 4-Iodopyrazole at 25 Oc, 13% (W/V) Peg 8000 also contains other interesting chemical elements:
| Iodine | (I) | 2 atoms |
| Zinc | (Zn) | 4 atoms |
Chlorine Binding Sites:
The binding sites of Chlorine atom in the Crystallization of Human BETA1 Alcohol Dehydrogenase (15 Mg/Ml) in 50 Mm Sodium Phosphate (pH 7.5), 2.0 Mm Nad+ and 1 Mm 4-Iodopyrazole at 25 Oc, 13% (W/V) Peg 8000
(pdb code 1deh). This binding sites where shown within
5.0 Angstroms radius around Chlorine atom.
In total only one binding site of Chlorine was determined in the Crystallization of Human BETA1 Alcohol Dehydrogenase (15 Mg/Ml) in 50 Mm Sodium Phosphate (pH 7.5), 2.0 Mm Nad+ and 1 Mm 4-Iodopyrazole at 25 Oc, 13% (W/V) Peg 8000, PDB code: 1deh:
In total only one binding site of Chlorine was determined in the Crystallization of Human BETA1 Alcohol Dehydrogenase (15 Mg/Ml) in 50 Mm Sodium Phosphate (pH 7.5), 2.0 Mm Nad+ and 1 Mm 4-Iodopyrazole at 25 Oc, 13% (W/V) Peg 8000, PDB code: 1deh:
Chlorine binding site 1 out of 1 in 1deh
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Chlorine Binding Sites List in 1deh
Chlorine binding site 1 out
of 1 in the Crystallization of Human BETA1 Alcohol Dehydrogenase (15 Mg/Ml) in 50 Mm Sodium Phosphate (pH 7.5), 2.0 Mm Nad+ and 1 Mm 4-Iodopyrazole at 25 Oc, 13% (W/V) Peg 8000
Mono view
Stereo pair view
Mono view
Stereo pair view
A full contact list of Chlorine with other atoms in the Cl binding
site number 1 of Crystallization of Human BETA1 Alcohol Dehydrogenase (15 Mg/Ml) in 50 Mm Sodium Phosphate (pH 7.5), 2.0 Mm Nad+ and 1 Mm 4-Iodopyrazole at 25 Oc, 13% (W/V) Peg 8000 within 5.0Å range:
|
Reference:
G.J.Davis,
W.F.Bosron,
C.L.Stone,
K.Owusu-Dekyi,
T.D.Hurley.
X-Ray Structure of Human BETA3BETA3 Alcohol Dehydrogenase. the Contribution of Ionic Interactions to Coenzyme Binding. J.Biol.Chem. V. 271 17057 1996.
ISSN: ISSN 0021-9258
PubMed: 8663387
DOI: 10.1074/JBC.271.29.17057
Page generated: Sat Dec 12 08:33:46 2020
ISSN: ISSN 0021-9258
PubMed: 8663387
DOI: 10.1074/JBC.271.29.17057
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