Chlorine in PDB 1nw8: Structure of L72P Mutant Beta Class N6-Adenine Dna Methyltransferase Rsri

All present enzymatic activity of Structure of L72P Mutant Beta Class N6-Adenine Dna Methyltransferase Rsri:
2.1.1.72;

The structure of Structure of L72P Mutant Beta Class N6-Adenine Dna Methyltransferase Rsri, PDB code: 1nw8 was solved by C.B.Thomas, R.D.Scavetta, R.I.Gumport, M.E.A.Churchill, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	35.80 / 2.25
Space group	C 2 2 21
Cell size a, b, c (Å), α, β, γ (°)	71.600, 131.380, 67.540, 90.00, 90.00, 90.00
R / Rfree (%)	19.5 / 23.9

The binding sites of Chlorine atom in the Structure of L72P Mutant Beta Class N6-Adenine Dna Methyltransferase Rsri (pdb code 1nw8). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 2 binding sites of Chlorine where determined in the Structure of L72P Mutant Beta Class N6-Adenine Dna Methyltransferase Rsri, PDB code: 1nw8:
Jump to Chlorine binding site number: 1; 2;

Chlorine binding site 1 out of 2 in the Structure of L72P Mutant Beta Class N6-Adenine Dna Methyltransferase Rsri


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Structure of L72P Mutant Beta Class N6-Adenine Dna Methyltransferase Rsri within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl501 b:33.9 occ:1.00 OG A:SER253 3.0 37.6 1.0 N A:SER253 3.5 35.5 1.0 CE A:LYS227 3.5 49.7 1.0 CB A:SER253 3.7 34.6 1.0 N A:LYS227 3.9 43.4 1.0 CA A:GLN226 3.9 45.4 1.0 CG1 A:VAL255 3.9 20.0 1.0 O A:ALA251 4.0 22.2 1.0 O A:HOH560 4.1 26.6 1.0 CA A:SER253 4.2 36.1 1.0 NZ A:LYS227 4.2 50.3 1.0 C A:GLN226 4.4 44.7 1.0 C A:GLY252 4.5 34.3 1.0 ND1 A:HIS223 4.5 68.0 1.0 CA A:GLY252 4.5 28.7 1.0 CG A:GLN226 4.5 44.4 1.0 O A:THR225 4.5 46.2 1.0 CE1 A:HIS223 4.6 68.7 1.0 CB A:GLN226 4.7 46.1 1.0 N A:GLN226 4.7 46.6 1.0 CB A:VAL255 4.7 20.5 1.0 CG A:HIS223 4.7 67.2 1.0 CD A:LYS227 4.8 48.7 1.0 C A:ALA251 4.8 21.4 1.0 N A:GLY254 4.8 34.7 1.0 CG A:LYS227 4.8 46.6 1.0 NE2 A:HIS223 4.9 68.8 1.0 CB A:LYS227 4.9 44.6 1.0 C A:SER253 4.9 36.0 1.0 C A:THR225 4.9 47.8 1.0 O A:HOH508 5.0 16.4 1.0 OD2 A:ASP65 5.0 19.4 1.0 CD2 A:HIS223 5.0 67.7 1.0 CA A:LYS227 5.0 43.8 1.0

Chlorine binding site 2 out of 2 in the Structure of L72P Mutant Beta Class N6-Adenine Dna Methyltransferase Rsri


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Structure of L72P Mutant Beta Class N6-Adenine Dna Methyltransferase Rsri within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl502 b:31.0 occ:1.00 CB A:ARG212 3.1 49.9 1.0 N A:ARG212 3.2 43.2 1.0 N A:ASN144 3.3 32.2 1.0 O A:ASN144 3.5 35.8 1.0 CB A:TYR142 3.5 20.9 1.0 CD A:PRO143 3.7 32.7 1.0 CA A:ARG212 3.7 46.8 1.0 N A:PRO143 3.9 29.7 1.0 CA A:ASN144 4.0 33.9 1.0 CB A:ASN144 4.2 38.9 1.0 C A:ASN144 4.2 35.1 1.0 CD1 A:TYR142 4.2 18.9 1.0 CG A:TYR142 4.2 20.9 1.0 C A:TYR142 4.2 26.9 1.0 C A:SER211 4.2 39.4 1.0 CG A:ARG212 4.3 56.8 1.0 CD A:ARG212 4.3 63.0 1.0 CA A:SER211 4.3 37.2 1.0 C A:PRO143 4.3 32.2 1.0 CA A:TYR142 4.4 23.8 1.0 O A:ARG212 4.4 46.1 1.0 CA A:PRO143 4.5 31.5 1.0 ND2 A:ASN144 4.5 42.8 1.0 C A:ARG212 4.5 47.8 1.0 CB A:PRO143 4.5 32.6 1.0 CG A:PRO143 4.7 34.8 1.0 CG A:ASN144 4.7 42.6 1.0 CB A:SER211 4.9 35.7 1.0 O A:TYR142 4.9 26.9 1.0

C.B.Thomas, R.D.Scavetta, R.I.Gumport, M.E.A.Churchill. Structures of Liganded and Unliganded Rsri N6-Adenine Dna Methyltransferase: A Distinct Orientation For Active Cofactor Binding J.Biol.Chem. V. 278 26094 2003.
ISSN: ISSN 0021-9258
PubMed: 12732637
DOI: 10.1074/JBC.M303751200