Chlorine in PDB 1kay: 70KD Heat Shock Cognate Protein Atpase Domain, K71A Mutant

All present enzymatic activity of 70KD Heat Shock Cognate Protein Atpase Domain, K71A Mutant:
3.6.1.3;

The structure of 70KD Heat Shock Cognate Protein Atpase Domain, K71A Mutant, PDB code: 1kay was solved by M.C.O'brien, K.M.Flaherty, D.B.Mckay, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	6.00 / 1.70
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	143.900, 64.600, 46.200, 90.00, 90.00, 90.00
R / Rfree (%)	20.4 / 24.3

The structure of 70KD Heat Shock Cognate Protein Atpase Domain, K71A Mutant also contains other interesting chemical elements:

Magnesium	(Mg)	1 atom
Potassium	(K)	3 atoms

The binding sites of Chlorine atom in the 70KD Heat Shock Cognate Protein Atpase Domain, K71A Mutant (pdb code 1kay). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 2 binding sites of Chlorine where determined in the 70KD Heat Shock Cognate Protein Atpase Domain, K71A Mutant, PDB code: 1kay:
Jump to Chlorine binding site number: 1; 2;

Chlorine binding site 1 out of 2 in the 70KD Heat Shock Cognate Protein Atpase Domain, K71A Mutant


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of 70KD Heat Shock Cognate Protein Atpase Domain, K71A Mutant within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl451 b:18.6 occ:1.00 NZ A:LYS345 3.0 21.9 1.0 O A:HOH521 3.1 7.6 1.0 NZ A:LYS348 3.5 11.3 1.0 CE A:LYS345 3.5 17.9 1.0 NH2 A:ARG311 3.8 21.1 1.0 CD A:LYS348 3.8 12.9 1.0 CE A:LYS348 4.0 13.3 1.0 CD2 A:LEU349 4.2 8.4 1.0 CD A:LYS345 4.3 14.4 1.0 CG A:LYS348 4.3 10.8 1.0 OD2 A:ASP352 4.4 5.5 1.0 CG A:LEU349 4.5 8.5 1.0

Chlorine binding site 2 out of 2 in the 70KD Heat Shock Cognate Protein Atpase Domain, K71A Mutant


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of 70KD Heat Shock Cognate Protein Atpase Domain, K71A Mutant within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl455 b:15.2 occ:1.00 N A:ASP32 3.3 14.5 1.0 O A:HOH556 3.3 10.1 1.0 NE2 A:GLN33 3.3 11.9 1.0 NZ A:LYS126 3.4 13.8 1.0 CB A:ASN31 3.4 8.2 1.0 CG A:ASP32 3.5 25.5 1.0 CB A:ASP32 3.5 19.5 1.0 OD1 A:ASP32 3.7 28.8 1.0 CD A:LYS126 3.8 12.4 1.0 CG A:ASN31 3.8 6.1 1.0 OD2 A:ASP32 3.8 27.7 1.0 O A:HOH660 3.9 21.2 1.0 CA A:ASP32 4.0 15.8 1.0 O A:HOH661 4.0 23.2 1.0 CE A:LYS126 4.0 13.4 1.0 OE1 A:GLN33 4.1 17.2 1.0 CD A:GLN33 4.1 15.4 1.0 ND2 A:ASN31 4.1 5.1 1.0 C A:ASN31 4.2 12.1 1.0 O A:HOH635 4.2 11.0 1.0 CG1 A:ILE130 4.3 12.9 1.0 CA A:ASN31 4.3 11.4 1.0 CD1 A:ILE130 4.4 12.2 1.0 OD1 A:ASN31 4.4 10.8 1.0 O A:HOH751 4.5 33.1 1.0 N A:GLN33 4.7 14.4 1.0 O A:HOH633 4.7 16.6 1.0 C A:ASP32 4.8 16.5 1.0 CG A:LYS126 5.0 12.6 1.0

M.C.O'brien, K.M.Flaherty, D.B.Mckay. Lysine 71 of the Chaperone Protein HSC70 Is Essential For Atp Hydrolysis. J.Biol.Chem. V. 271 15874 1996.
ISSN: ISSN 0021-9258
PubMed: 8663302
DOI: 10.1074/JBC.271.27.15874