Chlorine in PDB 1k5c: Endopolygalacturonase I From Stereum Purpureum at 0.96 A Resolution

All present enzymatic activity of Endopolygalacturonase I From Stereum Purpureum at 0.96 A Resolution:
3.2.1.15;

The structure of Endopolygalacturonase I From Stereum Purpureum at 0.96 A Resolution, PDB code: 1k5c was solved by T.Shimizu, T.Nakatsu, K.Miyairi, T.Okuno, H.Kato, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	10.00 / 0.96
Space group	P 1
Cell size a, b, c (Å), α, β, γ (°)	46.396, 52.023, 37.344, 72.50, 68.93, 69.24
R / Rfree (%)	11.4 / 14

The binding sites of Chlorine atom in the Endopolygalacturonase I From Stereum Purpureum at 0.96 A Resolution (pdb code 1k5c). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total only one binding site of Chlorine was determined in the Endopolygalacturonase I From Stereum Purpureum at 0.96 A Resolution, PDB code: 1k5c:

Chlorine binding site 1 out of 1 in the Endopolygalacturonase I From Stereum Purpureum at 0.96 A Resolution


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Endopolygalacturonase I From Stereum Purpureum at 0.96 A Resolution within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl2003 b:13.7 occ:1.00 OG1 A:THR55 3.1 14.3 1.0 N A:THR55 3.3 11.7 1.0 N A:GLY30 3.4 13.9 1.0 CA A:GLY30 3.7 15.9 1.0 OG1 A:THR54 3.7 11.9 1.0 CB A:THR55 3.8 13.2 1.0 CG2 A:THR55 3.9 14.0 1.0 CB A:ALA29 4.0 13.8 1.0 C A:ALA29 4.0 12.9 1.0 CA A:THR55 4.1 12.3 1.0 CA A:THR54 4.1 11.1 1.0 C A:THR54 4.2 11.3 1.0 O A:HOH2486 4.4 37.6 0.5 CA A:ALA29 4.4 12.8 1.0 CB A:THR54 4.5 11.1 1.0 N A:LEU56 4.6 12.7 1.0 C A:THR55 4.7 12.3 1.0 O A:ALA29 4.7 15.0 1.0 O A:HOH2180 4.9 24.0 1.0

T.Shimizu, T.Nakatsu, K.Miyairi, T.Okuno, H.Kato. Active-Site Architecture of Endopolygalacturonase I From Stereum Purpureum Revealed By Crystal Structures in Native and Ligand-Bound Forms at Atomic Resolution. Biochemistry V. 41 6651 2002.
ISSN: ISSN 0006-2960
PubMed: 12022868
DOI: 10.1021/BI025541A