Chlorine in PDB 1h80: 1,3-Alpha-1,4-Beta-D-Galactose-4-Sulfate- 3,6-Anhydro-D-Galactose-2- Sulfate 4 Galactohydrolase

All present enzymatic activity of 1,3-Alpha-1,4-Beta-D-Galactose-4-Sulfate- 3,6-Anhydro-D-Galactose-2- Sulfate 4 Galactohydrolase:
3.2.1.157;

The structure of 1,3-Alpha-1,4-Beta-D-Galactose-4-Sulfate- 3,6-Anhydro-D-Galactose-2- Sulfate 4 Galactohydrolase, PDB code: 1h80 was solved by G.Michel, L.Chantalat, O.Dideberg, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	20.00 / 1.60
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	55.870, 90.070, 124.120, 90.00, 93.53, 90.00
R / Rfree (%)	20.7 / 22.3

The structure of 1,3-Alpha-1,4-Beta-D-Galactose-4-Sulfate- 3,6-Anhydro-D-Galactose-2- Sulfate 4 Galactohydrolase also contains other interesting chemical elements:

Calcium	(Ca)	6 atoms
Sodium	(Na)	6 atoms

The binding sites of Chlorine atom in the 1,3-Alpha-1,4-Beta-D-Galactose-4-Sulfate- 3,6-Anhydro-D-Galactose-2- Sulfate 4 Galactohydrolase (pdb code 1h80). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 2 binding sites of Chlorine where determined in the 1,3-Alpha-1,4-Beta-D-Galactose-4-Sulfate- 3,6-Anhydro-D-Galactose-2- Sulfate 4 Galactohydrolase, PDB code: 1h80:
Jump to Chlorine binding site number: 1; 2;

Chlorine binding site 1 out of 2 in the 1,3-Alpha-1,4-Beta-D-Galactose-4-Sulfate- 3,6-Anhydro-D-Galactose-2- Sulfate 4 Galactohydrolase


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of 1,3-Alpha-1,4-Beta-D-Galactose-4-Sulfate- 3,6-Anhydro-D-Galactose-2- Sulfate 4 Galactohydrolase within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl1495 b:28.3 occ:1.00 NE2 A:GLN222 2.8 14.7 1.0 N A:ALA185 2.9 13.6 1.0 O A:HOH2296 3.0 38.1 1.0 CD2 A:LEU188 3.4 16.0 1.0 CD1 A:TYR218 3.4 16.4 1.0 CB A:ALA185 3.6 14.3 1.0 OH A:TYR224 3.6 21.4 1.0 CA A:PHE184 3.7 14.8 1.0 O A:HOH2294 3.8 47.2 1.0 C A:PHE184 3.8 13.6 1.0 CE1 A:TYR218 3.8 17.8 1.0 O A:HOH2155 3.8 49.4 1.0 CA A:ALA185 3.8 13.8 1.0 CD A:GLN222 3.9 15.9 1.0 CB A:GLN222 4.1 13.7 1.0 O A:HOH2293 4.1 18.6 1.0 CD1 A:PHE184 4.2 19.2 1.0 CB A:PHE184 4.3 16.6 1.0 CG A:GLN222 4.5 15.0 1.0 CG A:TYR218 4.6 15.4 1.0 O A:ALA185 4.7 14.1 1.0 N A:PHE184 4.7 15.7 1.0 OE1 A:GLN222 4.7 18.6 1.0 CG A:PHE184 4.8 17.7 1.0 C A:ALA185 4.8 13.5 1.0 CZ A:TYR224 4.8 20.3 1.0 CG A:LEU188 4.8 16.1 1.0 O A:PHE184 5.0 13.6 1.0

Chlorine binding site 2 out of 2 in the 1,3-Alpha-1,4-Beta-D-Galactose-4-Sulfate- 3,6-Anhydro-D-Galactose-2- Sulfate 4 Galactohydrolase


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of 1,3-Alpha-1,4-Beta-D-Galactose-4-Sulfate- 3,6-Anhydro-D-Galactose-2- Sulfate 4 Galactohydrolase within 5.0Å range: probe atom residue distance (Å) B Occ B:Cl1495 b:28.0 occ:1.00 NE2 B:GLN222 2.9 13.2 1.0 O B:HOH2349 2.9 31.1 1.0 N B:ALA185 3.0 11.6 1.0 CD1 B:TYR218 3.4 14.1 1.0 CD2 B:LEU188 3.5 14.3 1.0 OH B:TYR224 3.6 20.2 1.0 CB B:ALA185 3.7 12.3 1.0 CA B:PHE184 3.7 13.3 1.0 CE1 B:TYR218 3.8 15.8 1.0 C B:PHE184 3.8 12.0 1.0 CD B:GLN222 3.9 14.8 1.0 CA B:ALA185 3.9 11.5 1.0 O B:HOH2188 4.0 40.5 1.0 CB B:GLN222 4.1 13.2 1.0 CD1 B:PHE184 4.2 18.6 1.0 O B:HOH2344 4.3 18.4 1.0 CB B:PHE184 4.4 14.9 1.0 CG B:GLN222 4.5 14.1 1.0 CG B:TYR218 4.6 13.2 1.0 N B:PHE184 4.7 13.9 1.0 O B:ALA185 4.7 12.2 1.0 CZ B:TYR224 4.8 18.3 1.0 CG B:PHE184 4.8 16.8 1.0 OE1 B:GLN222 4.8 17.8 1.0 O B:HOH2350 4.8 30.8 1.0 C B:ALA185 4.9 11.4 1.0 CG B:LEU188 4.9 13.8 1.0 CB B:TYR218 5.0 12.1 1.0

G.Michel, L.Chantalat, E.Fanchon, B.Henrissat, B.Kloareg, O.Dideberg. The Iota-Carrageenase of Alteromonas Fortis. A Beta-Helix Fold-Containing Enzyme For the Degradation of A Highly Polyanionic Polysaccharide J.Biol.Chem. V. 276 40202 2001.
ISSN: ISSN 0021-9258
PubMed: 11493601
DOI: 10.1074/JBC.M100670200