Chlorine in PDB 1rnx: Ribonuclease A Crystallized From 3M Sodium Chloride, 30% Ammonium Sulfate

All present enzymatic activity of Ribonuclease A Crystallized From 3M Sodium Chloride, 30% Ammonium Sulfate:
3.1.27.5;

The structure of Ribonuclease A Crystallized From 3M Sodium Chloride, 30% Ammonium Sulfate, PDB code: 1rnx was solved by A.A.Fedorov, D.Joseph-Mccarthy, L.Fedorov, D.Sirakova, I.Graf, S.C.Almo, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	8.00 / 1.90
Space group	P 32 2 1
Cell size a, b, c (Å), α, β, γ (°)	64.659, 64.659, 65.254, 90.00, 90.00, 120.00
R / Rfree (%)	n/a / n/a

The binding sites of Chlorine atom in the Ribonuclease A Crystallized From 3M Sodium Chloride, 30% Ammonium Sulfate (pdb code 1rnx). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 4 binding sites of Chlorine where determined in the Ribonuclease A Crystallized From 3M Sodium Chloride, 30% Ammonium Sulfate, PDB code: 1rnx:
Jump to Chlorine binding site number: 1; 2; 3; 4;

Chlorine binding site 1 out of 4 in the Ribonuclease A Crystallized From 3M Sodium Chloride, 30% Ammonium Sulfate


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Ribonuclease A Crystallized From 3M Sodium Chloride, 30% Ammonium Sulfate within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl125 b:29.9 occ:1.00 O A:HOH232 2.9 29.9 1.0 NE2 A:HIS12 3.1 7.5 1.0 N A:PHE120 3.3 11.5 1.0 O A:HOH279 3.5 47.1 1.0 CA A:HIS119 3.7 13.9 1.0 ND1 A:HIS119 3.9 32.7 1.0 NE2 A:GLN11 3.9 20.0 1.0 CE1 A:HIS12 4.0 4.3 1.0 C A:HIS119 4.0 13.9 1.0 CD2 A:HIS12 4.1 7.7 1.0 CB A:PHE120 4.2 11.5 1.0 CA A:PHE120 4.2 12.1 1.0 O A:PHE120 4.3 16.3 1.0 CB A:HIS119 4.3 20.2 1.0 CG A:HIS119 4.5 29.1 1.0 O A:VAL118 4.6 15.2 1.0 C A:PHE120 4.8 14.2 1.0 CE1 A:HIS119 4.8 33.8 1.0 N A:HIS119 4.9 14.0 1.0

Chlorine binding site 2 out of 4 in the Ribonuclease A Crystallized From 3M Sodium Chloride, 30% Ammonium Sulfate


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Ribonuclease A Crystallized From 3M Sodium Chloride, 30% Ammonium Sulfate within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl126 b:27.7 occ:1.00 ND2 A:ASN34 3.0 25.9 1.0 O A:HOH291 3.4 54.4 1.0 CD A:ARG10 3.4 10.5 1.0 NE A:ARG10 3.8 11.7 1.0 CG A:ASN34 3.9 22.9 1.0 CB A:ASN34 3.9 21.1 1.0 CZ A:ARG10 4.2 10.8 1.0 NH1 A:ARG10 4.3 13.7 1.0 O A:ARG33 4.6 15.5 1.0 CG A:ARG10 4.8 9.0 1.0

Chlorine binding site 3 out of 4 in the Ribonuclease A Crystallized From 3M Sodium Chloride, 30% Ammonium Sulfate


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 3 of Ribonuclease A Crystallized From 3M Sodium Chloride, 30% Ammonium Sulfate within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl127 b:23.1 occ:1.00 O A:HOH279 3.1 47.1 1.0 N A:THR45 3.3 9.6 1.0 CE1 A:HIS12 3.6 4.3 1.0 OG1 A:THR45 3.7 12.5 1.0 CA A:ASN44 3.8 11.4 1.0 C A:ASN44 3.9 11.4 1.0 CD1 A:PHE120 4.0 11.7 1.0 CB A:THR45 4.0 10.8 1.0 CB A:PHE120 4.2 11.5 1.0 ND1 A:HIS12 4.2 6.0 1.0 CA A:THR45 4.3 8.5 1.0 OD1 A:ASN44 4.4 12.3 1.0 O A:VAL43 4.4 13.9 1.0 CG A:PHE120 4.4 11.7 1.0 N A:ASN44 4.4 10.9 1.0 O A:PHE120 4.6 16.3 1.0 NE2 A:HIS12 4.6 7.5 1.0 C A:VAL43 4.6 12.6 1.0 NZ A:LYS41 4.7 26.1 1.0 CE1 A:PHE120 4.8 12.7 1.0 CG A:ASN44 4.8 11.1 1.0 CB A:ASN44 4.9 11.1 1.0 CG1 A:VAL43 4.9 14.9 1.0 O A:THR45 5.0 8.6 1.0

Chlorine binding site 4 out of 4 in the Ribonuclease A Crystallized From 3M Sodium Chloride, 30% Ammonium Sulfate


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 4 of Ribonuclease A Crystallized From 3M Sodium Chloride, 30% Ammonium Sulfate within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl128 b:28.8 occ:1.00 OG1 A:THR3 3.0 11.5 1.0 O A:HOH237 3.3 29.4 1.0 O A:HOH241 3.7 32.3 1.0 CB A:ALA5 3.9 11.4 1.0 CB A:THR3 4.0 13.0 1.0 CG2 A:THR3 4.1 12.5 1.0 N A:ALA6 4.2 10.8 1.0 CB A:ALA6 4.5 9.6 1.0 O A:HOH216 4.7 22.6 1.0 CA A:ALA6 4.8 9.2 1.0 C A:ALA5 4.8 11.0 1.0 CA A:ALA5 4.9 11.0 1.0

A.A.Fedorov, D.Joseph-Mccarthy, E.Fedorov, D.Sirakova, I.Graf, S.C.Almo. Ionic Interactions in Crystalline Bovine Pancreatic Ribonuclease A. Biochemistry V. 35 15962 1996.
ISSN: ISSN 0006-2960
PubMed: 8973167
DOI: 10.1021/BI961533G