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Chlorine in PDB 1g94: Crystal Structure Analysis of the Ternary Complex Between Psychrophilic Alpha Amylase From Pseudoalteromonas Haloplanctis in Complex with A Hepta-Saccharide and A Tris Molecule

Enzymatic activity of Crystal Structure Analysis of the Ternary Complex Between Psychrophilic Alpha Amylase From Pseudoalteromonas Haloplanctis in Complex with A Hepta-Saccharide and A Tris Molecule

All present enzymatic activity of Crystal Structure Analysis of the Ternary Complex Between Psychrophilic Alpha Amylase From Pseudoalteromonas Haloplanctis in Complex with A Hepta-Saccharide and A Tris Molecule:
3.2.1.1;

Protein crystallography data

The structure of Crystal Structure Analysis of the Ternary Complex Between Psychrophilic Alpha Amylase From Pseudoalteromonas Haloplanctis in Complex with A Hepta-Saccharide and A Tris Molecule, PDB code: 1g94 was solved by N.Aghajari, M.Roth, R.Haser, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 44.70 / 1.74
Space group C 2 2 21
Cell size a, b, c (Å), α, β, γ (°) 71.010, 139.960, 115.890, 90.00, 90.00, 90.00
R / Rfree (%) 15.6 / 18.7

Other elements in 1g94:

The structure of Crystal Structure Analysis of the Ternary Complex Between Psychrophilic Alpha Amylase From Pseudoalteromonas Haloplanctis in Complex with A Hepta-Saccharide and A Tris Molecule also contains other interesting chemical elements:

Calcium (Ca) 1 atom

Chlorine Binding Sites:

The binding sites of Chlorine atom in the Crystal Structure Analysis of the Ternary Complex Between Psychrophilic Alpha Amylase From Pseudoalteromonas Haloplanctis in Complex with A Hepta-Saccharide and A Tris Molecule (pdb code 1g94). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total only one binding site of Chlorine was determined in the Crystal Structure Analysis of the Ternary Complex Between Psychrophilic Alpha Amylase From Pseudoalteromonas Haloplanctis in Complex with A Hepta-Saccharide and A Tris Molecule, PDB code: 1g94:

Chlorine binding site 1 out of 1 in 1g94

Go back to Chlorine Binding Sites List in 1g94
Chlorine binding site 1 out of 1 in the Crystal Structure Analysis of the Ternary Complex Between Psychrophilic Alpha Amylase From Pseudoalteromonas Haloplanctis in Complex with A Hepta-Saccharide and A Tris Molecule


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Crystal Structure Analysis of the Ternary Complex Between Psychrophilic Alpha Amylase From Pseudoalteromonas Haloplanctis in Complex with A Hepta-Saccharide and A Tris Molecule within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Cl900

b:12.8
occ:1.00
O A:HOH1003 3.1 12.0 1.0
NE A:ARG172 3.1 9.4 1.0
NZ A:LYS300 3.3 9.0 1.0
NH1 A:ARG172 3.4 10.7 1.0
ND2 A:ASN262 3.4 12.9 1.0
CZ A:ARG172 3.7 11.7 1.0
CG2 A:THR221 4.0 10.8 1.0
CE A:LYS300 4.0 11.1 1.0
CD A:ARG172 4.2 10.9 1.0
CG A:GLU200 4.3 7.7 1.0
CG A:ASN262 4.4 12.8 1.0
CB A:ASN262 4.5 10.7 1.0
CZ A:PHE223 4.5 12.2 1.0
CE1 A:PHE198 4.6 16.9 1.0
CE2 A:PHE259 4.6 12.2 1.0
CB A:GLU200 4.7 11.6 1.0
CZ A:PHE259 4.8 12.9 1.0
CE2 A:PHE223 5.0 9.7 1.0

Reference:

N.Aghajari, M.Roth, R.Haser. Crystallographic Evidence of A Transglycosylation Reaction: Ternary Complexes of A Psychrophilic Alpha-Amylase. Biochemistry V. 41 4273 2002.
ISSN: ISSN 0006-2960
PubMed: 11914073
DOI: 10.1021/BI0160516
Page generated: Sat Dec 12 08:35:55 2020

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